Publications
434 results found
Price CL, Hassi HO, English NR, et al., 2010, Methylglyoxal modulates immune responses: relevance to diabetes, Journal of Cellular and Molecular Medicine, Vol: 14, Pages: 1806-1815, ISSN: 1582-1838
Increased methylglyoxal (MG) concentrations and formation of advanced glycation end-products (AGEs) are major pathways of glycaemic damage in diabetes, leading to vascular and neuronal complications. Diabetes patients also suffer increased susceptibility to many common infections, the underlying causes of which remain elusive. We hypothesized that immune glycation damage may account for this increased susceptibility. We previously showed that the reaction mixture (RM) for MG glycation of peptide blocks up regulation of CD83 in myeloid cells and inhibits primary stimulation of T cells. Here, we continue to investigate immune glycation damage, assessing surface and intracellular cytokine protein expression by flow cytometry, T-cell proliferation using a carboxyfluorescein succinimidyl ester assay, and mRNA levels by RT-PCR. We show that the immunomodulatory component of this RM was MG itself, with MG alone causing equivalent block of CD83 and loss of primary stimulation. Block of CD83 expression could be reversed by MG scavenger N-acetyl cysteine. Further, MG within RM inhibited stimulated production of interleukin (IL)-10 protein from myeloid cells plus interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha from T cells. Loss of IL-10 and IFN-gamma was confirmed by RT-PCR analysis of mRNA, while TNF-alpha message was raised. Loss of TNF-alpha protein was also shown by ELISA of culture supernatants. In addition, MG reduced major histocompatibility complex (MHC) class I expression on the surface of myeloid cells and increased their propensity to apoptose. We conclude that MG is a potent suppressor of myeloid and T-cell immune function and may be a major player in diabetes-associated susceptibility to infection.
Murugananthan AU, Ordiz DB, Tee CT, et al., 2010, Loss of TLR 2 and 4 on Heal Plasmacytoid Dendritic Cells in Post-Operative Crohn's Disease Patients, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S746-S746, ISSN: 0016-5085
Benjamin JL, Hedin CRH, Koutsoumpas A, et al., 2010, NO CLINICAL BENEFIT OF PREBIOTICS IN THE TREATMENT OF ACTIVE CROHN'S DISEASE: A DOUBLE-BLIND, RANDOMISED, PLACEBO-CONTROLLED TRIAL, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A1-A2, ISSN: 0017-5749
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- Citations: 1
Benjamin JL, Koutsoumpas A, Hedin CRH, et al., 2010, DEMOGRAPHIC, CLINICAL AND GENETIC FACTORS ASSOCIATED WITH DYSBIOSIS IN PATIENTS WITH ACTIVE CROHN'S DISEASE, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A5-A5, ISSN: 0017-5749
Murugananthan AU, Bernardo DO, Tee CT, et al., 2010, LOSS OF TLR 2 AND 4 ON ILEAL PLASMACYTOID DENDRITIC CELLS IN POST-OPERATIVE CROHN'S DISEASE PATIENTS, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A63-A63, ISSN: 0017-5749
Milestone AN, Bernado-Ordiz D, English NR, et al., 2010, THE IMMUNOMODULATORY EFFECTS OF VITAMIN D ON LOW-DENSITY CELLS AND MONOCYTE-DERIVED DENDRITIC CELLS FROM HUMAN PERIPHERAL BLOOD IN VITRO, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A62-A63, ISSN: 0017-5749
Cox K, Knight SC, Ahmad R, et al., 2010, In patients with early breast cancer, populations of immature, cytokine producing plasmacytoid dendritic cells (PDC) decrease in tumour draining lymph nodes and express TLR9, European Breast Cancer Conference, Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: 109-109, ISSN: 1359-6349
Ng SC, Plamondon S, Al-Hassi HO, et al., 2009, A novel population of human CD56+human leucocyte antigen D-related (HLA-DR plus ) colonic lamina propria cells is associated with inflammation in ulcerative colitis, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol: 158, Pages: 205-218, ISSN: 0009-9104
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- Citations: 19
Price CL, Knight SC, 2009, Methylglyoxal: possible link between hyperglycaemia and immune suppression?, TRENDS IN ENDOCRINOLOGY AND METABOLISM, Vol: 20, Pages: 312-317, ISSN: 1043-2760
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- Citations: 37
Mann ER, Al-Hassi HO, Clark SK, et al., 2009, Imprinting of Lymphocyte Homing to Skin and Gut By Human Tissue DC, Digestive Disease Week/110th Annual Meeting of the American-Gastroenterological-Association, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: A408-A408, ISSN: 0016-5085
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- Citations: 1
Mann ER, McCarthy NE, Milestone AN, et al., 2009, LOSS OF HUMAN GAMMA DELTA T-CELLS IN CROHN'S DISEASE AND ULCERATIVE COLITIS, Annual Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A61-A61, ISSN: 0017-5749
Ng S, Hart AL, Kamm MA, et al., 2009, Mechanisms of action of probiotic bacteria – recent advances.
Al-Hassi HO, Price CL, Hart AL, et al., 2008, The expression of ILT3 upregulated on DC from inflamed mucosa: role for probiotic bacteria, Annual Congress of the British-Society-of-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 110-110, ISSN: 0019-2805
Mann ER, McCarthy NE, Hart AL, et al., 2008, Distinct gut and skin homing phenotypes of human dendritic cells and T-cells, Annual Congress of the British-Society-of-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 22-23, ISSN: 0019-2805
Mann ER, McCarthy NE, Hart AL, et al., 2008, Loss of human γδ T-cells in Crohn's disease and ulcerative colitis, Annual Congress of the British-Society-of-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 104-104, ISSN: 0019-2805
Al-Hassi HO, Cox K, Ahmad R, et al., 2008, Adoptive immunotherapy based on targeting dendritic cells in draining lymph nodes in breast cancer?, Annual Congress of the British-Society-of-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 128-128, ISSN: 0019-2805
Gellatly NL, Knight SC, Stagg AJ, 2008, Dendritic cell (DC) imprinting of homing markers on human T-cells is modulated by bacteria, Annual Congress of the British-Society-of-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 23-23, ISSN: 0019-2805
Bedford PA, Burke F, Stagg AJ, et al., 2008, Dendritic cells derived from bone marrow cells fail to acquire and present major histocompatibility complex antigens from other dendritic cells, IMMUNOLOGY, Vol: 124, Pages: 542-552, ISSN: 0019-2805
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- Citations: 5
Holden NJ, Bedford PA, McCarthy NE, et al., 2008, Dendritic cells from control but not atopic donors respond to contact and respiratory sensitizer treatment <i>in vitro</i> with differential cytokine production and altered stimulatory capacity, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 38, Pages: 1148-1159, ISSN: 0954-7894
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- Citations: 13
Knight SC, English NR, Blakemore AI, 2008, Interactions between dendritic cells, probiotic bacteria and the adipokine leptin., Proc Nutr Soc, Vol: 67, Pages: E15-E15, ISSN: 0029-6651
Knight SC, 2008, Specialized perinodal fat fuels and fashions immunity, IMMUNITY, Vol: 28, Pages: 135-138, ISSN: 1074-7613
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- Citations: 24
Ng SC, Plamondon S, Hart AL, et al., 2008, Effective probiotic treatment (VSL# 3), but not placebo, in acute ulcerative colitis is associated with down-regulation of inflammatory intestinal dendritic cells, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A37-A37, ISSN: 0017-5749
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- Citations: 5
Koutsoumpas A, Hedin CRH, Ng SC, et al., 2008, Smokers with active crohn's disease have differences in the concentration and proportion of key bacterial groups of the faecal microbiota, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A152-A153, ISSN: 0017-5749
Hedin CRH, Koutsoumpas A, Forbes A, et al., 2008, Factors that limit recruitment to clinical trials in crohn's disease, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A118-A118, ISSN: 0017-5749
Kingston R, Burke F, Robinson JH, et al., 2007, The fraction 1 and V protein antigens of <i>Yersinia pestis</i> activate dendritic cells to induce primary T cell responses, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol: 149, Pages: 561-569, ISSN: 0009-9104
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- Citations: 16
Ng SC, Plamondon S, Knight SC, et al., 2007, Accumulation of plasmacytoid dendritic cells in the intestine of acute ulcerative colitis, Annual Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A116-A116, ISSN: 0017-5749
McCarthy NE, Jones HA, Marks NA, et al., 2007, Inhaled allergen-driven CD1c up-regulation and enhanced antigen uptake by activated human respiratory-tract dendritic cells in atopic asthma, Clin Exp Allergy, Vol: 37, Pages: 72-82, ISSN: 0954-7894
Price CL, Knight SC, 2007, Advanced glycation: A novel outlook on atherosclerosis, CURRENT PHARMACEUTICAL DESIGN, Vol: 13, Pages: 3681-3687, ISSN: 1381-6128
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- Citations: 40
Stagg AJ, Norin KE, Midtvedt T, et al., 2007, Mesenteric dendritic cells from germ-free mice cause less T-cell stimulation but still induce alpha4beta7 integrin, Microbial Ecology in Health and Disease, Vol: 17, Pages: 171-183
Bedford PA, Todorovic V, Westcott EDA, et al., 2006, Adipose tissue of human omentum is a major source of dendritic cells, which lose MHC Class II and stimulatory function in Crohn's disease, JOURNAL OF LEUKOCYTE BIOLOGY, Vol: 80, Pages: 546-554, ISSN: 0741-5400
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- Citations: 37
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