Imperial College London
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DrSarahJoseph

Faculty of Medicine, Department of Infectious Disease

Clinical Immunologist
 
 
 
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s.joseph

 
 
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Location

 

457Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cosgrove:2016:10.1371/journal.pone.0152038,
author = {Cosgrove, CA and Lacey, CJ and Cope, AV and Bartolf, A and Morris, G and Yan, C and Baden, S and Cole, T and Carter, D and Brodnicki, E and Shen, X and Joseph, S and DeRosa, SC and Peng, L and Yu, X and Ferrari, G and Seaman, M and Montefiori, DC and Frahm, N and Tomaras, GD and Stöhr, W and McCormack, S and Shattock, RJ},
doi = {10.1371/journal.pone.0152038},
journal = {PLOS One},
title = {Comparative immunogenicity of HIV-1 gp140 vaccine delivered by parenteral, and mucosal routes in female volunteers; MUCOVAC2, a randomized two centre study},
url = {http://dx.doi.org/10.1371/journal.pone.0152038},
volume = {11},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundDefining optimal routes for induction of mucosal immunity represents an important research priority for the HIV-1 vaccine field. In particular, it remains unclear whether mucosal routes of immunization can improve mucosal immune responses.MethodsIn this randomized two center phase I clinical trial we evaluated the systemic and mucosal immune response to a candidate HIV-1 Clade C CN54gp140 envelope glycoprotein vaccine administered by intramuscular (IM), intranasal (IN) and intravaginal (IVAG) routes of administration in HIV negative female volunteers. IM immunizations were co-administered with Glucopyranosyl Lipid Adjuvant (GLA), IN immunizations with 0.5% chitosan and IVAG immunizations were administered in an aqueous gel.ResultsThree IM immunizations of CN54 gp140 at either 20 or 100 μg elicited significantly greater systemic and mucosal antibodies than either IN or IVAG immunizations. Following additional intramuscular boosting we observed an anamnestic antibody response in nasally primed subjects. Modest neutralizing responses were detected against closely matched tier 1 clade C virus in the IM groups. Interestingly, the strongest CD4 T-cell responses were detected after IN and not IM immunization.ConclusionsThese data show that parenteral immunization elicits systemic and mucosal antibodies in women. Interestingly IN immunization was an effective prime for IM boost, while IVAG administration had no detectable impact on systemic or mucosal responses despite IM priming.
AU  - Cosgrove,CA
AU  - Lacey,CJ
AU  - Cope,AV
AU  - Bartolf,A
AU  - Morris,G
AU  - Yan,C
AU  - Baden,S
AU  - Cole,T
AU  - Carter,D
AU  - Brodnicki,E
AU  - Shen,X
AU  - Joseph,S
AU  - DeRosa,SC
AU  - Peng,L
AU  - Yu,X
AU  - Ferrari,G
AU  - Seaman,M
AU  - Montefiori,DC
AU  - Frahm,N
AU  - Tomaras,GD
AU  - Stöhr,W
AU  - McCormack,S
AU  - Shattock,RJ
DO  - 10.1371/journal.pone.0152038
PY  - 2016///
SN  - 1932-6203
TI  - Comparative immunogenicity of HIV-1 gp140 vaccine delivered by parenteral, and mucosal routes in female volunteers; MUCOVAC2, a randomized two centre study
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0152038
UR  - http://hdl.handle.net/10044/1/32512
VL  - 11
ER  -
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