Imperial College London
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Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2021:10.3389/fmicb.2021.692512,
author = {Wang, Z and Wang, H and Mulvenna, N and Sanz-Hernandez, M and Zhang, P and Li, Y and Ma, J and Wang, Y and Matthews, S and Wigneshweraraj, S and Liu, B},
doi = {10.3389/fmicb.2021.692512},
journal = {Frontiers in Microbiology},
pages = {1--10},
title = {A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase},
url = {http://dx.doi.org/10.3389/fmicb.2021.692512},
volume = {12},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of Bacillus subtilis, employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase via the β and β′ subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts.
AU  - Wang,Z
AU  - Wang,H
AU  - Mulvenna,N
AU  - Sanz-Hernandez,M
AU  - Zhang,P
AU  - Li,Y
AU  - Ma,J
AU  - Wang,Y
AU  - Matthews,S
AU  - Wigneshweraraj,S
AU  - Liu,B
DO  - 10.3389/fmicb.2021.692512
EP  - 10
PY  - 2021///
SN  - 1664-302X
SP  - 1
TI  - A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2021.692512
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000661654100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2021.692512/full
UR  - http://hdl.handle.net/10044/1/103068
VL  - 12
ER  -
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