Imperial College London

Shahid A Khan

Faculty of MedicineFaculty of Medicine Centre

Professor of Practice (Haematology)
 
 
 
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Contact

 

+44 (0)20 3312 6254shahid.khan

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2016:10.1039/c6nr03376h,
author = {Liu, NJ and Chapman, R and Lin, Y and Bentham, A and Tyreman, M and Philips, N and Khan, SA and Stevens, MM},
doi = {10.1039/c6nr03376h},
journal = {Nanoscale},
pages = {11834--11839},
title = {Phospholipase A2 as a point of care alternative to serum amylase and pancreatic lipase},
url = {http://dx.doi.org/10.1039/c6nr03376h},
volume = {8},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Acute pancreatitis is a relatively common and potentially fatal condition, but the presenting symptoms are non-specific and diagnosis relies largely on the measurement of amylase activity by the hospital clinical laboratory. In this work we develop a point of care test for pancreatitis measuring concentration of secretory phospholipase A2 group IB (sPLA2-IB). Novel antibodies for sPLA2-IB were raised and used to design an ELISA and a lateral flow device (LFD) for the point of care measurement of sPLA2-IB concentration, which was compared to pancreatic amylase activity, lipase activity, and sPLA2-IB activity in 153 serum samples. 98 of these samples were obtained from the pathology unit of a major hospital and classified retrospectively according to presence or absence of pancreatitis, and the remaining 55 were obtained from commercial sources to serve as high lipase (n = 20), CA19-9 positive (n = 15), and healthy (n = 20) controls. sPLA2-IB concentration correlated well with the serum activity of both amylase and lipase, and performed at least as well as either markers in the differentiation of pancreatitis from controls.
AU - Liu,NJ
AU - Chapman,R
AU - Lin,Y
AU - Bentham,A
AU - Tyreman,M
AU - Philips,N
AU - Khan,SA
AU - Stevens,MM
DO - 10.1039/c6nr03376h
EP - 11839
PY - 2016///
SN - 2040-3372
SP - 11834
TI - Phospholipase A2 as a point of care alternative to serum amylase and pancreatic lipase
T2 - Nanoscale
UR - http://dx.doi.org/10.1039/c6nr03376h
UR - http://www.ncbi.nlm.nih.gov/pubmed/27228183
UR - http://hdl.handle.net/10044/1/33677
VL - 8
ER -