Imperial College London
Alternate

DR LADAME

Faculty of Engineering, Department of Bioengineering

Reader in Biosensor Development
 
 
 
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Contact

 

+44 (0)20 7594 5308s.ladame Website

 
 
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Location

 

U321ASir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Al:2017:10.1002/anie.201701356,
author = {Al, Sulaiman D and Chang, JYH and Ladame, S},
doi = {10.1002/anie.201701356},
journal = {Angewandte Chemie - International Edition},
pages = {5247--5251},
title = {Subnanomolar detection of oligonucleotides via templated fluorogenic reaction in hydrogels: controlling diffusion to improve sensitivity},
url = {http://dx.doi.org/10.1002/anie.201701356},
volume = {56},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Oligonucleotide-templated reactions are valuable tools for nucleic acid sensing both invitro and invivo. They are typically carried out under conditions that make any reaction in the absence of template highly unfavorable (most commonly by using a low concentration of reactants), which has a negative impact on the detection sensitivity. Herein, we report a novel platform for fluorogenic oligonucleotide-templated reactions between peptide nucleic acid probes embedded within permeable agarose and alginate hydrogels. We demonstrate that under conditions of restricted mobility (that is, limited diffusion), non-specific interactions between probes are prevented, thus leading to lower background signals. When applied to nucleic acid sensing, this accounts for a significant increase in sensitivity (that is, lower limit of detection). Optical nucleic acid sensors based on fluorogenic peptide nucleic acid probes embedded in permeable, physically crosslinked, alginate beads were also engineered and proved capable of detecting DNA concentrations as low as 100pm.
AU  - Al,Sulaiman D
AU  - Chang,JYH
AU  - Ladame,S
DO  - 10.1002/anie.201701356
EP  - 5251
PY  - 2017///
SN  - 1433-7851
SP  - 5247
TI  - Subnanomolar detection of oligonucleotides via templated fluorogenic reaction in hydrogels: controlling diffusion to improve sensitivity
T2  - Angewandte Chemie - International Edition
UR  - http://dx.doi.org/10.1002/anie.201701356
UR  - http://hdl.handle.net/10044/1/45452
VL  - 56
ER  -
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