Imperial College London
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Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Brown:2016:10.1016/j.jmb.2016.08.004,
author = {Brown, DR and Sheppard, CM and Matthews, S and Wigneshweraraj, S},
doi = {10.1016/j.jmb.2016.08.004},
journal = {Journal of Molecular Biology},
pages = {3911--3919},
title = {The Xp10 bacteriophage protein P7 inhibits transcription by the major and major variant forms of the host RNA polymerase via a common mechanism},
url = {http://dx.doi.org/10.1016/j.jmb.2016.08.004},
volume = {428},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The σ factor is a functionally obligatory subunit of the bacterial transcription machinery, the RNA polymerase. Bacteriophage-encoded small proteins that either modulate or inhibit the bacterial RNAP to allow the temporal regulation of bacteriophage gene expression often target the activity of the major bacterial σ factor, σ70. Previously, we showed that during Xanthomonas oryzae phage Xp10 infection, the phage protein P7 inhibits the host RNAP by preventing the productive engagement with the promoter and simultaneously displaces the σ70 factor from the RNAP. In this study, we demonstrate that P7 also inhibits the productive engagement of the bacterial RNAP containing the major variant bacterial σ factor, σ54, with its cognate promoter. The results suggest for the first time that the major variant form of the host RNAP can also be targeted by bacteriophage-encoded transcription regulatory proteins. Since the major and major variant σ factor interacting surfaces in the RNAP substantially overlap, but different regions of σ70 and σ54 are used for binding to the RNAP, our results further underscore the importance of the σ–RNAP interface in bacterial RNAP function and regulation and potentially for intervention by antibacterials.
AU  - Brown,DR
AU  - Sheppard,CM
AU  - Matthews,S
AU  - Wigneshweraraj,S
DO  - 10.1016/j.jmb.2016.08.004
EP  - 3919
PY  - 2016///
SN  - 1089-8638
SP  - 3911
TI  - The Xp10 bacteriophage protein P7 inhibits transcription by the major and major variant forms of the host RNA polymerase via a common mechanism
T2  - Journal of Molecular Biology
UR  - http://dx.doi.org/10.1016/j.jmb.2016.08.004
UR  - http://hdl.handle.net/10044/1/38891
VL  - 428
ER  -
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