Imperial College London
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Dr Sek-Shir Cheong

Faculty of Medicine, National Heart & Lung Institute

Research Associate
 
 
 
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Contact

 

sek-shir.cheong

 
 
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Location

 

Desk 10Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cheong:2020:10.3389/fcell.2020.577201,
author = {Cheong, SS and Akram, K and Metellan, C and Kim, S and Gaboriau, D and Hind, M and Del, Rio Hernandez A and Griffiths, M and Dean, C},
doi = {10.3389/fcell.2020.577201},
journal = {Frontiers in Cell and Developmental Biology},
title = {The planar polarity component Vangl2 is a key regulator of mechanosignaling},
url = {http://dx.doi.org/10.3389/fcell.2020.577201},
volume = {8},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - VANGL2 is a component of the planar cell polarity (PCP) pathway, which regulates tissue polarity and patterning. The Vangl2Lp mutation causes lung branching defects due to dysfunctional actomyosin-driven morphogenesis. Since the actomyosin network regulates cell mechanics, we speculated that mechanosignaling could be impaired when VANGL2 is disrupted. Here, we used live-imaging of precision-cut lung slices (PCLS) from Vangl2Lp/+ mice to determine that alveologenesis is attenuated as a result of impaired epithelial cell migration. Vangl2Lp/+ tracheal epithelial cells (TECs) and alveolar epithelial cells (AECs) exhibited highly disrupted actomyosin networks and focal adhesions (FAs). Functional assessment of cellular forces confirmed impaired traction force generation in Vangl2Lp/+ TECs. YAP signaling in Vangl2Lp airway epithelium was reduced, consistent with a role for VANGL2 in mechanotransduction. Furthermore, activation of RhoA signaling restored actomyosin organization in Vangl2Lp/+, confirming RhoA as an effector of VANGL2. This study identifies a pivotal role for VANGL2 in mechanosignaling, which underlies the key role of the PCP pathway in tissue morphogenesis.
AU  - Cheong,SS
AU  - Akram,K
AU  - Metellan,C
AU  - Kim,S
AU  - Gaboriau,D
AU  - Hind,M
AU  - Del,Rio Hernandez A
AU  - Griffiths,M
AU  - Dean,C
DO  - 10.3389/fcell.2020.577201
PY  - 2020///
SN  - 2296-634X
TI  - The planar polarity component Vangl2 is a key regulator of mechanosignaling
T2  - Frontiers in Cell and Developmental Biology
UR  - http://dx.doi.org/10.3389/fcell.2020.577201
UR  - http://hdl.handle.net/10044/1/84729
VL  - 8
ER  -
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