Imperial College London
Alternate

ProfessorSaraRankin

Faculty of Medicine, National Heart & Lung Institute

Professor of Leukocyte and Stem Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 3172s.rankin

 
 
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Assistant

 

Ms Georgina Moss +44 (0)20 7594 2151

 
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Location

 

Office no. 351Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ferreira:2021:10.1016/j.msec.2020.111393,
author = {Ferreira, SA and Young, G and Jones, JR and Rankin, S},
doi = {10.1016/j.msec.2020.111393},
journal = {Materials Science and Engineering: C},
pages = {1--13},
title = {Bioglass/carbonate apatite/collagen composite scaffold dissolution products promote human osteoblast differentiation},
url = {http://dx.doi.org/10.1016/j.msec.2020.111393},
volume = {118},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - OssiMend® Bioactive (Collagen Matrix Inc., NJ) is a three-component porous composite bone graft device of 45S5 Bioglass/carbonate apatite/collagen. Our in vitro studies showed that conditioned media of the dissolution products of OssiMend Bioactive stimulated primary human osteoblasts to form mineralized bone-like nodules in vitro in one week, in basal culture media (no osteogenic supplements). Osteoblast differentiation was followed by gene expression analysis and a mineralization assay. In contrast, the dissolution products from commercial OssiMend (Bioglass-free carbonate apatite/collagen scaffolds), or from 45S5 Bioglass particulate alone, did not induce the mineralization of the extracellular matrix, but did induce osteoblast differentiation to mature osteoblasts, evidenced by the strong upregulation of BGLAP and IBSP mRNA levels. The calcium ions and soluble silicon species released from 45S5 Bioglass particles and additional phosphorus release from OssiMend mediated the osteostimulatory effects. Medium conditioned with OssiMend Bioactive dissolution had a much higher concentration of phosphorus and silicon than media conditioned with OssiMend and 45S5 Bioglass alone. While OssiMend and OssiMend Bioactive led to calcium precipitation in cell culture media, OssiMend Bioactive produced a higher concentration of soluble silicon than 45S5 Bioglass and higher dissolution of phosphorus than OssiMend. These in vitro results suggest that adding 45S5 Bioglass to OssiMend produces a synergistic osteostimulation effect on primary human osteoblasts.
AU  - Ferreira,SA
AU  - Young,G
AU  - Jones,JR
AU  - Rankin,S
DO  - 10.1016/j.msec.2020.111393
EP  - 13
PY  - 2021///
SN  - 0928-4931
SP  - 1
TI  - Bioglass/carbonate apatite/collagen composite scaffold dissolution products promote human osteoblast differentiation
T2  - Materials Science and Engineering: C
UR  - http://dx.doi.org/10.1016/j.msec.2020.111393
UR  - https://www.sciencedirect.com/science/article/pii/S0928493120333117?via%3Dihub
UR  - http://hdl.handle.net/10044/1/82444
VL  - 118
ER  -
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