Imperial College London
Alternate

ProfessorStellaKnight

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 8869 3494s.knight Website

 
 
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Assistant

 

Ms Alison Scoggins +44 (0)20 8869 3534

 
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Location

 

7W032Northwick ParkNorthwick Park and St Marks Site

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Summary

 

Publications

Citation

BibTex format

@article{Comino:2016:10.3402/fnr.v60.30324,
author = {Comino, I and Bernardo, D and Bancel, E and de, Lourdes Moreno M and Sánchez, B and Barro, F and uligoj, T and Ciclitira, PJ and Cebolla, Á and Knight, SC and Branlard, G and Sousa, C},
doi = {10.3402/fnr.v60.30324},
journal = {Food & Nutrition Research},
title = {Identification and molecular characterization of oat peptides implicated on coeliac immune response},
url = {http://dx.doi.org/10.3402/fnr.v60.30324},
volume = {60},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: Oats provide important nutritional and pharmacological properties,although their safety in coeliac patients remains controversial. Previous studies haveconfirmed that the reactivity of the anti-33-mer monoclonal antibody with different oatvarieties is proportional to the immune responses in terms of T-cell proliferation.Although the impact of these varieties on the adaptive response has been studied, therole of the dendritic cells is still poorly understood. The aim of this study is tocharacterize different oat fractions and to study their effect on dendritic cells fromcoeliac patients.Methods and results: Protein fractions were isolated from oat grains and analysed bySDS-PAGE. Several proteins were characterized in the prolamin fraction usingimmunological and proteomic tools, and by Nano-LC-MS/MS. These proteins,analogous to α- and γ-gliadin-like, showed reactive sequences to anti-33-mer antibodysuggesting their immunogenic potential. That was further confirmed as some of thenewly identified oat peptides had a differential stimulatory capacity on circulatingdendritic cells from coeliac patients compared with healthy controls.Conclusions :This is the first time, to our knowledge, where newly identified oatpeptides have been shown to elicit a differential stimulatory capacity on circulatingdendritic cells obtained from coeliac patients, potential identifying immunogenicproperties of these oat peptides.
AU  - Comino,I
AU  - Bernardo,D
AU  - Bancel,E
AU  - de,Lourdes Moreno M
AU  - Sánchez,B
AU  - Barro,F
AU  - uligoj,T
AU  - Ciclitira,PJ
AU  - Cebolla,Á
AU  - Knight,SC
AU  - Branlard,G
AU  - Sousa,C
DO  - 10.3402/fnr.v60.30324
PY  - 2016///
SN  - 1654-6628
TI  - Identification and molecular characterization of oat peptides implicated on coeliac immune response
T2  - Food & Nutrition Research
UR  - http://dx.doi.org/10.3402/fnr.v60.30324
UR  - http://hdl.handle.net/10044/1/28118
VL  - 60
ER  -
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