Imperial College London

Professor Sergei Kazarian

Faculty of EngineeringDepartment of Chemical Engineering

Professor of Physical Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5574s.kazarian Website

 
 
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Assistant

 

Mrs Sarah Payne +44 (0)20 7594 5567

 
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Location

 

440Bone BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

374 results found

Beattie JW, Istrate A, Lu A, Marshall C, Rowland-Jones RC, Farys M, Kazarian SG, Byrne Bet al., 2022, Causes of industrial protein a column degradation, explored using Raman spectroscopy., Analytical Chemistry, Vol: 94, Pages: 15703-15710, ISSN: 0003-2700

Monoclonal antibodies (mAbs) are used extensively as biotherapeutics for chronic and acute conditions. Production of mAbs is lengthy and expensive, with protein A affinity capture the most costly step, due both to the nature of the resin and its marked reduction in binding capacity with repeated use. Our previous studies using in situ ATR-FTIR spectroscopy indicated that loss in protein A binding capacity is not the result of leaching or degradation of protein A ligand, suggesting fouling is the principal cause. Here we explore binding behavior and resin capacity loss using Raman spectroscopy. Our data reveal a distinct Raman spectral fingerprint for mAb bound to the protein A ligand of MabSelect SuRe. The results show that the drop in static binding capacity (SBC) previously observed for used protein A resin is discernible by Raman spectroscopy in combination with partial least-squares regression. The SBC is lowest (35.76 mg mL-1) for used inlet resin compared to used outlet (40.17 mg mL-1) and unused resin samples (70.35 mg mL-1). Depth profiling by Raman spectroscopy indicates that at below saturating concentrations (∼18 mg mL-1), binding of mAb is not homogeneous through used resin beads with protein binding preferentially to the outer regions of the bead, in contrast to fully homogeneous distribution through unused control MabSelect SuRe resin beads. Analysis of the Raman spectra indicates that one foulant is irreversibly bound mAb. The presence of irreversibly bound mAb and host cell proteins was confirmed by mass spectrometric analysis of used resin beads.

Journal article

Tiernan H, Friedman S, Clube RKM, Burgman MA, Castillo AC, Stettler MEJ, Kazarian SG, Wright S, De Nazelle Aet al., 2022, Implementation of a structured decision-making framework to evaluate and advance understanding of airborne microplastics, Environmental Science and Policy, Vol: 135, Pages: 169-181, ISSN: 1462-9011

Microplastic pollution is increasingly recognised as a global environmental challenge which stems from the rapid growth of the use of petrochemical-derived plastic. As researchers and practitioners face a myriad of environmental challenges, oceanic microplastic pollution has so far dominated interest. However, airborne microplastics present an increasing environmental and public health concern. There is currently a need for research addressing this emerging challenge, and at the same time, the lack of knowledge and consensus regarding airborne microplastics presents an obstacle to action. The purpose of this study is to utilise a participatory Structured Decision-Making (SDM) approach to understand the perspectives of a range of stakeholders involved in the microplastics landscape, and subsequently refine common research priorities and knowledge gaps to advance the field. Through two participatory workshops, we first defined shared objectives of stakeholders and then negotiated best courses of action to achieve these objectives based on discussion between stakeholders and facilitators. The qualitative approach taken has enabled the full, complex and multidisciplinary aspects of the research into airborne microplastic pollution to be considered. Our findings highlight some important potential consequences of airborne microplastic pollution, including impacts on human health, and the need for more interdisciplinary research, and collaborative, integrated approaches in this area. As a result of the first workshop, five fundamental objectives on the theme of airborne microplastics were identified. As a direct consequence of this, participants identified 84 actions split across eight themes, which are outlined later in this paper.

Journal article

Shalygin AS, Milovanov ES, Kovalev EP, Yakushkin SS, Kazarian SG, Martyanov ONet al., 2022, In Situ FTIR Spectroscopic Imaging of Asphaltene Deposition from Crude Oil under n-Heptane and Acetone Flows, PETROLEUM CHEMISTRY, Vol: 62, Pages: 1087-1095, ISSN: 0965-5441

Journal article

Kharintsev SS, Kharitonov A, Chernykh EA, Alekseev AM, Filippov NA, Kazarian SGet al., 2022, Designing two-dimensional temperature profiles using tunable thermoplasmonics, NANOSCALE, Vol: 14, Pages: 12117-12128, ISSN: 2040-3364

