Publications
282 results found
Sassine AJ, Wang Y, Singh N, et al., 2018, Fatty Acid Composition in the Preterm and Term Human Placentae., 65th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 302A-302A, ISSN: 1933-7191
Singh N, Herbert B, Sooranna GR, et al., 2017, Is myometrial inflammation a cause or a consequence of term human labour?, JOURNAL OF ENDOCRINOLOGY, Vol: 235, Pages: 69-83, ISSN: 0022-0795
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- Citations: 33
Tirlapur N, O'Dea K, Soni S, et al., 2017, Pathological Stretch Of Endothelial Cells Activates Marginated Monocytes To Release Microvesicles In An In Vitro Model Of Ventilator-Induced Lung Injury, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Liang Y, Huang X, Jiang Y, et al., 2017, Endothelial protein C receptor polymorphisms and risk of sepsis in a Chinese population, JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, Vol: 45, Pages: 504-513, ISSN: 0300-0605
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- Citations: 4
Das A, Sooranna S, Johnson MR, 2017, Can the Anti-Inflammatory Effect of Progesterone Be Enhanced in Stretched IL-1 beta Stimulated Human Amnion Cells?, 64th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 282A-282A, ISSN: 1933-7191
Yulia A, Singh N, Sooranna SR, et al., 2017, The role of cyclic AMP as an anti-inflammatory in human myometrium, Publisher: WILEY, Pages: 102-103, ISSN: 1470-0328
Sassine AJ, Sooranna GR, Singh N, et al., 2017, Placental Fatty Acid Translocase (FAT/CD36) and Tranport Protein-4 (FATP-4) Are Less Expressed in the Human Preterm Than Term Placenta., 64th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 280A-280A, ISSN: 1933-7191
Yulia A, Singh N, Lei K, et al., 2017, The effects of inflammation and stretch on cyclic AMP levels and pathways components in myometrial tissues, Publisher: WILEY, Pages: 52-52, ISSN: 1470-0328
Lei K, Georgiou EX, Yulia A, et al., 2017, Progesterone and the Repression of Myometrial Inflammation: The Roles of MKP-1 and the AP-1 System., 64th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), Publisher: SAGE PUBLICATIONS INC, Pages: 107A-107A, ISSN: 1933-7191
Huang X, Zeng Y, Jiang Y, et al., 2017, Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB, MEDIATORS OF INFLAMMATION, Vol: 2017, ISSN: 0962-9351
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- Citations: 12
Hu D, Wang H, Huang X, et al., 2016, Investigation of association between IL-8 serum levels and <i>IL8</i> polymorphisms in Chinese patients with sepsis, GENE, Vol: 594, Pages: 165-170, ISSN: 0378-1119
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- Citations: 11
Jiang Y, Zeng Y, Huang X, et al., 2016, Nur77 attenuates endothelin-1 expression via downregulation of NF-κB and p38 MAPK in A549 cells and in an ARDS rat model, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 311, Pages: L1023-L1035, ISSN: 1040-0605
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- Citations: 34
Georgiou E, Lei K, Lai P, et al., 2016, Progesterone repression of IL-1β action is maintained in human myometrium after the onset of labour, Publisher: WILEY-BLACKWELL, Pages: E12-E13, ISSN: 1470-0328
Georgiou E, Lei K, Singh N, et al., 2016, Chorioamnionitis-induced preterm labour is associated with progesterone receptor cofactor protein changes, Publisher: WILEY-BLACKWELL, Pages: E10-E10, ISSN: 1470-0328
Yulia A, Singh N, Lei K, et al., 2016, Cyclic AMP effectors regulate myometrial oxytocin receptor expression, Endocrinology, Vol: 157, Pages: 4411-4422, ISSN: 1945-7170
The factors that initiate human labor are poorly understood. We have tested the hypothesis that a decline in cAMP/protein kinase A (PKA) function leads to the onset of labor. Initially, we identified myometrial cAMP/PKA-responsive genes (six up-regulated and five down-regulated genes) and assessed their expression in myometrial samples taken from different stages of pregnancy and labor. We found that the oxytocin receptor (OTR) was one of the cAMP-repressed genes, and, given the importance of OTR in the labor process, we studied the mechanisms involved in greater detail using small interfering RNA, chemical agonists, and antagonists of the cAMP effectors. We found that cAMP-repressed genes, including OTR, increased with the onset of labor. Our in vitro studies showed that cAMP acting via PKA reduced OTR expression but that in the absence of PKA, cAMP acts via exchange protein activated by cAMP (EPAC) to increase OTR expression. In early labor myometrial samples, PKA levels and activity declined and Epac1 levels increased, perhaps accounting for the increase in myometrial OTR mRNA and protein levels at this time. In vitro exposure of myometrial cells to stretch and IL-1β increased OTR levels and reduced basal and forskolin-stimulated cAMP and PKA activity, as judged by phospho-cAMP response element-binding protein levels, but neither stretch nor IL-1β had any effect on PKA or EPAC1 levels. In summary, there is a reduction in the activity of the cAMP/PKA pathway with the onset of human labor potentially playing a critical role in regulating OTR expression and the transition from myometrial quiescence to activation.
Xu H, van Deel E, Johnson M, et al., 2016, Pregnancy mitigates cardiac pathology in a mouse model of left ventricular pressure overload, American Journal of Physiology - Heart and Circulatory Physiology, Vol: 311, Pages: H807-H814, ISSN: 0363-6135
In western countries heart disease is the leading cause of maternal death during pregnancy. The effect of pregnancy on the heart is difficult to study in patients with preexisting heart disease. Since experimental studies are scarce, we investigated the effect of pressure-overload, produced by transverse aortic constriction (TAC) in mice, on the ability to conceive, pregnancy outcome, and maternal cardiac structure and function. Four weeks of TAC produced left ventricular (LV) hypertrophy and dysfunction with marked interstitial fibrosis, decreased capillary density and induced pathological cardiac gene expression. Pregnancy increased relative LV and right ventricular weight without affecting the deterioration of LV function following TAC. Surprisingly, the TAC-induced increase in relative heart and lung weight was mitigated by pregnancy, which was accompanied by a partial normalization of capillary density and natriuretic peptide type A expression. Additionally, the combination of pregnancy and TAC increased the cardiac phosphorylation of c-Jun, and STAT1, but reduced PI3K phosphorylation. Finally, TAC did not significantly affect conception rate, pregnancy duration, uterus size, litter size and pup weight. In conclusion, we found that, rather than exacerbating the changes associated with cardiac pressure-overload, pregnancy actually attenuated pathological LV remodeling and mitigated pulmonary congestion, capillary rarefaction and pathological gene expression produced by TAC, suggesting a positive effect of pregnancy on the pressure-overloaded heart.
Feng Y, Chen S, Li C, et al., 2016, Curettage after uterine artery embolization combined with methotrexate treatment for caesarean scar pregnancy., Experimental and Therapeutic Medicine, Vol: 12, Pages: 1469-1475, ISSN: 1792-1015
In the present study, we evaluated the diagnosis and management modalities of caesarean scar pregnancy (CSP). Thirty patients diagnosed with CSP were retrospectively studied between February, 2010 and February, 2012. Twenty-five patients were offered prophylactic uterine artery embolization (UAE) and methotrexate (MTX) prior to uterine suction curettage. Five cases were referred from other hospitals where the initial management with uterine suction curettage had resulted in uncontrollable massive haemorrhage, 4 of the cases had UAE and one proceeded immediately to hysterectomy. In the 25 patients treated with prophylactic UAE and MTX, 12 had laparoscopy-guided curettage and 13 had ultrasound-guided curettage without complication. The results showed that the 25 patients with CSP, who received prophylactic UAE and MTX prior to uterine curettage, recovered without complications. Five patients referred from other hospitals, where uterine curettage was the primary procedure, had severe complications including uncontrolled vaginal bleeding and uterine rupture. Four of the five patients were treated successfully with emergency UAE and the remaining patient underwent emergency hysterectomy as ultrasound examination detected significant haemorrhage between the uterus and the bladder. Of the 25 patients who received prophylactic UAE combined with MTX, there were no reports of irregular menstruation or serious adverse effects. Notably, the decrease in serum human chorionic gonadotropin (HCG) levels 3 days post-surgery was greater with ultrasound-guided curettage (84.3±5.5%) than with laparoscopy-guided curettage (76.3±10.2%). In summary, the data suggested that prophylactic UAE with MTX followed by ultrasound-guided curettage is the most effective therapeutic approach in CSP.
Xiang S, Zeng Y, Xiong B, et al., 2016, Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells., Journal of Inflammation, Vol: 13, ISSN: 1476-9255
BACKGROUND: Endothelin-1 (ET-1) is involved in pulmonary vascular remodeling. The aim of this study was to investigate the biochemical interactions between PPAR-γ, TGF-β1 and ET-1 in vitro. METHODS: A549 cells were pre-treated with S2505 (10 μM), S2871 (10 μM) with/without SB203580 (10 μM) for 60 min following 2 h treatment with 10 ng/mL TGF-β1. A549 cells were also transfected with positive or negative PPAR-γ plasmids for comparison. RT-PCR, ELISA, western blotting and confocal laser scanning microscopy (CLSM) were used to measure the relevant expression of mRNA, protein, mediators of pathways and nuclear factor translocation. RESULTS: SB203580 inhibited TGF-β1 induced ET-1 expression in A549 cells. S2871 decreased PPAR-γ mRNA and increase TGF-β1-induced ET-1 expression. S2871 increased phosphorylation of p38 MAPK and Smad2. Cells transfected with PPAR-γ negative plasmid increased TGF-β1 induced ET-1 expression, and increased the expression of phospho-p38 MAPK and phospho-Smad2. S2505 increased PPAR-γ mRNA expression, suppressed the increased TGF-β1-induced expression of ET-1. S2505 inhibited TGF-β1 induced phosphorylation of p38 MAPK and Smad2, also the nuclear translocation of Smad2. Cells transfected with PPAR-γ positive plasmid reduced TGF-β1-induced ET-1 expression, and inhibited the expression of phospho-p38 MAPK and phospho-Smad2. CONCLUSIONS: TGF-β1 induced release of endothelin-1 is PPAR-γ dependent in cultured A549 cells.
