Imperial College London
Alternate

Shahid A Khan

Faculty of Medicine, Faculty of Medicine Centre

Professor of Practice (Hepatology)
 
 
 
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Contact

 

+44 (0)20 3312 6254shahid.khan

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2016:10.1039/c6nr03376h,
author = {Liu, NJ and Chapman, R and Lin, Y and Bentham, A and Tyreman, M and Philips, N and Khan, SA and Stevens, MM},
doi = {10.1039/c6nr03376h},
journal = {Nanoscale},
pages = {11834--11839},
title = {Phospholipase A2 as a point of care alternative to serum amylase and pancreatic lipase},
url = {http://dx.doi.org/10.1039/c6nr03376h},
volume = {8},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Acute pancreatitis is a relatively common and potentially fatal condition, but the presenting symptoms are non-specific and diagnosis relies largely on the measurement of amylase activity by the hospital clinical laboratory. In this work we develop a point of care test for pancreatitis measuring concentration of secretory phospholipase A2 group IB (sPLA2-IB). Novel antibodies for sPLA2-IB were raised and used to design an ELISA and a lateral flow device (LFD) for the point of care measurement of sPLA2-IB concentration, which was compared to pancreatic amylase activity, lipase activity, and sPLA2-IB activity in 153 serum samples. 98 of these samples were obtained from the pathology unit of a major hospital and classified retrospectively according to presence or absence of pancreatitis, and the remaining 55 were obtained from commercial sources to serve as high lipase (n = 20), CA19-9 positive (n = 15), and healthy (n = 20) controls. sPLA2-IB concentration correlated well with the serum activity of both amylase and lipase, and performed at least as well as either markers in the differentiation of pancreatitis from controls.
AU  - