Imperial College London
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Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{McQuail:2020:10.1074/jbc.RA120.014107,
author = {McQuail, J and Switzer, A and Burchell, L and Wigneshweraraj, S},
doi = {10.1074/jbc.RA120.014107},
journal = {Journal of Biological Chemistry},
pages = {12355--12367},
title = {The RNA-binding protein Hfq assembles into foci-like structures in nitrogen starved Escherichia coli},
url = {http://dx.doi.org/10.1074/jbc.RA120.014107},
volume = {295},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The initial adaptive responses to nutrient depletion in bacteria often occur at the level of gene expression. Hfq is an RNA-binding protein present in diverse bacterial lineages and contributes to many different aspects of RNA metabolism during gene expression. Using photoactivated localization microscopy (PALM) and single molecule tracking, we demonstrate that Hfq forms a distinct and reversible focus-like structure in Escherichia coli specifically experiencing long-term nitrogen (N) starvation. Using the ability of T7 phage to replicate in N-starved bacteria as a biological probe of E. coli cell function during N starvation, we demonstrate that Hfq foci have a role in the adaptive response of E. coli to long-term N starvation. We further show that Hfq foci formation does not depend on gene expression once N starvation has set in and occurs independently of the transcription factor N-regulatory protein C (NtrC), that activates the initial adaptive response to N starvation in E. coli These results serve as a paradigm to demonstrate that bacterial adaptation to long-term nutrient starvation can be spatiotemporally coordinated and can occur independently of de novo gene expression during starvation.
AU  - McQuail,J
AU  - Switzer,A
AU  - Burchell,L
AU  - Wigneshweraraj,S
DO  - 10.1074/jbc.RA120.014107
EP  - 12367
PY  - 2020///
SN  - 0021-9258
SP  - 12355
TI  - The RNA-binding protein Hfq assembles into foci-like structures in nitrogen starved Escherichia coli
T2  - Journal of Biological Chemistry
UR  - http://dx.doi.org/10.1074/jbc.RA120.014107
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32532816
UR  - https://www.jbc.org/content/295/35/12355
UR  - http://hdl.handle.net/10044/1/80992
VL  - 295
ER  -
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