Publications
434 results found
Bernardo D, Vallejo-Díez S, Mann ER, et al., 2012, IL-6 controls the intestinal inflammation in human ulcerative colitis and mediates the conditioning of dendritic cells towards a pro-inflammatory phenotype with increased skin-homing phenotype and skin-imprinting capacity on T-cells, European Journal of Immunology
Bernardo D, Al-Hassi HO, Mann ER, et al., 2012, T-cell proliferation and FoxP3 expression in human T-cells are dependent on T-cell density: physics of a confined space?, Human Imunology
T-cell proliferation rates in vitro depend on factors including initial T-cell number, dose of stimulus, culture time, and available physical space. The role of forkhead box P3 (FoxP3) in the identification of T cells with a regulatory phenotype remains controversial in humans. Through 5-carboxyfluorescein diacetate succinimidyl ester labeling of human T cells and subsequent culture of different numbers of T cells and antigen-presenting cells (APC), we studied proliferative T-cell responses and FoxP3 expression in divided T cells. T-cell proliferation rates depended on initial T-cell/APC numbers. Proliferation rates decreased when high initial T-cell numbers were increased. FoxP3 expression was expressed exclusively in virtually all divided T cells cultured at high T-cell densities, irrespective of their CD4 nature or cytokine content, and was coexpressed with T-bet. However, when T cells were cultured on larger surfaces or at lower initial numbers, FoxP3 expression was not induced in divided T cells, even when most of the cells had undergone cell division. FoxP3(+) T cells generated at high cell densities did not elicit a suppressive phenotype and FoxP3 expression was subsequently lost in time when the stimulus was removed. Therefore, caution should be observed in the use of FoxP3 expression to identify regulatory T cells in humans because its expression may be only a consequence of activation status in a restricted environment.
Bernardo D, Mann ER, Al-Hassi HO, et al., 2012, Human gut-specific homeostatic dendritic cells are generated from blood precursors by the gut microenvironment., Inflammatory Bowel Diseases, Vol: 7, Pages: 1275-1286
BACKGROUND: Dendritic cells (DC) dictate not only the type of T-cell immunity, but also homing patterns of T cells in mice. In humans, we characterized normal human gut DC and tested whether gut-specific homeostatic DC could be generated from blood precursors by factors in the gut microenvironment.METHODS: We characterized the phenotype and function of healthy human gut DC compared with blood and skin DC, and studied whether conditioning of blood DC in the presence of colonic biopsy supernatants (Bx-SN) induced gut-like phenotype and functions.RESULTS: Blood DC mostly expressed both gut and skin homing markers, indicating potential to migrate to both major immune surface organs, and induced multi-homing T cells. However, DC within gut or skin did not demonstrate this multi-homing phenotype, were tissue-specific, and induced tissue-specific T cells. Human gut DC were less stimulatory for allogeneic T cells than their dermal and blood counterparts. Human blood DC cultured in vitro lost homing marker expression. Conditioning of human enriched blood DC with colonic Bx-SN from healthy controls induced a gut-homing phenotype and a homeostatic profile. Moreover, Bx-SN-conditioned DC demonstrated a restricted T-cell stimulatory capacity and preferentially induced gut-specific T cells. Retinoic acid and transforming growth factor beta (TGF-β) mediated the acquisition of the gut-homing and homeostatic properties, respectively, induced by colonic Bx-SN on blood enriched DC.CONCLUSIONS: Tissue-specific factors manipulate immunity via modulating characteristics of DC and may provide tools to generate tissue-specific immunotherapy. (Inflamm Bowel Dis 2011;).
Peake STC, Bernardo D, Mann ER, et al., 2012, Mechanisms of action of anti-tumor necrosis factor alpha agents in Crohn’s disease, Inflamm Bowel Dis
Bernardo D, Peake STC, Knight SC, et al., 2012, Homing marker expression on circulating dendritic cells correlates with different phenotypes of Crohn’s disease, Journal of Crohn’s and Colitis
Mann E, Jialu Y, Bernardo D, et al., 2011, Probiotic strain <i>Lactobacillus casei</i> Shirota imprints a skin-homing profile on effector T-cells and exhibits dual mechanisms of immunoregulation, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 194-194, ISSN: 0019-2805
Mann E, English N, Bernardo D, et al., 2011, Protective subunit of <i>Bacillus anthracis</i> enhances human dendritic cell activation, reduces dendritic cell production of anti-inflammatory cytokines and enhances T-cell stimulation, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 209-209, ISSN: 0019-2805
Bernardo D, Vallejo-Diez S, Mann ER, et al., 2011, Intestinal homeostatic signals are lost in affected areas of ulcerative colitis patients inducing an abnormal skin homing phenotype in dendritic cells and T-cells they stimulate, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 144-144, ISSN: 0019-2805
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- Citations: 1
Bernardo D, Al-Hassi HO, Mann ER, et al., 2011, FoxP3 expression in human stimulated T-cells is transient and dependent on T-cell density -still a valid marker for identification of human regulatory T-cells?, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 196-196, ISSN: 0019-2805
Ng SC, Benjamin JL, McCarthy NE, et al., 2011, Relationship Between Human Intestinal Dendritic Cells, Gut Microbiota, and Disease Activity in Crohn's Disease, INFLAMMATORY BOWEL DISEASES, Vol: 17, Pages: 2027-2037, ISSN: 1078-0998
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- Citations: 76
Benjamin JL, Hedin CRH, Koutsoumpas A, et al., 2011, Smokers with active Crohn’s disease have a clinically relevant dysbiosis of the gastrointestinal microbiota.
