Publications
381 results found
Chrabaszcz K, Kaminska K, Song CL, et al., 2021, Fourier Transform Infrared Polarization Contrast Imaging Recognizes Proteins Degradation in Lungs upon Metastasis from Breast Cancer, CANCERS, Vol: 13
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- Citations: 9
Possenti E, Colombo C, Realini M, et al., 2021, Insight into the effects of moisture and layer build-up on the formation of lead soaps using micro-ATR-FTIR spectroscopic imaging of complex painted stratigraphies, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol: 413, Pages: 455-467, ISSN: 1618-2642
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- Citations: 16
Lu H, Kazarian SG, 2020, How does high-pressure CO<sub>2</sub> affect the morphology of PCL/PLA blends? Visualization of phase separation using in situ ATR-FTIR spectroscopic imaging, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Vol: 243, ISSN: 1386-1425
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- Citations: 10
Tiernan H, Byrne B, Kazarian SG, 2020, ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol: 241, Pages: 1-11, ISSN: 1386-1425
Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive technique that can be applied to a vast range of biological applications, from imaging cancer tissues and live cells, to determining protein content and protein secondary structure composition. This review summarises the recent advances in applications of ATR-FTIR spectroscopy to biopharmaceuticals, the application of this technique to biosimilars, and the current uses of FTIR spectroscopy in biopharmaceutical production. We discuss the use of ATR-FTIR spectroscopic imaging to investigate biopharmaceuticals, and finally, give an outlook on the possible future developments and applications of ATR-FTIR spectroscopy and spectroscopic imaging to this field. Throughout the review comparisons will be made between FTIR spectroscopy and alternative analytical techniques, and areas will be identified where FTIR spectroscopy could perhaps offer a better alternative in future studies. This review focuses on the most recent advances in the field of using ATR-FTIR spectroscopy and spectroscopic imaging to characterise and evaluate biopharmaceuticals, both in industrial and academic research based environments.
Lu H, Kazarian SG, Sato H, 2020, Simultaneous Visualization of Phase Separation and Crystallization in PHB/PLLA Blends with In Situ ATR-FTIR Spectroscopic Imaging, MACROMOLECULES, Vol: 53, Pages: 9074-9085, ISSN: 0024-9297
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- Citations: 14
Shalygin AS, Nesterov NS, Prikhod'ko SA, et al., 2020, Interactions of CO<sub>2</sub> with the homologous series of C<sub>n</sub>MIMBF<sub>4</sub> ionic liquids studied in situ ATR-FTIR spectroscopy: spectral characteristics, thermodynamic parameters and their correlation, JOURNAL OF MOLECULAR LIQUIDS, Vol: 315, ISSN: 0167-7322
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- Citations: 11
Panchenko VN, Kostyukov AI, Shabalin AY, et al., 2020, New Insight into Titanium-Magnesium Ziegler-Natta Catalysts Using Photoluminescence Spectroscopy, APPLIED SPECTROSCOPY, Vol: 74, Pages: 1209-1218, ISSN: 0003-7028
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- Citations: 4
Song CL, Kazarian SG, 2020, Effect of Controlled Humidity and Tissue Hydration on Colon Cancer Diagnostic via FTIR Spectroscopic Imaging, ANALYTICAL CHEMISTRY, Vol: 92, Pages: 9691-9698, ISSN: 0003-2700
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- Citations: 11
Chan KLA, Altharawi A, Fale P, et al., 2020, Transmission Fourier Transform Infrared Spectroscopic Imaging, Mapping, and Synchrotron Scanning Microscopy with Zinc Sulfide Hemispheres on Living Mammalian Cells at Sub-Cellular Resolution, APPLIED SPECTROSCOPY, Vol: 74, Pages: 544-552, ISSN: 0003-7028
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- Citations: 13
Tiernan H, Byrne B, Kazarian SG, 2020, Insight into Heterogeneous Distribution of Protein Aggregates at the Surface Layer Using Attenuated Total Reflection-Fourier Transform Infrared Spectroscopic Imaging, ANALYTICAL CHEMISTRY, Vol: 92, Pages: 4760-4764, ISSN: 0003-2700
Song CL, Kazarian SG, 2020, Micro ATR-FTIR spectroscopic imaging of colon biopsies with a large area Ge crystal, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Vol: 228, ISSN: 1386-1425
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- Citations: 14
Kharintsev SS, Kharitonov AV, Gazizov AR, et al., 2020, Disordered Nonlinear Metalens for Raman Spectral Nanoimaging, ACS APPLIED MATERIALS & INTERFACES, Vol: 12, Pages: 3862-3872, ISSN: 1944-8244
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- Citations: 11
Byrne B, Beattie JW, Song CL, et al., 2020, ATR-FTIR spectroscopy and spectroscopic imaging of proteins, Vibrational Spectroscopy in Protein Research: from Purified Proteins to Aggregates and Assemblies, Pages: 1-22, ISBN: 9780128186114
