Publications
282 results found
Singh N, Herbert BR, Sooranna SR, et al., 2015, Differences in Inflammatory Chemokine/Cytokine Expression Between Different Stages of Term Labour and Clinical Subtypes of Preterm Labour, REPRODUCTIVE SCIENCES, Vol: 22, Pages: 140A-140A, ISSN: 1933-7191
Das A, Sooranna SR, Johnson MR, 2015, Stretch-Induced Changes in the Human Amnion., REPRODUCTIVE SCIENCES, Vol: 22, Pages: 325A-325A, ISSN: 1933-7191
Fan X, Chen S, Qian J, et al., 2015, Incidence and Interrelated Factors in Patients With Congenital Hypothyroidism as Detected by Newborn Screening in Guangxi, China, Global Pediatric Health, Vol: 2, ISSN: 2333-794X
Background. A newborn screening program (NSP) for congenital hypothyroidism (CH) was carried out in Guangxi in order to understand the incidence of CH and the factors interrelated to major types of CH in this region of China. Methods. During 2009 to 2013, data from 930 612 newborns attending NSP in Guangxi were collected. Patients were classified with either permanent CH (PCH) or transient CH (TCH) after 2 years of progressive study. Results. A total of 1210 patients were confirmed with CH with an incidence of 1/769, including 68 PCH and 126 TCH cases with incidences of 1/6673 and 1/3385, respectively. The frequency of thyroid stimulating hormone values greater than 5 mIU/L was 7.2%, which, based on WHO guidelines, suggests that the population was mildly iodine deficient. Conclusions. The incidence of CH was high in Guangxi. Approximately two thirds of CH patients were TCH, which may be due to a deficiency in iodine within the population.
Wang H, Wei Y, Zeng Y, et al., 2014, The association of polymorphisms of <i>TLR4</i> and <i>CD14</i> genes with susceptibility to sepsis in a Chinese population, BMC MEDICAL GENETICS, Vol: 15
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- Citations: 20
Liao P, Qin Y, Xiong B, et al., 2014, Overexpression of Fas and FasL Is Associated with Infectious Complications and Severity of Experimental Severe Acute Pancreatitis by Promoting Apoptosis of Lymphocytes, INFLAMMATION, Vol: 37, Pages: 1202-1212, ISSN: 0360-3997
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- Citations: 29
Singh N, Yuiia A, Terzidou V, et al., 2014, 6.3 The Commonalities and Differences in the Molecular Expression of Different Phenotype-specific Causes of Preterm Labour in Comparison to Term Labour., Arch Dis Child Fetal Neonatal Ed, Vol: 99
: Preterm birth comprises of several distinct clinical phenotypes. The commonalities and differences of the genes altered in the different phenotypes of preterm labour (PTL) have yet to be elucidated. Myometrial biopsies were collected during caesarean section from women in preterm no labour (PNL; n = 26), preterm labour (chorioamnionitis, placental abruption, polyhydramnios and idiopathic; n = 11, 6, 16 and 4 respectively), preterm twins (NL and L; n = 12), term no labour (n = 18), term early labour (EAL, <3 cm dilatation; n = 10) and established labour (ESL, ≥3 cm dilatation; n = 12). Samples were rapidly frozen at -80(0)C. Total RNA was extracted using RNeasy kit from Qiagen and converted to cDNA. Copy numbers of PGHS-2, CXCL-8, oxytocin receptor, IL-6, connexin 43 and GAPDH were measured by qPCR using Rotor-Gene(TM) (Corbett Research, Australia). In PTL compared to PNL, PGHS-2 was significantly overall increased in all the phenotype specific groups except placental abruption (p < 0.05). PGHS-2 levels were also significantly increased in twin no labour versus PNL (p < 0.05), but there was no difference in PGHS-2 expression between twin no labour and twin labour. There was no statistical difference in the expression of PGHS-2 when the different phenotypes of PTL were compared to both EAL and ESL. There was no significant change in the expression of CXCL-8 or OTR among both the labouring and non-labouring preterm phenotypes. Connexin-43 expression did not change among the preterm groups. These data show that PGHS-2 appears to play a significant role in driving the process of preterm labour in all distinct phenotypes (except placental abruption).