Benjamin JL, Hedin CRH, Koutsoumpas A, et al., 2011, Randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active Crohn's disease, GUT, Vol: 60, Pages: 923-929, ISSN: 0017-5749
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- Citations: 234
Rahbour G, Hart AL, Al-Hassi HO, et al., 2011, Prospective study of immunological factors in non-inflammatory bowel disease enterocutaneous fistulas, BMC SURGERY, Vol: 11, ISSN: 1471-2482
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- Citations: 2
Murugananthan AU, Ordiz DB, Tozer P, et al., 2011, TH1/TH17 Profiles in Crohn's Disease: A Cross Sectional Single Centre Study in Postoperative Crohn's Disease, Conference on Digestive Disease Week 2011, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S840-S840, ISSN: 0016-5085
Murugananthan AU, Arebi N, Ordiz DB, et al., 2011, Increase in Dendritic Cell Migration Markers CCR7 and CCR9 in the Neo-Terminal Ileum of Postoperative Crohn's Disease: An Adaptive Response to Bacterial Exposure?, Conference on Digestive Disease Week 2011, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S493-S493, ISSN: 0016-5085
Tee CT, Bernardo D, Murugananthan AU, et al., 2011, UNRAVELLING THE IMMUNOMODULATORY FUNCTIONS OF GLUCAGON LIKE PEPTIDE-2 THROUGH DENDRITIC CELLS, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, Pages: A108-A108, ISSN: 0017-5749
Murugananthan AU, Arebi N, Bernardo D, et al., 2011, INCREASE IN DENDRITIC CELL MIGRATION MARKERS CCR7 AND CCR9 IN THE NEO-TERMINAL ILEUM OF POSTOPERATIVE CROHN'S DISEASE: AN ADAPTIVE RESPONSE TO BACTERIAL EXPOSURE?, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, ISSN: 0017-5749
Tozer P, Al-Hassi OH, Rayment N, et al., 2011, DENDRITIC CELL HOMING AND IMMUNE CELL FUNCTION IN CROHN'S ANAL FISTULAE, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, ISSN: 0017-5749
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- Citations: 2
Tozer P, Rayment N, Al-Hassi OH, et al., 2011, THE RECTAL MUCOSA IN PATIENTS WITH CROHN'S ANAL FISTULAE HARBOURS LOWER NUMBERS OF BIFIDOBACTERIA, AND THE FISTULA TRACTS ARE DEVOID OF A MICROBIAL ECOSYSTEM, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, Pages: A221-A221, ISSN: 0017-5749
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- Citations: 1
Murugananthan AU, Bernardo D, Tozer P, et al., 2011, TH1/TH17 PROFILES IN CROHN'S DISEASE: A CROSS SECTIONAL SINGLE CENTRE STUDY IN POSTOPERATIVE CROHN'S DISEASE, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, Pages: A212-A212, ISSN: 0017-5749
Xiang M, Al-Hassi HO, Harbige LS, et al., 2011, Gene expression of delta-6-desaturase in peripheral blood mononuclear cells, β7<SUP>+</SUP> and β7<SUP>-</SUP> lymphocytes in healthy controls and patients with Crohn's disease, PROCEEDINGS OF THE NUTRITION SOCIETY, Vol: 70, Pages: E44-E44, ISSN: 0029-6651
Mann ER, Bernardo D, Al-Hassi HO, et al., 2010, Probiotic bacteria <i>Lactobacillus casei Shirota</i> modulates human dendritic cell phenotype and function, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 129-129, ISSN: 0019-2805
Bernardo D, Al-Hassi HO, Mann ER, et al., 2010, Monocyte-derived dendritic cells lack the expression of homing markers although retinoic acid generates functional gut-homing dendritic cells, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 88-89, ISSN: 0019-2805
Mann ER, Bernardo D, Al-Hassi HO, et al., 2010, Human dendritic cell function is dependent on tissue of residence, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 40-40, ISSN: 0019-2805
Al-Hassi HO, Murugananthan A, Bernardo DO, et al., 2010, CCR7-induced homing of human gut dendritic cells mediated by LepRb; differences between ileum and colon and in Crohn's disease, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 161-162, ISSN: 0019-2805
Hart AL, Ng SC, Mann E, et al., 2010, Homing of Immune Cells: Role in Homeostasis and Intestinal Inflammation, INFLAMMATORY BOWEL DISEASES, Vol: 16, Pages: 1969-1977, ISSN: 1078-0998
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- Citations: 66
Ng SC, Kamm MA, Stagg AJ, et al., 2010, Intestinal Dendritic Cells: Their Role in Bacterial Recognition, Lymphocyte Homing, and Intestinal Inflammation, INFLAMMATORY BOWEL DISEASES, Vol: 16, Pages: 1787-1807, ISSN: 1078-0998
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- Citations: 47
Ng SC, Plamondon S, Kamm MA, et al., 2010, Immunosuppressive Effects via Human Intestinal Dendritic Cells of Probiotic Bacteria and Steroids in the Treatment of Acute Ulcerative Colitis, INFLAMMATORY BOWEL DISEASES, Vol: 16, Pages: 1286-1298, ISSN: 1078-0998
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- Citations: 94
Herber DL, Cao W, Nefedova Y, et al., 2010, Lipid accumulation and dendritic cell dysfunction in cancer, Nature Medicine, Vol: 16, Pages: 880-886, ISSN: 1546-170X
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