This chapter describes applications of attenuated total reflection (ATR)–Fourier transform infrared (FTIR) spectroscopy and ATR-FTIR spectroscopic imaging to studies of proteins. The latter method combines ATR-FTIR spectroscopy with an infrared array detector for obtaining both spatial and chemical information from protein samples. Two imaging modes, micro- and macro-ATR, provide a range of imaging fields of view and spatial resolutions. Micro-ATR-FTIR imaging has been successfully used to study hanging drop protein crystallization with high spatial resolution imaging, while macro-ATR-FTIR imaging provided new opportunities for in situ studies of protein crystallization and aggregation, as well as the effect of different wettability surface properties on protein adsorption and crystallization. Recent pioneering applications of ATR-FTIR spectroscopy involve analysis of protein ligand denaturation on chromatography columns, critical for improved industrial purification of biotherapeutics. These and other new high-throughput chemical imaging approaches are discussed in this chapter.
Iruretagoyena D, Bikane K, Sunny N, et al., 2020, Enhanced selective adsorption desulfurization on CO2 and steam treated activated carbons: Equilibria and kinetics, Chemical Engineering Journal, Vol: 379, Pages: 1-11, ISSN: 1385-8947
Activated carbons (ACs) show great potential for selective adsorption removal of sulfur (SARS) from hydrocarbon fuels but require improvements in uptake and selectivity. Moreover, systematic equilibria and kinetic analyses of ACs for desulfurization are still lacking. This work examines the influence of modifying a commercial-grade activated carbon (AC) by CO2 and steam treatment for the selective adsorption removal of dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) at 323 K. An untreated AC and a charcoal Norit carbon (CN) were used for comparative purposes. Physicochemical characterization of the samples was carried out by combining N2-physisorption, X-ray diffractometry, microscopy, thermogravimetric and infrared analyses. The steam and CO2 treated ACs exhibited higher sulfur uptakes than the untreated AC and CN samples. The steam treated AC appears to be especially effective to remove sulfur, showing a remarkable sulfur uptake (~24 mgS·gads−1 from a mixture of 1500 ppmw of DBT and 1500 ppm 4,6-DMDBT) due to an increased surface area and microporosity. The modified ACs showed similar capacities for both DBT and the sterically hindered 4,6-DMDBT molecules. In addition, they were found to be selective in the presence of sulfur-free aromatics and showed good multicycle stability. Compared to other adsorbents, the modified ACs exhibited relatively high adsorption capacities. The combination of batch and fixed bed measurements revealed that the adsorption sites of the samples are characterized as heterogeneous due to the better fit to the Freundlich isotherm. The kinetic breakthrough profiles were described by the linear driving force (LDF) model.
Song CL, Vardaki MZ, Goldin RD, et al., 2019, Fourier transform infrared spectroscopic imaging of colon tissues: evaluating the significance of amide I and C-H stretching bands in diagnostic applications with machine learning, Analytical and Bioanalytical Chemistry, Vol: 411, Pages: 6969-6981, ISSN: 1618-2642
Fourier transform infrared (FTIR) spectroscopic imaging of colon biopsy tissues in transmission combined with machine learning for the classification of different stages of colon malignancy was carried out in this study. Two different approaches, an optical and a computational one, were applied for the elimination of the scattering background during the measurements and compared with the results of the machine learning model without correction for the scattering. Several different data processing pathways were implemented in order to obtain a high accuracy of the prediction model. This study demonstrates, for the first time, that C–H stretching and amide I bands are of little to no significance in the classification of the colon malignancy, based on the Gini importance values by random forest (RF). The best prediction outcome is found when supervised RF classification was carried out in the fingerprint region of the spectral data between 1500 and 1000 cm−1 (excluding the contribution of amide I and II bands). An overall prediction accuracy higher than 90% is achieved through the RF. The results also show that dysplastic and hyperplastic tissues are well distinguished. This leads to the insight that the important differences between hyperplastic and dysplastic colon tissues lie within the fingerprint region of FTIR spectra. In this study, computational correction performed better than optical correction, but the findings show that the disease states of colon biopsies can be distinguished effectively without elimination of Mie scattering effect.