Journal article

V Solovyeva E, Smirnov AN, Svinko VO, Strelnikov AS, Shevchuk AI, Kazarian SGet al., 2022, Unraveling a role of molecular linker in nanoparticles self-organization by SERS spectroscopy: Comparative study of three aromatic diamines, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, Vol: 645, ISSN: 0927-7757

Journal article

Kharintsev SS, Kazarian SG, 2022, Nanoscale Melting of 3D Confined Azopolymers through Tunable Thermoplasmonics, JOURNAL OF PHYSICAL CHEMISTRY LETTERS, Vol: 13, Pages: 5351-5357, ISSN: 1948-7185

Journal article

Liu G-L, Kazarian SG, 2022, Recent advances and applications to cultural heritage using ATR-FTIR spectroscopy and ATR-FTIR spectroscopic imaging, ANALYST, Vol: 147, Pages: 1777-1797, ISSN: 0003-2654

Journal article

Kovalev EP, Shalygin AS, Shubin AA, Kozhevnikov I, Prikhod'ko SA, Adonin NY, Kazarian SG, Martyanov ONet al., 2022, Interactions of C2H6 and C2H4 with the homologous series [CnMIM][BF4] ionic liquids at high pressure studied by in situ ATR-FTIR spectroscopy, JOURNAL OF MOLECULAR LIQUIDS, Vol: 348, ISSN: 0167-7322

Journal article

Possenti E, Colombo C, Realini M, Song CL, Kazarian SGet al., 2021, Time-Resolved ATR-FTIR Spectroscopy and Macro ATR-FTIR Spectroscopic Imaging of Inorganic Treatments for Stone Conservation, ANALYTICAL CHEMISTRY, Vol: 93, Pages: 14635-14642, ISSN: 0003-2700

Journal article

Paraskevaidi M, Matthew BJ, Holly BJ, Hugh BJ, Thulya CPV, Loren C, StJohn C, Peter G, Callum G, Sergei KG, Kamila K, Maria K, Kassio LMG, Pierre M-HL, Evangelos P, Savithri P, John AA, Alexandra S, Marfran S, Josep S-S, Gunjan T, Michael W, Bayden Wet al., 2021, Clinical applications of infrared and Raman spectroscopy in the fields of cancer and infectious diseases, Applied Spectroscopy Reviews, Vol: 56, Pages: 804-868, ISSN: 0570-4928

Analytical technologies that can improve disease diagnosis are highly sought after. Current screening/diagnostic tests for several diseases are limited by their moderate diagnostic performance, invasiveness, costly and laborious methodologies or the need for multiple tests before a definitive diagnosis. Spectroscopic techniques, including infrared (IR) and Raman, have attracted great interest in the medical field, with applications expanding from early disease detection to monitoring and real-time diagnosis. This review highlights applications of IR and Raman spectroscopy, with a focus on cancer and infectious diseases since 2015, and underscores the diverse sample types that can be analyzed, such as biofluids, cells and tissues. Studies involving more than 25 participants per group (disease and control group; if no control group >25 in disease group) were considered eligible, to retain the clinical focus of the paper. Following literature searches, we identified 94 spectroscopic studies on different cancers and 30 studies on infectious diseases. The review suggests that such technologies have the potential to develop into an objective, inexpensive, point-of-care test or facilitate disease diagnosis and monitoring. Up-to-date considerations for the implementation of spectroscopic techniques into a clinical setting, health economics and successful applications of vibrational spectroscopic tests in the clinical arena are also discussed.

Journal article

Beattie J, Rowland-Jones R, Farys M, Kucia-Tran R, Kazarian S, Byrne Bet al., 2021, Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy, The Analyst, Vol: 146, Pages: 5177-5185, ISSN: 0003-2654

Therapeutic monoclonal antibodies (mAbs) are effective treatments for a range of cancers and other serious diseases, however mAb treatments cost on average ∼$100 000 per year per patient, limiting their use. Currently, industry favours Protein A affinity chromatography (PrAc) as the key step in downstream processing of mAbs. This step, although highly efficient, represents a significant mAb production cost. Fouling of the Protein A column and Protein A ligand leaching contribute to the cost of mAb production by shortening the life span of the resin. In this study, we assessed the performance of used PrAc resin recovered from the middle inlet, center and outlet as well as the side inlet of a pilot-scale industrial column. We used a combination of static binding capacity (SBC) analysis and Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopy to explore the used resin samples. SBC analysis demonstrated that resin from the inlet of the column had lower binding capacity than resin from the column outlet. ATR-FTIR spectroscopy with PLS (partial least square) analysis confirmed the results obtained from SBC analysis. Importantly, in situ ATR-FTIR spectroscopy also allowed both measurement of the concentration and assessment of the conformational state of the bound Protein A. Our results reveal that PrAc resin degradation after use is dependent on column location and that neither Protein A ligand leaching nor denaturation are responsible for binding capacity loss.

