Imperial College London
Dr Samuel Marguerat

DrSamuelMarguerat

Faculty of Natural Sciences, Department of Mathematics

Visiting Researcher
 
 
 
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Contact

 

+44 (0)20 3313 8331samuel.marguerat Website

 
 
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Location

 

5003CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Robles-Rebollo:2022:10.1038/s41467-022-31192-9,
author = {Robles-Rebollo, I and Cuartero, S and Canellas-Socias, A and Wells, S and Karimi, MM and Mereu, E and Chivu, AG and Heyn, H and Whilding, C and Dormann, D and Marguerat, S and Rioja, I and Prinjha, RK and Stumpf, MPH and Fisher, AG and Merkenschlager, M},
doi = {10.1038/s41467-022-31192-9},
journal = {Nature Communications},
title = {Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters},
url = {http://dx.doi.org/10.1038/s41467-022-31192-9},
volume = {13},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Innate immune responses rely on inducible gene expression programmes which, in contrast to steady-state transcription, are highly dependent on cohesin. Here we address transcriptional parameters underlying this cohesin-dependence by single-molecule RNA-FISH and single-cell RNA-sequencing. We show that inducible innate immune genes are regulated predominantly by an increase in the probability of active transcription, and that probabilities of enhancer and promoter transcription are coordinated. Cohesin has no major impact on the fraction of transcribed inducible enhancers, or the number of mature mRNAs produced per transcribing cell. Cohesin is, however, required for coupling the probabilities of enhancer and promoter transcription. Enhancer-promoter coupling may not be explained by spatial proximity alone, and at the model locus Il12b can be disrupted by selective inhibition of the cohesinopathy-associated BET bromodomain BD2. Our data identify discrete steps in enhancer-mediated inducible gene expression that differ in cohesin-dependence, and suggest that cohesin and BD2 may act on shared pathways.
AU  - Robles-Rebollo,I
AU  - Cuartero,S
AU  - Canellas-Socias,A
AU  - Wells,S
AU  - Karimi,MM
AU  - Mereu,E
AU  - Chivu,AG
AU  - Heyn,H
AU  - Whilding,C
AU  - Dormann,D
AU  - Marguerat,S
AU  - Rioja,I
AU  - Prinjha,RK
AU  - Stumpf,MPH
AU  - Fisher,AG
AU  - Merkenschlager,M
DO  - 10.1038/s41467-022-31192-9
PY  - 2022///
SN  - 2041-1723
TI  - Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-022-31192-9
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35896525
UR  - http://hdl.handle.net/10044/1/98941
VL  - 13
ER  -
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