Imperial College London
Dr Samuel Marguerat

DrSamuelMarguerat

Faculty of Natural Sciences, Department of Mathematics

Visiting Researcher
 
 
 
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Contact

 

+44 (0)20 3313 8331samuel.marguerat Website

 
 
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Location

 

5003CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lemay:2016:10.1101/gad.280222.116,
author = {Lemay, JF and Marguerat, S and Larochelle, M and Liu, X and van, Nues R and Hunyadkürti, J and Hoque, M and Tian, B and Granneman, S and Bähler, J and Bachand, F},
doi = {10.1101/gad.280222.116},
journal = {Genes & Development},
pages = {1558--1572},
title = {The Nrd1-like protein Seb1 coordinates cotranscriptional 3′ end processing and polyadenylation site selection},
url = {http://dx.doi.org/10.1101/gad.280222.116},
volume = {30},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Termination of RNA polymerase II (RNAPII) transcription is associated with RNA 3′ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via a pathway that requires the Nrd1–Nab3–Sen1 (NNS) complex. Here we show that the Schizosaccharomyces pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3′ end processing factors, is enriched at the 3′ end of genes, and binds RNA motifs downstream from cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3′ untranslated region (UTR) length as a consequence of increased alternative polyadenylation. Given that Seb1 levels affected the recruitment of conserved 3′ end processing factors, our findings indicate that the conserved RNA-binding protein Seb1 cotranscriptionally controls alternative polyadenylation.
AU  - Lemay,JF
AU  - Marguerat,S
AU  - Larochelle,M
AU  - Liu,X
AU  - van,Nues R
AU  - Hunyadkürti,J
AU  - Hoque,M
AU  - Tian,B
AU  - Granneman,S
AU  - Bähler,J
AU  - Bachand,F
DO  - 10.1101/gad.280222.116
EP  - 1572
PY  - 2016///
SN  - 1549-5477
SP  - 1558
TI  - The Nrd1-like protein Seb1 coordinates cotranscriptional 3′ end processing and polyadenylation site selection
T2  - Genes & Development
UR  - http://dx.doi.org/10.1101/gad.280222.116
UR  - http://hdl.handle.net/10044/1/34291
VL  - 30
ER  -
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