Imperial College London
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DrSaravanaRamasamy

Faculty of Medicine, National Heart & Lung Institute

Advanced Research Fellow
 
 
 
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Contact

 

+44 (0)20 3313 1182s.ramasamy Website

 
 
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Location

 

CRB 2003Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Biswas:2021:10.1101/2021.01.20.427385,
author = {Biswas, L and Chen, J and De, Angelis J and Chatzis, A and Nanchahal, J and Dustin, ML and Ramasamy, SK and Kusumbe, AP},
doi = {10.1101/2021.01.20.427385},
title = {SUMIC: A Simple Ultrafast Multicolor Immunolabelling and Clearing Approach for Whole-Organ and Large Tissue 3D Imaging},
url = {http://dx.doi.org/10.1101/2021.01.20.427385},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>High-resolution whole-organ imaging of cleared tissues captures cellular and molecular insights within the intact tissue and tumour microenvironments. However, current immunolabelling and clearing methods are complicated and time-consuming; extending to several weeks. Here, we developed <jats:bold><jats:underline>S</jats:underline></jats:bold>imple <jats:bold><jats:underline>U</jats:underline></jats:bold>ltrafast <jats:bold><jats:underline>M</jats:underline></jats:bold>ulticolor <jats:bold><jats:underline>I</jats:underline></jats:bold>mmunolabelling and <jats:bold><jats:underline>C</jats:underline></jats:bold>learing or <jats:bold>SUMIC</jats:bold>, a method that enables multicolor immunolabelling and clearing of whole murine organs and human tissues within 2 to 2.5 days. Moreover, SUMIC is simple, robust, non-hazardous and versatile comprising antigen retrieval, permeabilization, collagenase-based digestion, immunolabelling, dehydration, and clearing. SUMIC permits quantitative and singlecell resolution analysis and detection of rare cells in whole organs, for example, round αSMA positive cells in the thymus. Upon volumetric imaging, SUMIC-processed samples retain normal tissue architecture and can be used for paraffin-embedding and histology. We employed the SUMIC method for whole-organ mapping of lymphatic vessels across different ages and organs. This analysis revealed the expansion of lymphatic vessels in endocrine tissues but not in any other organs with aging. Hence, SUMIC will accelerate discoveries compared to other whole organ imaging pipelines.</jats:p>
AU  - Biswas,L
AU  - Chen,J
AU  - De,Angelis J
AU  - Chatzis,A
AU  - Nanchahal,J
AU  - Dustin,ML
AU  - Ramasamy,SK
AU  - Kusumbe,AP
DO  - 10.1101/2021.01.20.427385
PY  - 2021///
TI  - SUMIC: A Simple Ultrafast Multicolor Immunolabelling and Clearing Approach for Whole-Organ and Large Tissue 3D Imaging
UR  - http://dx.doi.org/10.1101/2021.01.20.427385
ER  -
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