Tang Q, Jiang Q, Sooranna SR, et al., 2016, Effects of Electroacupuncture on Pain Threshold of Laboring Rats and the Expression of Norepinephrine Transporter and alpha 2 Adrenergic Receptor in the Central Nervous System, Evidence-based Complementary and Alternative Medicine, Vol: 2016, ISSN: 1741-427X
To observe the effects of electroacupuncture on pain threshold of laboring rats and the expression of norepinephrine transporter and α2 adrenergic receptor in the central nervous system to determine the mechanism of the analgesic effect of labor. 120 pregnant rats were divided into 6 groups: a control group, 4 electroacupuncture groups, and a meperidine group. After interventions, the warm water tail-flick test was used to observe pain threshold. NE levels in serum, NET, and α2AR mRNA and protein expression levels in the central nervous system were measured. No difference in pain threshold was observed between the 6 groups before intervention. After intervention, increased pain thresholds were observed in all groups except the control group with a higher threshold seen in the electroacupuncture groups. Serum NE levels decreased in the electroacupuncture and MP groups. Increases in NET and α2AR expression in the cerebral cortex and decreases in enlarged segments of the spinal cord were seen. Acupuncture increases uptake of NE via cerebral NET and decreases its uptake by spinal NET. The levels of α2AR are also increased and decreased, respectively, in both tissues. This results in a decrease in systemic NE levels and may be the mechanism for its analgesic effects.
Georgiou EX, Lei K, Lai PF, et al., 2016, The study of progesterone action in human myometrial explants., Molecular Human Reproduction, ISSN: 1460-2407
STUDY HYPOTHESIS: Myometrial explants represent a superior model compared with cell culture models for the study of human myometrial progesterone (P4) signalling in parturition. STUDY FINDING: Gene expression analysis showed myometrial explants closely resemble the in vivo condition and the anti-inflammatory action of P4 is not lost with labour onset. WHAT IS KNOWN ALREADY: Circulating P4 levels decline before the onset of parturition in most animals, but not in humans. This has led to the suggestion that there is a functional withdrawal of P4 action at the myometrial level prior to labour onset. However, to date, no evidence of a loss of P4 function has been provided, with studies hampered by a lack of a physiologically relevant model. STUDY DESIGN, SAMPLES/MATERIALS, METHODS: Myometrial biopsies obtained at Caesarean section were dissected into explants after a portion was immediately snap frozen (t = 0). Microarray analysis was used to compare gene expression of t = 0 with paired (i) explants, (ii) passage 4 myometrial cell cultures or (iii) the hTERT myometrial cell line. Western blotting and chemokine/cytokine assays were used to study P4 signalling in myometrial explants. MAIN RESULTS AND THE ROLE OF CHANCE: Gene expression comparison of t = 0 to the three models demonstrated that explants more closely resemble the in vivo status. At the protein level, explants maintain both P4 receptor (PR) and glucocorticoid receptor (GR) levels versus t = 0 whereas cells only maintain GR levels. Additionally, treatment with 1 µM P4 led to a reduction in interleukin-1 (IL-1) β-driven cyclooxygenase-2 in explants but not in cells. P4 signalling in explants was PR-mediated and associated with a repression of p65 and c-Jun phosphorylation. Furthermore, the anti-inflammatory action of P4 was maintained after labour onset. LIMITATIONS/REASONS FOR CAUTION: There is evidence of basal inflammation in the myometrial explant model. WIDER IMPLICATIONS OF THE FINDINGS: Myomet
Waldorf KMA, Singh N, Mohan AR, et al., 2015, Uterine overdistention induces preterm labor mediated by inflammation: observations in pregnant women and nonhuman primates, American Journal of Obstetrics and Gynecology, Vol: 213, Pages: 830.e1-830.e19, ISSN: 1097-6868