Shalygin AS, Kozhevnikov I, Kazarian SG, et al., 2019, Spectroscopic imaging of deposition of asphaltenes from crude oil under flow, JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING, Vol: 181, ISSN: 0920-4105
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- Citations: 17
Kharintsev SS, Kharitonov AV, Alekseev AM, et al., 2019, Superresolution stimulated Raman scattering microscopy using 2-ENZ nano-composites., Nanoscale
Superlensing plays a crucial role in near- and far-field optical imaging with sub-wavelength resolution. One of the ways to expand optical bandwidth is surface plasmon resonances in layered metal-dielectric nanostructures. These resonances are commonly excited at a tunable single frequency. In this study, we propose the concept of a multimode far-field superlens made of a titanium oxynitride (TiON) thin film, that is a disordered metal-dielectric refractory nano-composite. These films exhibit a double epsilon-near-zero (2-ENZ) behavior near the percolation threshold and, therefore, favor super-coupling the incident laser light to surface plasmon resonances, not using such couplers as a prism, a grating, etc. We experimentally observe stimulated Raman gain emission from nano-structured TiON thin films exposed to low-power continuous-wave laser light. It is shown that superresolution of <λ/80 (near-field) and <λ/8 (far-field) is achieved due to both the enhanced third-order optical nonlinearity and the multiplicative nature of four-wave mixing. The multimode tunable far-field superlens will impact emerging diffraction-free far-field optical microscopy, random Raman lasing on meta-atoms and broadband thermophotovoltaics.
Hikima Y, Morikawa J, Kazarian SG, 2019, Analysis of molecular orientation in polymeric spherulite using polarized micro attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic imaging, Analytica Chimica Acta, ISSN: 0003-2670
© 2019 Elsevier B.V. Micro ATR-FTIR spectroscopic imaging enables the visualization of two-dimensional chemical distribution at a higher spatial resolution than macro-transmission FTIR imaging approach. In this study, micro ATR-FTIR imaging was applied for analysis of a specific morphology in a spherulite of poly(3-hydroxybutyrate) (PHB). The PHB spherulites crystallized at an isothermal condition, showed the fine band structure due to the twisting lamellar crystals during the spherulite growth under the polarized optical microscope (POM). In addition, the band structure observed in the PHB spherulite was the double band pattern in which the higher and lower birefringence banded areas alternatively appear due to the three-dimensional orientation of crystallographic axes and the biaxial refractive index ellipsoid of PHB crystalline structure. Micro ATR-FTIR spectroscopic imaging was employed for detecting the double band structure in the PHB spherulite. However, the obtained spectral images did not indicate any band structures. To detect the difference of molecular orientation among the double band structures, the micro ATR-FTIR imaging was performed with a linear polarizer at four different angles. The mean values of absorbance in each measured area changed depending on the polarizer angle. The in-plane molecular orientation to the tangential direction of spherulite, caused by the dependence of the average absorbance on the polarizer angles, was determined by the position of measured area in the spherulite and the linear dicroism of each of the spectral band used. To visualize the small difference of molecular orientation in the double band structure, micro ATR-FTIR images of the dichroic differences at three spectral bands were calculated from two different sets of polarizer angles. The micro ATR-FTIR images representing the dichroic differences displayed their corresponding distributions among three spectral bands. The complementary distributions of the dichr
Song CL, Kazarian SG, 2019, Three-dimensional depth profiling of prostate tissue by micro ATR-FTIR spectroscopic imaging with variable angles of incidence., Analyst
The depth of penetration and effective thickness in ATR-FTIR spectroscopic imaging are dependent on the wavelength and angle of incidence of the incoming light beam. We have demonstrated, for the first time, that variable angle micro ATR-FTIR, which is created via the insertion of circular apertures, is intrinsic at examining embedded components within a prostate tissue specimen. This is done by constructing a 3D model from the stacks of 2D chemical images obtained, each of which represents the spatial distribution of a chosen spectral band assigned to the component of interest at a different probing depth. ATR-FTIR imaging is also shown to have the ability to resolve subcellular components of cells such as organelles. For differentiation of diseased and non-diseased tissues, statistical tests are employed to analyse the spectral datasets obtained. When the second derivative of the spectral datasets was subjected to t-test analysis, the spectral differences between both samples in the fingerprint region are shown to be more significant at a shallow depth of penetration, with the greatest variance at the spectral band of 1235 cm-1 (vasPO2-), depicted by plotting the scores of PCA on its first two PCs. Overall, this paper demonstrates a non-destructive, label-free approach for examining heterogeneous biological samples in the z-direction to construct a 3D model using micro ATR-FTIR imaging, in a qualitative and semi-quantitative manner.
