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@article{Metcalf:2016:10.1021/acs.analchem.6b01594,
author = {Metcalf, GAD and Shibakawa, A and Patel, H and Sita-Lumsden, A and Zivi, A and Rama, N and Bevan, C and Ladame, S},
doi = {10.1021/acs.analchem.6b01594},
journal = {Analytical Chemistry},
pages = {8091--8098},
title = {Amplification-free detection of circulating microRNA biomarkers from body fluids based on fluorogenic oligonucleotide-templated reaction between engineered peptide nucleic acid probes: application to prostate cancer diagnosis},
url = {http://dx.doi.org/10.1021/acs.analchem.6b01594},
volume = {88},
year = {2016}
}

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TY  - JOUR
AB  - Highly abundant in cells, microRNAs (or miRs) play a key role as regulators of gene expression. A proportion of them are also detectable in biofluids making them ideal noninvasive biomarkers for pathologies in which miR levels are aberrantly expressed, such as cancer. Peptide nucleic acids (PNAs) are engineered uncharged oligonucleotide analogues capable of hybridizing to complementary nucleic acids with high affinity and high specificity. Herein, novel PNA-based fluorogenic biosensors have been designed and synthesized that target miR biomarkers for prostate cancer (PCa). The sensing strategy is based on oligonucleotide-templated reactions where the only miR of interest serves as a matrix to catalyze an otherwise highly unfavorable fluorogenic reaction. Validated in vitro using synthetic RNAs, these newly developed biosensors were then shown to detect endogenous concentrations of miR in human blood samples without the need for any amplification step and with minimal sample processing. This low-cost, quantitative, and versatile sensing technology has been technically validated using gold-standard RT-qPCR. Compared to RT-qPCR however, this enzyme-free, isothermal blood test is amenable to incorporation into low-cost portable devices and could therefore be suitable for widespread public screening.
AU  - Metcalf,GAD
AU  - Shibakawa,A
AU  - Patel,H
AU  - Sita-Lumsden,A
AU  - Zivi,A
AU  - Rama,N
AU  - Bevan,C
AU  - Ladame,S
DO  - 10.1021/acs.analchem.6b01594
EP  - 8098
PY  - 2016///
SN  - 0003-2700
SP  - 8091
TI  - Amplification-free detection of circulating microRNA biomarkers from body fluids based on fluorogenic oligonucleotide-templated reaction between engineered peptide nucleic acid probes: application to prostate cancer diagnosis
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.6b01594
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.6b01594
UR  - http://hdl.handle.net/10044/1/38819
VL  - 88
ER  -

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