Imperial College London

DrDevSooranna

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 8846 7895s.sooranna Website

 
 
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Location

 

H.3.36Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Publication Type
Year
to

267 results found

Singh N, Herbert B, Sooranna G, Shah NM, Das A, Sooranna SR, Johnson MRet al., 2021, Is there an inflammatory stimulus to human term labour?, PLoS One, Vol: 16, Pages: 1-22, ISSN: 1932-6203

Inflammation is thought to play a pivotal role in the onset of term and some forms of preterm labour. Although, we recently found that myometrial inflammation is a consequence rather than a cause of term labour, there are several other reproductive tissues, including amnion, choriodecidua parietalis and decidua basalis, where the inflammatory stimulus to labour may occur. To investigate this, we have obtained amnion, choriodecidual parietalis and decidua basalis samples from women at various stages of pregnancy and spontaneous labour. The inflammatory cytokine profile in each tissue was determine by Bio-Plex Pro® cytokine multiplex assays and quantitative RT-PCR. Active motif assay was used to study transcription activation in the choriodecidua parietalis. Quantitative RT-PCR was use to study the pro-labour genes (PGHS-2, PGDH, OTR and CX43) in all of the tissues at the onset of labour and oxytocin (OT) mRNA expression in the choriodecidual parietalis and decidua basalis. Statistical significance was ascribed to a P value <0.05. In the amnion and choriodecidua parietalis, the mRNA levels of various cytokines decreased from preterm no labour to term no labour samples, but the protein levels were unchanged. The choriodecidua parietalis showed increase in the protein levels of IL-1β and IL-6 in the term early labour samples. In the amnion and decidua basalis, the protein levels of several cytokines rose in term established labour. The multiples of the median derived from the 19-plex cytokine assay were greater in term early labour and term established labour samples from the choriodecidua parietalis, but only in term established labour for myometrium. These data suggest that the inflammatory stimulus to labour may begin in the choriodecidua parietalis, but the absence of any change in prolabour factor mRNA levels suggests that the cytokines may act on the myometrium where we observed changes in transcription factor activation and increases in prolabour gene

Journal article

Yang F, Zhao J, Luo X, Li T, Wang Z, Wei Q, Lu H, Meng Y, Cai K, Lu L, Lu Y, Chen L, Sooranna SR, Luo L, Song J, Meng Let al., 2021, Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma, FRONTIERS IN ONCOLOGY, Vol: 11, ISSN: 2234-943X

Journal article

Yulia A, Singh N, Varley AJ, Lei K, Markovic D, Sooranna SR, Johnson MRet al., 2021, PKA and AKIP1 interact to mediate cAMP-driven COX-2 expression: A potentially pivotal interaction in preterm and term labour, PLOS ONE, Vol: 16, ISSN: 1932-6203

Journal article

Huang D-N, Wang S, Sooranna SR, Miao J-Het al., 2021, The Efficacy of Natural Bioactive Compounds for the Treatment of Naso-pharyngeal Carcinoma, MINI-REVIEWS IN MEDICINAL CHEMISTRY, Vol: 21, Pages: 1679-1691, ISSN: 1389-5575

Journal article

Liang Q, Lin Q, Li Y, Luo W, Huang X, Jiang Y, Qin C, Nong J, Chen X, Sooranna SR, Pinhu Let al., 2020, Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model, JOURNAL OF IMMUNOLOGY RESEARCH, Vol: 2020, ISSN: 2314-8861

Journal article

Liu Y, Huang D, Li B, Liu W, Sooranna SR, Pan X, Huang Z, Guo Jet al., 2020, Association between alpha 1-antitrypsin and acute coronary syndrome, EXPERIMENTAL AND THERAPEUTIC MEDICINE, Vol: 20, ISSN: 1792-0981