ObjectiveUterine overdistention is thought to induce preterm labor in women with twin and multiple pregnancies, but the pathophysiology remains unclear. We investigated for the first time the pathogenesis of preterm birth associated with rapid uterine distention in a pregnant nonhuman primate model.Study DesignA nonhuman primate model of uterine overdistention was created using preterm chronically catheterized pregnant pigtail macaques (Macaca nemestrina) by inflation of intraamniotic balloons (N = 6), which were compared to saline controls (N = 5). Cesarean delivery was performed due to preterm labor or at experimental end. Microarray, quantitative reverse transcriptase polymerase chain reaction, Luminex (Austin, TX), and enzyme-linked immunosorbent assay were used to measure messenger RNA (mRNA) and/or protein levels from monkey (amniotic fluid, myometrium, maternal plasma) and human (amniocytes, amnion, myometrium) tissues. Statistical analysis employed analysis of covariance and Wilcoxon rank sum. Biomechanical forces were calculated using the law of Laplace.ResultsPreterm labor occurred in 3 of 6 animals after balloon inflation and correlated with greater balloon volume and uterine wall stress. Significant elevations of inflammatory cytokines and prostaglandins occurred following uterine overdistention in an “inflammatory pulse” that correlated with preterm labor (interleukin [IL]-1β, tumor necrosis factor [TNF]-α, IL-6, IL-8, CCL2, prostaglandin E2, prostaglandin F2α, all P < .05). A similar inflammatory response was observed in amniocytes in vitro following mechanical stretch (IL1β, IL6, and IL8 mRNA multiple time points, P < .05), in amnion of women with polyhydramnios (IL6 and TNF mRNA, P < .05) and in amnion (TNF-α) and myometrium of women with twins in early labor (IL6, IL8, CCL2, all P < .05). Genes differentially expressed in the nonhuman primate after balloon inflation and in women with polyhydramnios
Lei K, Georgiou EX, Chen L, et al., 2015, Progesterone and the Repression of Myometrial Inflammation: The Roles of MKP-1 and the AP-1 System, MOLECULAR ENDOCRINOLOGY, Vol: 29, Pages: 1454-1467, ISSN: 0888-8809
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- Citations: 29
Lei K, Sooranna SR, Johnson MR, 2015, Expression data from primary culture human myometrial cells, Genomics Data, Vol: 6, Pages: 182-183, ISSN: 2213-5960
Inflammation plays a central role in many human diseases. Human parturition also resembles an inflammatory reaction, where progesterone (P4) and progesterone receptors (PRs) have already been demonstrated to suppress contraction-associated gene expression. In our previous studies, we have found that the progesterone actions, including progesterone-induced gene expression and progesterone's anti-inflammatory effect, are mediated by PR, GR or both. In this study, we used microarrays (GSE68171) to find P4 and IL-1β responsive genes and IL-1β responsive genes which were repressed by P4. These data may provide a broader view of gene networks and cellular functions regulated by P4 and IL-1β in human myometrial cells. These data will also help us understand the role of PR and GR in human parturition.
You Y, Qin Y, Lin X, et al., 2015, Methylprednisolone attenuates lipopolysaccharide-induced Fractalkine expression in kidney of Lupus-prone MRL/lpr mice through the NF-kappaB pathway, BMC Nephrology, Vol: 16, ISSN: 1471-2369
Anim-Nyame N, Gamble J, Sooranna SR, et al., 2015, Relationship between insulin resistance and tissue blood flow in preeclampsia, JOURNAL OF HYPERTENSION, Vol: 33, Pages: 1057-1063, ISSN: 0263-6352
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- Citations: 7
Yulia A, Sooranna SR, Johnson MR, 2015, The Effect of cAMP Elevating Agents on cAMP Related Genes During Gestation in Human Myometrium, REPRODUCTIVE SCIENCES, Vol: 22, Pages: 142A-142A, ISSN: 1933-7191
Georgiou EX, Lai PF, Yulia A, et al., 2015, Progesterone Repression of IL-1β-Driven COX-2 Expression in Myometrial Explant Cultures, Publisher: SAGE PUBLICATIONS INC, Pages: 134A-134A, ISSN: 1933-7191
Zheng X, Sooranna SR, Ma D, et al., 2015, The Effect of Different Fat Contents of Maternal Diets on Mice Which Suffered Perinatal Asphyxia, REPRODUCTIVE SCIENCES, Vol: 22, Pages: 365A-365A, ISSN: 1933-7191
Lei K, Sooranna SR, Johnson MR, 2015, P4 Plays Its Anti-inflammatory Role Primarily Via GR and the Inhibition of AP-1 in Human Myometrial Cells, REPRODUCTIVE SCIENCES, Vol: 22, Pages: 136A-136A, ISSN: 1933-7191
Yulia A, Georgiou EX, Lei K, et al., 2015, The Effects of Combinations of cAMP and Progesterone on Progesterone Responsive Genes in Term Human Myometrium, REPRODUCTIVE SCIENCES, Vol: 22, Pages: 142A-142A, ISSN: 1933-7191
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