9 results found
Khurshid S, Saridakis E, Govada L, et al., 2014, Porous nucleating agents for protein crystallization, Nature Protocols, Vol: 9, Pages: 1621-1633, ISSN: 1750-2799
Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months.
Kertis F, Khurshid S, Okman O, et al., 2012, Heterogeneous nucleation of protein crystals using nanoporous gold nucleants, JOURNAL OF MATERIALS CHEMISTRY, Vol: 22, Pages: 21928-21934, ISSN: 0959-9428
Saridakis E, Khurshid S, Govada L, et al., 2011, Protein crystallization facilitated by molecularly imprinted polymers, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 11081-11086, ISSN: 0027-8424
Garcia-Caballero A, Gavira JA, Pineda-Molina E, et al., 2011, Optimization of Protein Crystallization: The OptiCryst Project, CRYSTAL GROWTH & DESIGN, Vol: 11, Pages: 2112-2121, ISSN: 1528-7483
Khurshid S, Haire LF, Chayen NE, 2010, Automated seeding for the optimization of crystal quality, JOURNAL OF APPLIED CRYSTALLOGRAPHY, Vol: 43, Pages: 752-756, ISSN: 0021-8898
Kertis F, Snyder J, Govada L, et al., 2010, Structure/Processing Relationships in the Fabrication of Nanoporous Gold, JOM, Vol: 62, Pages: 50-56, ISSN: 1047-4838
Fisher SJ, Helliwell JR, Khurshid S, et al., 2008, An investigation into the protonation states of the C1 domain of cardiac myosin-binding protein C, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 64, Pages: 658-664, ISSN: 2059-7983
Kuhrshid S, Govada L, Chayen NE, 2007, Dynamic Screening Experiments to Maximize Hits for Crystallization, Crystal Growth & Design, Vol: 7, Pages: 2171-2175
Khurshid S, Chayen NE, 2006, Upside-down protein crystallization - Designing microbatch experiments for microgravity, Conference on Interdisciplinary Transport Phenomena in Microgravity and Space Sciences IV, Publisher: WILEY-BLACKWELL, Pages: 208-213, ISSN: 0077-8923
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