Journal article

Zuo Y, Lu X, Wang X, Sooranna SR, Tao L, Chen S, Li H, Huang D, Nai G, Chen H, Pan C, Huang C, Pang Yet al., 2020, High-Dose Aluminum Exposure Further Alerts Immune Phenotype in Aplastic Anemia Patients (Aug, 10.1007/s12011-020-02412-4, 2020), BIOLOGICAL TRACE ELEMENT RESEARCH, Vol: 199, Pages: 3178-3178, ISSN: 0163-4984

Journal article

Zuo Y, Lu X, Wang X, Sooranna SR, Tao L, Chen S, Li H, Huang D, Nai G, Chen H, Pan C, Huang C, Pang Yet al., 2020, High-Dose Aluminum Exposure Further Alerts Immune Phenotype in Aplastic Anemia Patients, BIOLOGICAL TRACE ELEMENT RESEARCH, Vol: 199, Pages: 1743-1753, ISSN: 0163-4984

Journal article

Singh N, Herbert B, Sooranna G, Das A, Sooranna SR, Yellon SM, Johnson MRet al., 2019, Distinct preterm labor phenotypes have unique inflammatory signatures and contraction associated protein profiles, Biology of Reproduction, Vol: 101, Pages: 1031-1045, ISSN: 0006-3363

Preterm labor (PTL) is the predominant cause of childhood morbidity and mortality. It has several phenotypes, each with a distinct etiology often involving inflammation. Here, in samples of reproductive tissues obtained in early PTL from women with phenotypically defined PTL, we examined the presence and distribution of inflammation and its relationship with prolabor gene expression. In chorioamnionitis (CA-PTL), cytokine protein concentrations were increased across all tissues; in idiopathic (I-PTL), the inflammatory changes were limited to the choriodecidua; inflammation was not a feature of placental abruption (PA-PTL). CA-PTL was associated with activation of p65 in the myometrium and AP-1 in the choriodecidua, and PA-PTL with CREB in the choriodecidua. In the myometrium, PGHS-2 mRNA level was increased in CA- and I-PTL; in the amnion, PGHS-2 mRNA level was higher in PA- and I-PTL, while in CA-PTL, OT, OTR mRNA, and CX-43 expression were increased. In the choriodecidua, PGHS-2 mRNA level was unchanged, but in CA and I-PTL, OT mRNA level were increased and OTR was reduced. These data show that CA-PTL is associated with widespread inflammation and prolabor gene expression. In contrast, in I-PTL, inflammation is limited to the choriodecidua, with discrete increases in PGHS-2 in the amnion and OT in the choriodecidua. Inflammation is not a feature of PA-PTL, which is associated with increased OT and OTR in the amnion.

Journal article

Huang Z, Liu Y, Liang L, Liu W, Sooranna SR, Mo J, Liu L, Li Z, Li K, Guo Jet al., 2019, Correlation between coronary stenosis and Toll-like receptors 2 and 4 levels in Chinese Zhuang patients with coronary heart disease, EXPERIMENTAL AND THERAPEUTIC MEDICINE, Vol: 18, Pages: 2346-2352, ISSN: 1792-0981

Journal article

Huang Z, Liu Y, Liang L, Liu W, Sooranna SR, Mo J, Liu L, Li Z, Guo Jet al., 2019, Association of Toll-like receptor 4 polymorphisms with the risk of coronary artery disease in the ethnic Zhuang population of the Guangxi Province of China, GENE, Vol: 708, Pages: 1-9, ISSN: 0378-1119

Journal article

Varley A, Yulia A, Lei K, Jayarajan V, Singh N, Sooranna SR, Johnson MRet al., 2019, Novel and Transformative Changes in the cAMP Effector Pathway in Three Distinct Types of Preterm Labour., 66th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 219A-220A, ISSN: 1933-7191

Conference paper

He Y, Li Z, Wang W, Sooranna SR, Shi Y, Chen Y, Wu C, Zeng J, Tang Q, Xie Het al., 2018, Chemical profiles and simultaneous quantification of Aurantii fructus by use of HPLC-Q-TOF-MS combined with GC-MS and HPLC methods, Molecules, Vol: 23, ISSN: 1420-3049

