Imperial College London
Alternate

ProfessorSunilShaunak

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor in Infectious Diseases
 
 
 
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Contact

 

s.shaunak Website

 
 
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Location

 

8.N15Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Monnery:2017:10.1016/j.ijpharm.2017.02.048,
author = {Monnery, BD and Wright, M and Cavill, R and Hoogenboom, R and Shaunak, S and Steinke, JHG and Thanou, M},
doi = {10.1016/j.ijpharm.2017.02.048},
journal = {International Journal of Pharmaceutics},
pages = {249--258},
title = {Cytotoxicity of polycations: Relationship of molecular weight and the hydrolytic theory of the mechanism of toxicity},
url = {http://dx.doi.org/10.1016/j.ijpharm.2017.02.048},
volume = {521},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The mechanism of polycation cytotoxicity and the relationship to polymer molecular weight is poorly understood. To gain an insight into this important phenomenon a range of newly synthesised uniform (near monodisperse) linear polyethylenimines, commercially available poly(l-lysine)s and two commonly used PEI-based transfectants (broad 22 kDa linear and 25 kDa branched) were tested for their cytotoxicity against the A549 human lung carcinoma cell line. Cell membrane damage assays (LDH release) and cell viability assays (MTT) showed a strong relationship to dose and polymer molecular weight, and increasing incubation times revealed that even supposedly “non-toxic” low molecular weight polymers still damage cell membranes. The newly proposed mechanism of cell membrane damage is acid catalysed hydrolysis of lipidic phosphoester bonds, which was supported by observations of the hydrolysis of DOPC liposomes.
AU  - Monnery,BD
AU  - Wright,M
AU  - Cavill,R
AU  - Hoogenboom,R
AU  - Shaunak,S
AU  - Steinke,JHG
AU  - Thanou,M
DO  - 10.1016/j.ijpharm.2017.02.048
EP  - 258
PY  - 2017///
SN  - 0378-5173
SP  - 249
TI  - Cytotoxicity of polycations: Relationship of molecular weight and the hydrolytic theory of the mechanism of toxicity
T2  - International Journal of Pharmaceutics
UR  - http://dx.doi.org/10.1016/j.ijpharm.2017.02.048
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000397613700028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S0378517317301382?via%3Dihub
VL  - 521
ER  -
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