Mitsuya K, Singh N, Sooranna SR, et al., 2014, Epigenetics of Human Myometrium: DNA Methylation of Genes Encoding Contraction-Associated Proteins in Term and Preterm Labor, BIOLOGY OF REPRODUCTION, Vol: 90, ISSN: 0006-3363
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- Citations: 22
Waldorf KA, Sooranna S, Gravett M, et al., 2014, Acute Uterine Stretch Induces an Inflammatory "Pulse" in Amniotic Fluid and Maternal Plasma Followed by Preterm Labor in Nonhuman Primates, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 96A-96A, ISSN: 1933-7191
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- Citations: 2
Georgiou EX, Edey LF, Lei K, et al., 2014, RU486-Induced Preterm Labour: A Shifting Paradigm?, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 141A-142A, ISSN: 1933-7191
Herbert BR, Yulia A, Sooranna SR, et al., 2014, Phosphodiesterase Inhibition in Combination with Progesterone Treatment Can Inhibit Inflammation-Induced Preterm Labour in Mice., REPRODUCTIVE SCIENCES, Vol: 21, Pages: 143A-143A, ISSN: 1933-7191
Yulia A, Singh N, Lei K, et al., 2014, Molecular Markers of cAMP Related Genes during Gestation and Parturition in Human Myometrium, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 347A-347A, ISSN: 1933-7191
Singh N, Yulia A, Lei K, et al., 2014, The Molecular Expression of Different Phenotype-Specific Causes of Preterm Labour in Comparison to Term Labour, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 340A-341A, ISSN: 1933-7191
Mitsuya K, Singh N, Sooranna SR, et al., 2014, Alterations to DNA Methylome Identified in Myometrium with Idiopathic Preterm Labor, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 96A-97A, ISSN: 1933-7191
Zheng X, Sooranna SR, Ma D, et al., 2014, Acute Expression of Cytokines in the Brain and Long Term Neurocognitive Outcome in a Perinatal Asphyxia Mouse Model, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 376A-377A, ISSN: 1933-7191
Zollner J, Ke H-Y, Sooranna SR, et al., 2014, The Expression of the Sodium Potassium ATPases in Cardiac Myocytes in a Guinea Pig Model of Transaortic Constriction, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 384A-384A, ISSN: 1933-7191
Lei K, Chen L, Georgiou EX, et al., 2014, Progesterone Antagonizes IL-1b-Induced COX-2 Expression by Inhibiting AP-1 in Human Myometrial Cells, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 243A-244A, ISSN: 1933-7191
Das A, Lei K, Sooranna SR, et al., 2014, Mechanical Stretch of Human Myometrial Cells in the Absence and Presence of Specific FAK Inhibition., REPRODUCTIVE SCIENCES, Vol: 21, Pages: 139A-140A, ISSN: 1933-7191
Yulia A, Georgiou EX, Das A, et al., 2014, Enhancement of Anti-Inflammatory Effect by the Combination of Progesterone and cAMP on IL-1b-Stimulated Human Myometrial Cells., REPRODUCTIVE SCIENCES, Vol: 21, Pages: 111A-111A, ISSN: 1933-7191
Chen L, Lei K, Malawana J, et al., 2014, Cyclic AMP enhances progesterone action in human myometrial cells, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 382, Pages: 334-343, ISSN: 0303-7207
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- Citations: 18
Bajoria R, Sooranna S, Chatterjee R, 2013, Effect of lipid composition of cationic SUV liposomes on materno-fetal transfer of warfarin across the perfused human term placenta, PLACENTA, Vol: 34, Pages: 1216-1222, ISSN: 0143-4004
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- Citations: 22
Qin Y, Liao P, You Y, et al., 2013, The Role of Fas Expression on the Occurrence of Immunosuppression in Severe Acute Pancreatitis, DIGESTIVE DISEASES AND SCIENCES, Vol: 58, Pages: 3300-3307, ISSN: 0163-2116
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- Citations: 12
Mitsuya K, Singh N, Sooranna SR, et al., 2013, Epigenetics of Human Myometrium: Relationship to the Length of Gestation and the Onset of Labor, 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 174A-174A, ISSN: 1933-7191
Singh N, Lei K, Sooranna SR, et al., 2013, Molecular Markers of Early and Established Labour in Human Myometrium., 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 300A-300A, ISSN: 1933-7191
Zollner J, Sooranna SR, Johnson MR, 2013, Myocardial Expression Level of Hypertrophy Marker Genes in a Guinea Pig Model of Left Ventricular Pressure Overload, 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 163A-163A, ISSN: 1933-7191
Lei K, Sooranna S, Bennett P, et al., 2013, Enhancement of Inflammation-Associated Genes by Progesterone in IL-1β-Stimulated Human Myometrial Cells, 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 161A-162A, ISSN: 1933-7191
Zheng X, Zhao H, Sooranna SR, et al., 2013, Development of a Clinically Relevant Model of Perinatal Hypoxic Ischaemic Encephalopathy in Mice., 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 300A-300A, ISSN: 1933-7191