Aurantii fructus (AF) is a traditional Chinese medicine that has been used to improve gastrointestinal motility disorders for over a thousand years, but there is no exhaustive identification of the basic chemical components and comprehensive quality control of this herb. In this study, high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS) and gas chromatography coupled mass spectrometry (GC-MS) were employed to identify the basic chemical compounds, and high-performance liquid chromatography (HPLC) was developed to determine the major biochemical markers from AF extract. There were 104 compounds belonging to eight structure types, including 13 amino acids or peptides, seven alkaloids, 18 flavanones, 14 flavones, 15 polymethoxyflavonoids, six triterpenoids, nine coumarins, and 18 volatile oils, as well as four other compounds that were systematically identified as the basic components from AF, and among them, 41 compounds were reported for the first time. Twelve bioactive ingredients were chosen as the benchmark markers to evaluate the quality of AF. The analysis was completed with a gradient elution at a flow rate of 0.7 mL/min within 55 min. This efficient method was validated showing good linearity, precision, stability, repeatability and recovery. Furthermore, the method was successfully applied to the simultaneous determination of 12 chemical markers in different samples of AF. This study could be applied to the identification of multiple bioactive substances and improve the quality control of AF.

Journal article

Guo Y, Hua R, Bian S, Xie X, Ma J, Cai Y, Sooranna SR, Cheng Wet al., 2018, Intrauterine Bakri Balloon and Vaginal Tamponade Combined with Abdomina Compression for the Management of Postpartum Hemorrhage, JOURNAL OF OBSTETRICS AND GYNAECOLOGY CANADA, Vol: 40, Pages: 561-565, ISSN: 1701-2163

Journal article

You Y, Qin Y, Lin X, Yang F, Wang J, Yuan F, Sooranna SR, Liao Pet al., 2018, Upregulated fractalkine levels in Chinese patients with lupus nephritis, CYTOKINE, Vol: 104, Pages: 23-28, ISSN: 1043-4666

Journal article

Sassine AJ, Wang Y, Singh N, Sooranna GR, Herbert BR, Sooranna SR, Crawford MA, Johnson MRet al., 2018, Fatty Acid Composition in the Preterm and Term Human Placentae., 65th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 302A-302A, ISSN: 1933-7191

Conference paper

Singh N, Herbert B, Sooranna GR, Orsi NM, Edey L, Dasgupta T, Sooranna SR, Yellon SM, Johnson MRet al., 2017, Is myometrial inflammation a cause or a consequence of term human labour?, JOURNAL OF ENDOCRINOLOGY, Vol: 235, Pages: 69-83, ISSN: 0022-0795

Journal article

Tirlapur N, O'Dea K, Soni S, Davies R, Sooranna S, Johnson M, Wilson M, Takata Met al., 2017, Pathological Stretch Of Endothelial Cells Activates Marginated Monocytes To Release Microvesicles In An In Vitro Model Of Ventilator-Induced Lung Injury, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X

Conference paper

Liang Y, Huang X, Jiang Y, Qin Y, Peng D, Huang Y, Li J, Sooranna SR, Liao Pet al., 2017, Endothelial protein C receptor polymorphisms and risk of sepsis in a Chinese population, JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, Vol: 45, Pages: 504-513, ISSN: 0300-0605

Journal article

Yulia A, Singh N, Sooranna SR, Johnson MRet al., 2017, The role of cyclic AMP as an anti-inflammatory in human myometrium, Publisher: WILEY, Pages: 102-103, ISSN: 1470-0328

Conference paper

Das A, Sooranna S, Johnson MR, 2017, Can the Anti-Inflammatory Effect of Progesterone Be Enhanced in Stretched IL-1 beta Stimulated Human Amnion Cells?, 64th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 282A-282A, ISSN: 1933-7191

Conference paper

Sassine AJ, Sooranna GR, Singh N, Herbert BR, Sooranna SR, Crawford MA, Johnson MRet al., 2017, Placental Fatty Acid Translocase (FAT/CD36) and Tranport Protein-4 (FATP-4) Are Less Expressed in the Human Preterm Than Term Placenta., 64th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 280A-280A, ISSN: 1933-7191

