Imperial College London
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Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2018:10.1002/chem.201800401,
author = {Liu, B and Wang, Z and Lan, L and Yang, Q and Zhang, P and Shi, L and Lang, Y and Tabib-Salazar, A and Wigneshweraraj, S and Zhang, J and Wang, Y and Tang, Y and Matthews, S and Zhang, X},
doi = {10.1002/chem.201800401},
journal = {Chemistry - A European Journal},
pages = {6727--6731},
title = {A rapid colorimetric method to visualize protein interactions},
url = {http://dx.doi.org/10.1002/chem.201800401},
volume = {24},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - As key molecules in most biological pathways, proteins physically contact one or more biomolecules in a highly specific manner. Several driving forces (i.e., electrostatic and hydrophobic) facilitate such interactions and a variety of methods have been developed to monitor these processes both in vivo and in vitro. In this work, a new method is reported for the detection of protein interactions by visualizing a color change of a cyanine compound, a supramolecule complex of 3,3-di-(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MTC). Nuclear magnetic resonance (NMR) studies suggest that the hydrophobic nature of the protein surfaces drives MTC into different types of aggregates with distinct colors. When proteins interact with other biomolecules, the hydrophobic surface of the complex differs, resulting in a shift in the form of MTC aggregation, which results in a color change. As a result, this in vitro method has the potential to become a rapid tool for the confirmation of protein-biomolecule interactions, without the requirements for sophisticated instrumentation or approaches.
AU  - Liu,B
AU  - Wang,Z
AU  - Lan,L
AU  - Yang,Q
AU  - Zhang,P
AU  - Shi,L
AU  - Lang,Y
AU  - Tabib-Salazar,A
AU  - Wigneshweraraj,S
AU  - Zhang,J
AU  - Wang,Y
AU  - Tang,Y
AU  - Matthews,S
AU  - Zhang,X
DO  - 10.1002/chem.201800401
EP  - 6731
PY  - 2018///
SN  - 0947-6539
SP  - 6727
TI  - A rapid colorimetric method to visualize protein interactions
T2  - Chemistry - A European Journal
UR  - http://dx.doi.org/10.1002/chem.201800401
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29505156
UR  - http://hdl.handle.net/10044/1/58698
VL  - 24
ER  -
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