Song CL, Ryu M, Morikawa J, et al., 2018, Thermal effect on dispersive infrared spectroscopic imaging of prostate cancer tissue, JOURNAL OF BIOPHOTONICS, Vol: 11, ISSN: 1864-063X
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- Citations: 5
Kharintsev SS, Gazizov AR, Salakhov MK, et al., 2018, Near-field depolarization of tip-enhanced Raman scattering by single azo-chromophores, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 20, Pages: 24088-24098, ISSN: 1463-9076
Vaqas B, Short M, Patel I, et al., 2018, INTRAOPERATIVE RAMAN SPECTROSCOPY IDENTIFIES KEY MUTATIONS IN HUMAN GLIOMA: A NEW PLATFORM FOR PRECISION MEDICINE, Meeting of the British-Neuro-Oncology-Society (BNOS), Publisher: OXFORD UNIV PRESS INC, Pages: 353-353, ISSN: 1522-8517
Lizonova D, Muzik J, Soltys M, et al., 2018, Molecular-level insight into hot-melt loading and drug release from mesoporous silica carriers, EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, Vol: 130, Pages: 327-335, ISSN: 0939-6411
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- Citations: 14
Vichi A, Eliazyan G, Kazarian SG, 2018, Study of the Degradation and Conservation of Historical Leather Book Covers with Macro Attenuated Total Reflection-Fourier Transform Infrared Spectroscopic Imaging, ACS OMEGA, Vol: 3, Pages: 7150-7157, ISSN: 2470-1343
Ewing AV, Kazarian SG, 2018, Recent advances in the applications of vibrational spectroscopic imaging and mapping to pharmaceutical formulations, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, Vol: 197, Pages: 10-29, ISSN: 1386-1425
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- Citations: 47
Angelini G, Gasbarri C, Kazarian SG, 2018, Pluronic L121, BMIM BF4 and PEG-400 comparison to identify the best solvent for CO2 sorption, JOURNAL OF MOLECULAR LIQUIDS, Vol: 258, Pages: 85-88, ISSN: 0167-7322
Kazarian SG, 2018, Understanding Molecular Interactions with Advanced FT-IR Spectroscopy and Imaging, SPECTROSCOPY, Vol: 33, Pages: 24-26, ISSN: 0887-6703
Baker MJ, Byrne HJ, Chalmers J, et al., 2018, Clinical applications of infrared and Raman spectroscopy: state of play and future challenges, ANALYST, Vol: 143, Pages: 1735-1757, ISSN: 0003-2654
Baker MJ, Byrne HJ, Chalmers J, et al., 2018, Clinical applications of infrared and Raman spectroscopy: state of play and future challenges (vol 143, pg 1934, 2018), ANALYST, Vol: 143, Pages: 1934-1934, ISSN: 0003-2654
Ewing AV, Kazarian SG, 2018, Current trends and opportunities for the applications of in situ vibrational spectroscopy to investigate the supercritical fluid processing of polymers, JOURNAL OF SUPERCRITICAL FLUIDS, Vol: 134, Pages: 88-95, ISSN: 0896-8446
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