Imperial College London


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9 results found

Khurshid S, Saridakis E, Govada L, Chayen NEet al., 2014, Porous nucleating agents for protein crystallization, Nature Protocols, Vol: 9, Pages: 1621-1633, ISSN: 1750-2799

Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months.

Journal article

Kertis F, Khurshid S, Okman O, Kysar JW, Govada L, Chayen N, Erlebacher Jet al., 2012, Heterogeneous nucleation of protein crystals using nanoporous gold nucleants, JOURNAL OF MATERIALS CHEMISTRY, Vol: 22, Pages: 21928-21934, ISSN: 0959-9428

Journal article

Saridakis E, Khurshid S, Govada L, Phan Q, Hawkins D, Crichlow GV, Lolis E, Reddy SM, Chayen NEet al., 2011, Protein crystallization facilitated by molecularly imprinted polymers, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 11081-11086, ISSN: 0027-8424

Journal article

Garcia-Caballero A, Gavira JA, Pineda-Molina E, Chayen NE, Govada L, Khurshid S, Saridakis E, Boudjemline A, Swann MJ, Stewart PS, Briggs RA, Kolek SA, Oberthuer D, Dierks K, Betzel C, Santana M, Hobbs JR, Thaw P, Savill TJ, Mesters JR, Hilgenfeld R, Bonander N, Bill RMet al., 2011, Optimization of Protein Crystallization: The OptiCryst Project, CRYSTAL GROWTH & DESIGN, Vol: 11, Pages: 2112-2121, ISSN: 1528-7483

Journal article

Khurshid S, Haire LF, Chayen NE, 2010, Automated seeding for the optimization of crystal quality, JOURNAL OF APPLIED CRYSTALLOGRAPHY, Vol: 43, Pages: 752-756, ISSN: 0021-8898

Journal article

Kertis F, Snyder J, Govada L, Khurshid S, Chayen N, Erlebacher Jet al., 2010, Structure/Processing Relationships in the Fabrication of Nanoporous Gold, JOM, Vol: 62, Pages: 50-56, ISSN: 1047-4838

Journal article

Fisher SJ, Helliwell JR, Khurshid S, Govada L, Redwood C, Squire JM, Chayen NEet al., 2008, An investigation into the protonation states of the C1 domain of cardiac myosin-binding protein C, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 64, Pages: 658-664, ISSN: 2059-7983

Journal article

Kuhrshid S, Govada L, Chayen NE, 2007, Dynamic Screening Experiments to Maximize Hits for Crystallization, Crystal Growth & Design, Vol: 7, Pages: 2171-2175

Journal article

Khurshid S, Chayen NE, 2006, Upside-down protein crystallization - Designing microbatch experiments for microgravity, Conference on Interdisciplinary Transport Phenomena in Microgravity and Space Sciences IV, Publisher: WILEY-BLACKWELL, Pages: 208-213, ISSN: 0077-8923

Conference paper

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