Hua R, Pease JE, Cheng W, et al., 2013, Human Labour is Associated with a Decline in Myometrial Chemokine Receptor Expression: The Role of Prostaglandins, Oxytocin and Cytokines, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Vol: 69, Pages: 21-32, ISSN: 1046-7408
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- Citations: 5
Lei K, Chen L, Georgiou EX, et al., 2012, Progesterone acts via the nuclear glucocorticoid receptor to suppress IL-1 beta-Induced COX-2 expression in human term myometrial cells, PLoS ONE, Vol: 7, ISSN: 1932-6203
Progesterone is widely used to prolong gestation in women at risk of preterm labour (PTL), and acts at least in part via theinhibition of inflammatory cytokine-induced prostaglandin synthesis. This study investigates the mechanisms responsiblefor this inhibition in human myometrial cells. We used reporter constructs to demonstrate that interleukin 1beta (IL-1b)inhibits progesterone driven PRE activation via p65 activation and that IL-1b reduced progesterone driven gene expression(FKBP5). Conversely, we found that the activity of a p65-driven NFkB reporter construct was reduced by overexpression ofprogesterone receptor B (PRB) alone and that this was enhanced by the addition of MPA and that both MPA andprogesterone suppressed IL-1b-driven cyclo-oxygenase-2 (COX-2) expression. We found that over-expressed Halo-taggedPRB, but not PRA, bound to p65 and that in IL-1b-treated cells, with no overexpression of either PR or p65, activated p65bound to PR. However, we found that the ability of MPA to repress IL-1b-driven COX-2 expression was not enhanced byoverexpression of either PRB or PRA and that although the combined PR and GR antagonist Ru486 blocked the effects ofprogesterone and MPA, the specific PR antagonist, Org31710, did not, suggesting that progesterone and MPA act via GRand not PR. Knockdown using siRNA confirmed that both MPA and progesterone acted via GR and not PR or AR to repressIL-1b-driven COX-2 expression. We conclude that progesterone acts via GR to repress IL-1b-driven COX-2 activation and thatalthough the interaction between p65 and PRB may be involved in the repression of progesterone driven gene expression itdoes not seem to be responsible for progesterone repression of IL-1b-induced COX-2 expression.
Chatterjee R, Shah FT, Davis BA, et al., 2012, Prospective study of histomorphometry, biochemical bone markers and bone densitometric response to pamidronate in β-thalassaemia presenting with osteopenia-osteoporosis syndrome, 45th Annual Meeting and Exhibition of the American-Society-of-Hematology, Publisher: WILEY, Pages: 462-471, ISSN: 0007-1048
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- Citations: 17
Lee Y, Sooranna SR, Terzidou V, et al., 2012, Interactions between inflammatory signals and the progesterone receptor in regulating gene expression in pregnant human uterine myocytes, JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol: 16, Pages: 2487-2503, ISSN: 1582-1838
The absence of a fall in circulating progesterone levels has led to the concept that human labour is associated with ‘functional progesterone withdrawal’ caused through changes in the expression or function of progesterone receptor (PR). At the time of labour, the human uterus is heavily infiltrated with inflammatory cells, which release cytokines to create a ‘myometrial inflammation’ via NF‐κB activation. The negative interaction between NF‐κB and PR, may represent a mechanism to account for ‘functional progesterone withdrawal’ at term. Conversely, PR may act to inhibit NF‐κB function and so play a role in inhibition of myometrial inflammation during pregnancy. To model this inter‐relationship, we have used small interfering (si) RNA‐mediated knock‐down of PR in human pregnant myocytes and whole genome microarray analysis to identify genes regulated through PR. We then activated myometrial inflammation using IL‐1β stimulation to determine the role of PR in myometrial inflammation regulation. Through PR‐knock‐down, we found that PR regulates gene networks involved in myometrial quiescence and extracellular matrix integrity. Activation of myometrial inflammation was found to antagonize PR‐induced gene expression, of genes normally upregulated via PR. We found that PR does not play a role in repression of pro‐inflammatory gene networks induced by IL‐1β and that only MMP10 was significantly regulated in opposite directions by IL‐1β and PR. We conclude that progesterone acting through PR does not generally inhibit myometrial inflammation. Activation of myometrial inflammation does cause ‘functional progesterone withdrawal’ but only in the context of genes normally upregulated via PR.
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