Imperial College London
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DrSarahJoseph

Faculty of Medicine, Department of Infectious Disease

Clinical Immunologist
 
 
 
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s.joseph

 
 
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Location

 

457Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kyongo:2015:10.1128/cvi.00762-14,
author = {Kyongo, JK and Crucitti, T and Menten, J and Hardy, L and Cools, P and Michiels, J and Delany-Moretlwe, S and Mwaura, M and Ndayisaba, G and Joseph, S and Fichorova, R and van, de Wijgert J and Vanham, G and Ariën, KK and Jespers, V},
doi = {10.1128/cvi.00762-14},
journal = {Clinical and Vaccine Immunology},
pages = {526--538},
title = {Cross-Sectional Analysis of Selected Genital Tract Immunological Markers and Molecular Vaginal Microbiota in Sub-Saharan African Women, with Relevance to HIV Risk and Prevention},
url = {http://dx.doi.org/10.1128/cvi.00762-14},
volume = {22},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>ABSTRACT</jats:title><jats:p>Data on immune mediators in the genital tract and the factors that modulate them in sub-Saharan women are limited. Cervicovaginal lavage (CVL) samples from 430 sexually active women from Kenya, South Africa, and Rwanda were analyzed for 12 soluble immune mediators using Bio-Plex and Meso Scale Discovery multiplex platforms, as well as single enzyme-linked immunosorbent assays. Ten bacterial species were quantified in vaginal swab samples. Bacterial vaginosis (BV) was defined by Nugent scoring. CVL samples from HIV-infected women showed a clear-cut proinflammatory profile. Pregnant women, adolescents, and women engaging in traditional vaginal practices differed in specific soluble markers compared to reference groups of adult HIV-negative women. Cervical mucus, cervical ectopy, abnormal vaginal discharge, and having multiple sex partners were each associated with an increase in inflammatory mediators. The levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-12(p70), and IL-8 were elevated, whereas the IL-1RA/IL-1(α+β) ratio decreased in women with BV. The level of gamma interferon-induced protein 10 was lower in BV-positive than in BV-negative women, suggesting its suppression as a potential immune evasion mechanism by BV-associated bacteria.<jats:named-content content-type="genus-species">Lactobacillus crispatus</jats:named-content>and<jats:named-content content-type="genus-species">Lactobacillus vaginalis</jats:named-content>were associated with decreased proinflammatory cytokines and each BV-associated species with increased proinflammatory cytokines. Remarkably, the<jats:italic>in vitro</jats:italic>anti-HIV activity of CVL samples from BV-positive women was stronger than that of BV-negative women. In conclusion, we found significant associations of factors, including vaginal microbiota, which can influence immune mediators i
AU  - Kyongo,JK
AU  - Crucitti,T
AU  - Menten,J
AU  - Hardy,L
AU  - Cools,P
AU  - Michiels,J
AU  - Delany-Moretlwe,S
AU  - Mwaura,M
AU  - Ndayisaba,G
AU  - Joseph,S
AU  - Fichorova,R
AU  - van,de Wijgert J
AU  - Vanham,G
AU  - Ariën,KK
AU  - Jespers,V
DO  - 10.1128/cvi.00762-14
EP  - 538
PY  - 2015///
SN  - 1556-6811
SP  - 526
TI  - Cross-Sectional Analysis of Selected Genital Tract Immunological Markers and Molecular Vaginal Microbiota in Sub-Saharan African Women, with Relevance to HIV Risk and Prevention
T2  - Clinical and Vaccine Immunology
UR  - http://dx.doi.org/10.1128/cvi.00762-14
VL  - 22
ER  -
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