Journal article

Paukshtis EA, Kovalyov EV, Glazneva TS, Kazarian SGet al., 2021, New DRIFT spectroscopic methodology for acquiring infrared spectra of fiberglass materials, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Vol: 260, ISSN: 1386-1425

Journal article

Chan KLA, Shalygin AS, Martyanov ON, Welton T, Kazarian SGet al., 2021, High throughput study of ionic liquids in controlled environments with FTIR spectroscopic imaging, JOURNAL OF MOLECULAR LIQUIDS, Vol: 337, ISSN: 0167-7322

Journal article

Tiernan H, Byrne B, Kazarian SG, 2021, ATR-FTIR spectroscopy and spectroscopic imaging to investigate the behaviour of proteins subjected to freeze-thaw cycles in droplets, wells, and under flow., The Analyst, Vol: 146, Pages: 2902-2909, ISSN: 0003-2654

Biopharmaceuticals are used to treat a range of diseases from arthritis to cancer, however, since the advent of these highly specific, effective drugs, there have been challenges involved in their production. The most common biopharmaceuticals, monoclonal antibodies (mAbs), are vulnerable to aggregation and precipitation during processing. Freeze thaw cycles (FTCs), which can be required for storage and transportation, can lead to a substantial loss of product, and contributes to the high cost of antibody production. It is therefore necessary to monitor aggregation levels at susceptible points in the production pathway, such as during purification and transportation, thus contributing to a fuller understanding of mAb aggregation and providing a basis for rational optimisation of the production process. This paper uses attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and spectroscopic imaging to investigate the effect of these potentially detrimental FTCs on protein secondary structure in both static wells and under flowing conditions, using lysozyme as a model protein. The results revealed that the amount of protein close to the surface of the ATR crystal, and hence level of aggregates, increased with increasing FTCs. This was observed both within wells and under flow conditions, using conventional ATR-FTIR spectroscopy and ATR-FTIR spectroscopic imaging. Interestingly, we also observed changes in the Amide I band shape indicating an increase in β-sheet contribution, and therefore an increase in aggregates, with increasing number of FTCs. These results show for the first time how ATR-FTIR spectroscopy can be successfully applied to study the effect of FTC cycles on protein samples. This could have numerous broader applications, such as in biopharmaceutical production and rapid diagnostic testing.

Journal article

Kharintsev SS, Chernykh EA, Shelaev A, Kazarian SGet al., 2021, Nanoscale Sensing Vitrification of 3D Confined Glassy Polymers Through Refractory Thermoplasmonics, ACS PHOTONICS, Vol: 8, Pages: 1477-1488, ISSN: 2330-4022

Journal article

Yu Z, Jiang N, Kazarian SG, Tasoglu S, Yetisen AKet al., 2021, Optical sensors for continuous glucose monitoring, Progress in Biomedical Engineering, Vol: 3, Pages: 022004-022004

For decades, diabetes mellitus has been of wide concern with its high global prevalence, resulting in increasing social and financial burdens for individuals, clinical systems and governments. Continuous glucose monitoring (CGM) has become a popular alternative to the portable finger-prick glucometers available in the market for the convenience of diabetic patients. Hence, it has attracted much interest in various glucose sensing technologies to develop novel glucose sensors with better performance and longer lifetime, especially non-invasive or minimally invasive glucose sensing. Effort has also been put into finding biocompatible materials for implantable applications to achieve effective in vivo CGM. Here, we review the state-of-the-art researches in the field of CGM. The currently commercially available CGM technologies have been analyzed and a summary is provided of the potential types of recently researched non-invasive glucose monitors. Furthermore, the challenges and advances towards implantable applications have also been introduced and discussed, especially the novel biocompatible hydrogel aimed at minimizing the adverse impact from foreign-body response. In addition, a large variety of promising glucose-sensing technologies under research have been reviewed, from traditional electrochemical-based glucose sensors to novel optical and other electrical glucose sensors. The recent development and achievement of the reviewed glucose sensing technologies are discussed, together with the market analysis in terms of the statistical data for the newly published patents in the related field. Thus, the promising direction for future work in this field could be concluded.