Conference paper

Yulia A, Singh N, Lei K, Sooranna S, Johnson Met al., 2017, The effects of inflammation and stretch on cyclic AMP levels and pathways components in myometrial tissues, Publisher: WILEY, Pages: 52-52, ISSN: 1470-0328

Conference paper

Lei K, Georgiou EX, Yulia A, Sooranna SR, Brosens JJ, Bennett PR, Johnson MRet al., 2017, Progesterone and the Repression of Myometrial Inflammation: The Roles of MKP-1 and the AP-1 System., 64th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 107A-107A, ISSN: 1933-7191

Conference paper

Huang X, Zeng Y, Jiang Y, Qin Y, Luo W, Xiang S, Sooranna SR, Liao Pet al., 2017, Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-kappa B, MEDIATORS OF INFLAMMATION, Vol: 2017, ISSN: 0962-9351

Journal article

Hu D, Wang H, Huang X, Jiang Y, Qin Y, Xiong B, Qin G, Sooranna SR, Pinhu Let al., 2016, Investigation of association between IL-8 serum levels and IL8 polymorphisms in Chinese patients with sepsis, GENE, Vol: 594, Pages: 165-170, ISSN: 0378-1119

Journal article

Georgiou E, Lei K, Lai P, Sooranna S, Johnson Met al., 2016, Progesterone repression of IL-1 beta action is maintained in human myometrium after the onset of labour, Publisher: WILEY-BLACKWELL, Pages: E12-E13, ISSN: 1470-0328

Conference paper

Georgiou E, Lei K, Singh N, Sooranna S, Johnson Met al., 2016, Chorioamnionitis-induced preterm labour is associated with progesterone receptor cofactor protein changes, Publisher: WILEY-BLACKWELL, Pages: E10-E10, ISSN: 1470-0328

Conference paper

Jiang Y, Zeng Y, Huang X, Qin Y, Luo W, Xiang S, Sooranna SR, Liao Pet al., 2016, Nur77 attenuates endothelin-1 expression via downregulation of NF-kappa B and p38 MAPK in A549 cells and in an ARDS rat model, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 311, Pages: L1023-L1035, ISSN: 1040-0605

Journal article

Yulia A, Singh N, Lei K, Sooranna SR, Johnson MRet al., 2016, Cyclic AMP effectors regulate myometrial oxytocin receptor expression, Endocrinology, Vol: 157, Pages: 4411-4422, ISSN: 1945-7170

The factors that initiate human labor are poorly understood. We have tested the hypothesis that a decline in cAMP/protein kinase A (PKA) function leads to the onset of labor. Initially, we identified myometrial cAMP/PKA-responsive genes (six up-regulated and five down-regulated genes) and assessed their expression in myometrial samples taken from different stages of pregnancy and labor. We found that the oxytocin receptor (OTR) was one of the cAMP-repressed genes, and, given the importance of OTR in the labor process, we studied the mechanisms involved in greater detail using small interfering RNA, chemical agonists, and antagonists of the cAMP effectors. We found that cAMP-repressed genes, including OTR, increased with the onset of labor. Our in vitro studies showed that cAMP acting via PKA reduced OTR expression but that in the absence of PKA, cAMP acts via exchange protein activated by cAMP (EPAC) to increase OTR expression. In early labor myometrial samples, PKA levels and activity declined and Epac1 levels increased, perhaps accounting for the increase in myometrial OTR mRNA and protein levels at this time. In vitro exposure of myometrial cells to stretch and IL-1β increased OTR levels and reduced basal and forskolin-stimulated cAMP and PKA activity, as judged by phospho-cAMP response element-binding protein levels, but neither stretch nor IL-1β had any effect on PKA or EPAC1 levels. In summary, there is a reduction in the activity of the cAMP/PKA pathway with the onset of human labor potentially playing a critical role in regulating OTR expression and the transition from myometrial quiescence to activation.

Journal article

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