Imperial College London
Alternate

ProfessorSaraRankin

Faculty of Medicine, National Heart & Lung Institute

Professor of Leukocyte and Stem Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 3172s.rankin

 
 
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Assistant

 

Ms Georgina Moss +44 (0)20 7594 2151

 
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Location

 

Office no. 351Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pillay:2020:10.1002/JLB.1MA0420-571RR,
author = {Pillay, J and Tregay, N and Juzenaite, G and Carlin, LM and Pirillo, C and Gaboriau, DCA and Farahi, N and Summers, C and Lo, Celso C and Chilvers, ER and Rankin, S and De, Filippo K},
doi = {10.1002/JLB.1MA0420-571RR},
journal = {Journal of Leukocyte Biology},
pages = {1175--1185},
title = {Effect of the CXCR4 antagonist plerixafor on endogenous neutrophil dynamics in the bone marrow, lung and spleen},
url = {http://dx.doi.org/10.1002/JLB.1MA0420-571RR},
volume = {107},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Treatment with the CXCR4 antagonist, plerixafor (AMD3100), has been proposed for clinical use in patients with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome and in pulmonary fibrosis. However, there is controversy with respect to the impact of plerixafor on neutrophil dynamics in the lung, which may affect its safety profile. In this study, we investigated the kinetics of endogenous neutrophils by direct imaging, using confocal intravital microscopy in mouse bone marrow, spleen, and lungs. Neutrophils are observed increasing their velocity and exiting the bone marrow following plerixafor administration, with a concomitant increase in neutrophil numbers in the blood and spleen, while the marginated pool of neutrophils in the lung microvasculature remained unchanged in terms of numbers and cell velocity. Use of autologous radiolabeled neutrophils and SPECT/CT imaging in healthy volunteers showed that plerixafor did not affect GMCSFprimed neutrophil entrapment or release in the lungs. Taken together, these data suggest that plerixafor causes neutrophil mobilization from the bone marrow but does not impact on lung marginated neutrophil dynamics and thus is unlikely to compromise respiratory host defense both in humans and mice.
AU  - Pillay,J
AU  - Tregay,N
AU  - Juzenaite,G
AU  - Carlin,LM
AU  - Pirillo,C
AU  - Gaboriau,DCA
AU  - Farahi,N
AU  - Summers,C
AU  - Lo,Celso C
AU  - Chilvers,ER
AU  - Rankin,S
AU  - De,Filippo K
DO  - 10.1002/JLB.1MA0420-571RR
EP  - 1185
PY  - 2020///
SN  - 0741-5400
SP  - 1175
TI  - Effect of the CXCR4 antagonist plerixafor on endogenous neutrophil dynamics in the bone marrow, lung and spleen
T2  - Journal of Leukocyte Biology
UR  - http://dx.doi.org/10.1002/JLB.1MA0420-571RR
UR  - https://jlb.onlinelibrary.wiley.com/doi/full/10.1002/JLB.1MA0420-571RR
UR  - http://hdl.handle.net/10044/1/79588
VL  - 107
ER  -
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