Journal article

Ishige R, Song CL, Hara S, Ando S, Kazarian SGet al., 2021, Analysis of spatial orientation distribution of highly oriented polyimide film using micro ATR-FTIR spectroscopic imaging method, POLYMER, Vol: 221, ISSN: 0032-3861

Journal article

Lu H, Shinzawa H, Kazarian SG, 2021, Intermolecular Interactions in the Polymer Blends Under High-Pressure CO2 Studied Using Two-Dimensional Correlation Analysis and Two-Dimensional Disrelation Mapping, APPLIED SPECTROSCOPY, Vol: 75, Pages: 250-258, ISSN: 0003-7028

Journal article

Solovyeva EV, Lu H, Khripoun GA, Mikhelson KN, Kazarian SGet al., 2021, In situ ATR-FTIR spectroscopic imaging of PVC, plasticizer and water in solvent-polymeric ion-selective membrane containing Cd2+-selective neutral ionophore, JOURNAL OF MEMBRANE SCIENCE, Vol: 619, ISSN: 0376-7388

Journal article

Gieroba B, Przekora A, Kalisz G, Kazimierczak P, Song CL, Wojcik M, Ginalska G, Kazarian SG, Sroka-Bartnicka Aet al., 2021, Collagen maturity and mineralization in mesenchymal stem cells cultured on the hydroxyapatite-based bone scaffold analyzed by ATR-FTIR spectroscopic imaging, MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, Vol: 119, ISSN: 0928-4931

Journal article

Kazarian SG, 2021, Perspectives on infrared spectroscopic imaging from cancer diagnostics to process analysis, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Vol: 251, ISSN: 1386-1425

Journal article

Chrabaszcz K, Kaminska K, Song CL, Morikawa J, Kujdowicz M, Michalczyk E, Smeda M, Stojak M, Jasztal A, Kazarian SG, Malek Ket al., 2021, Fourier Transform Infrared Polarization Contrast Imaging Recognizes Proteins Degradation in Lungs upon Metastasis from Breast Cancer, CANCERS, Vol: 13

Journal article

Lu H, Kazarian SG, 2020, How does high-pressure CO2 affect the morphology of PCL/PLA blends? Visualization of phase separation using in situ ATR-FTIR spectroscopic imaging, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Vol: 243, ISSN: 1386-1425

Journal article

Possenti E, Colombo C, Realini M, Song CL, Kazarian SGet al., 2020, Insight into the effects of moisture and layer build-up on the formation of lead soaps using micro-ATR-FTIR spectroscopic imaging of complex painted stratigraphies, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol: 413, Pages: 455-467, ISSN: 1618-2642

Journal article

Tiernan H, Byrne B, Kazarian SG, 2020, ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol: 241, Pages: 1-11, ISSN: 1386-1425

Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive technique that can be applied to a vast range of biological applications, from imaging cancer tissues and live cells, to determining protein content and protein secondary structure composition. This review summarises the recent advances in applications of ATR-FTIR spectroscopy to biopharmaceuticals, the application of this technique to biosimilars, and the current uses of FTIR spectroscopy in biopharmaceutical production. We discuss the use of ATR-FTIR spectroscopic imaging to investigate biopharmaceuticals, and finally, give an outlook on the possible future developments and applications of ATR-FTIR spectroscopy and spectroscopic imaging to this field. Throughout the review comparisons will be made between FTIR spectroscopy and alternative analytical techniques, and areas will be identified where FTIR spectroscopy could perhaps offer a better alternative in future studies. This review focuses on the most recent advances in the field of using ATR-FTIR spectroscopy and spectroscopic imaging to characterise and evaluate biopharmaceuticals, both in industrial and academic research based environments.

Journal article

Lu H, Kazarian SG, Sato H, 2020, Simultaneous Visualization of Phase Separation and Crystallization in PHB/PLLA Blends with In Situ ATR-FTIR Spectroscopic Imaging, MACROMOLECULES, Vol: 53, Pages: 9074-9085, ISSN: 0024-9297

Journal article

Shalygin AS, Nesterov NS, Prikhod'ko SA, Adonin NY, Martyanov ON, Kazarian SGet al., 2020, Interactions of CO2 with the homologous series of CnMIMBF4 ionic liquids studied in situ ATR-FTIR spectroscopy: spectral characteristics, thermodynamic parameters and their correlation, JOURNAL OF MOLECULAR LIQUIDS, Vol: 315, ISSN: 0167-7322

Journal article

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