Imperial College London
Alternate

ProfessorStellaKnight

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 8869 3494s.knight Website

 
 
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Assistant

 

Ms Alison Scoggins +44 (0)20 8869 3534

 
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Location

 

7W032Northwick ParkNorthwick Park and St Marks Site

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Summary

 

Publications

Citation

BibTex format

@article{Landy:2015:10.1038/srep12955,
author = {Landy, J and Walker, AW and Li, JV and Al-Hassi, HO and Ronde, E and English, NR and Mann, ER and Bernardo, D and McLaughlin, SD and Parkhill, J and Ciclitira, PJ and Clark, SK and Knight, SC and Hart, AL},
doi = {10.1038/srep12955},
journal = {Scientific Reports},
title = {Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis},
url = {http://dx.doi.org/10.1038/srep12955},
volume = {5},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Faecal microbiota transplantation (FMT) is effective in the treatment of Clostridium difficile infection, where efficacy correlates with changes in microbiota diversity and composition. The effects of FMT on recipient microbiota in inflammatory bowel diseases (IBD) remain unclear. We assessed the effects of FMT on microbiota composition and function, mucosal immune response, and clinical outcome in patients with chronic pouchitis. Eight patients with chronic pouchitis (current PDAI ≥7) were treated with FMT via nasogastric administration. Clinical activity was assessed before and four weeks following FMT. Faecal coliform antibiotic sensitivities were analysed, and changes in pouch faecal and mucosal microbiota assessed by 16S rRNA gene pyrosequencing and 1H NMR spectroscopy. Lamina propria dendritic cell phenotype and cytokine profiles were assessed by flow cytometric analysis and multiplex assay. Following FMT, there were variable shifts in faecal and mucosal microbiota composition and, in some patients, changes in proportional abundance of species suggestive of a “healthier” pouch microbiota. However, there were no significant FMT-induced metabolic or immunological changes, or beneficial clinical response. Given the lack of clinical response following FMT via a single nasogastric administration our results suggest that FMT/bacteriotherapy for pouchitis patients requires further optimisation.
AU  - Landy,J
AU  - Walker,AW
AU  - Li,JV
AU  - Al-Hassi,HO
AU  - Ronde,E
AU  - English,NR
AU  - Mann,ER
AU  - Bernardo,D
AU  - McLaughlin,SD
AU  - Parkhill,J
AU  - Ciclitira,PJ
AU  - Clark,SK
AU  - Knight,SC
AU  - Hart,AL
DO  - 10.1038/srep12955
PY  - 2015///
SN  - 2045-2322
TI  - Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep12955
UR  - http://hdl.handle.net/10044/1/27320
VL  - 5
ER  -
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