Imperial College London
Alternate

DrDevSooranna

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 8846 7895s.sooranna Website

 
 
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Location

 

H.3.36Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{He:2018:10.3390/molecules23092189,
author = {He, Y and Li, Z and Wang, W and Sooranna, SR and Shi, Y and Chen, Y and Wu, C and Zeng, J and Tang, Q and Xie, H},
doi = {10.3390/molecules23092189},
journal = {Molecules},
title = {Chemical profiles and simultaneous quantification of Aurantii fructus by use of HPLC-Q-TOF-MS combined with GC-MS and HPLC methods},
url = {http://dx.doi.org/10.3390/molecules23092189},
volume = {23},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Aurantii fructus (AF) is a traditional Chinese medicine that has been used to improve gastrointestinal motility disorders for over a thousand years, but there is no exhaustive identification of the basic chemical components and comprehensive quality control of this herb. In this study, high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS) and gas chromatography coupled mass spectrometry (GC-MS) were employed to identify the basic chemical compounds, and high-performance liquid chromatography (HPLC) was developed to determine the major biochemical markers from AF extract. There were 104 compounds belonging to eight structure types, including 13 amino acids or peptides, seven alkaloids, 18 flavanones, 14 flavones, 15 polymethoxyflavonoids, six triterpenoids, nine coumarins, and 18 volatile oils, as well as four other compounds that were systematically identified as the basic components from AF, and among them, 41 compounds were reported for the first time. Twelve bioactive ingredients were chosen as the benchmark markers to evaluate the quality of AF. The analysis was completed with a gradient elution at a flow rate of 0.7 mL/min within 55 min. This efficient method was validated showing good linearity, precision, stability, repeatability and recovery. Furthermore, the method was successfully applied to the simultaneous determination of 12 chemical markers in different samples of AF. This study could be applied to the identification of multiple bioactive substances and improve the quality control of AF.
AU  - He,Y
AU  - Li,Z
AU  - Wang,W
AU  - Sooranna,SR
AU  - Shi,Y
AU  - Chen,Y
AU  - Wu,C
AU  - Zeng,J
AU  - Tang,Q
AU  - Xie,H
DO  - 10.3390/molecules23092189
PY  - 2018///
SN  - 1420-3049
TI  - Chemical profiles and simultaneous quantification of Aurantii fructus by use of HPLC-Q-TOF-MS combined with GC-MS and HPLC methods
T2  - Molecules
UR  - http://dx.doi.org/10.3390/molecules23092189
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30200226
UR  - http://hdl.handle.net/10044/1/63956
VL  - 23
ER  -
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