Imperial College London
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DrSarahJoseph

Faculty of Medicine, Department of Infectious Disease

Clinical Immunologist
 
 
 
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s.joseph

 
 
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Location

 

457Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haidari:2017:10.1038/s41598-017-13331-1,
author = {Haidari, G and Cope, A and Miller, A and Venables, S and Yan, C and Ridgers, H and Reijonen, K and Hannaman, D and Spentzou, A and Hayes, P and Bouliotis, G and Vogt, A and Joseph, S and Combadiere, B and McCormack, S and Shattock, RJ},
doi = {10.1038/s41598-017-13331-1},
journal = {SCIENTIFIC REPORTS},
title = {Combined skin and muscle vaccination differentially impact the quality of effector T cell functions: the CUTHIVAC-001 randomized trial},
url = {http://dx.doi.org/10.1038/s41598-017-13331-1},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Targeting of different tissues via transcutaneous (TC), intradermal (ID) and intramuscular (IM) injection has the potential to tailor the immune response to DNA vaccination. In this Phase I randomised controlled clinical trial in HIV-1 negative volunteers we investigate whether the site and mode of DNA vaccination influences the quality of the cellular immune responses. We adopted a strategy of concurrent immunization combining IM injection with either ID or TC administration. As a third arm we assessed the response to IM injection administered with electroporation (EP). The DNA plasmid encoded a MultiHIV B clade fusion protein designed to induce cellular immunity. The vaccine and regimens were well tolerated. We observed differential shaping of vaccine induced virus-specific CD4 + and CD8 + cell-mediated immune responses. DNA given by IM + EP promoted strong IFN-γ responses and potent viral inhibition. ID + IM without EP resulted in a similar pattern of response but of lower magnitude. By contrast TC + IM (without EP) shifted responses towards a more Th-17 dominated phenotype, associated with mucosal and epidermal protection. Whilst preliminary, these results offer new perspectives for differential shaping of desired cellular immunity required to fight the wide range of complex and diverse infectious diseases and cancers.
AU  - Haidari,G
AU  - Cope,A
AU  - Miller,A
AU  - Venables,S
AU  - Yan,C
AU  - Ridgers,H
AU  - Reijonen,K
AU  - Hannaman,D
AU  - Spentzou,A
AU  - Hayes,P
AU  - Bouliotis,G
AU  - Vogt,A
AU  - Joseph,S
AU  - Combadiere,B
AU  - McCormack,S
AU  - Shattock,RJ
DO  - 10.1038/s41598-017-13331-1
PY  - 2017///
SN  - 2045-2322
TI  - Combined skin and muscle vaccination differentially impact the quality of effector T cell functions: the CUTHIVAC-001 randomized trial
T2  - SCIENTIFIC REPORTS
UR  - http://dx.doi.org/10.1038/s41598-017-13331-1
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000412950600002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/55274
VL  - 7
ER  -
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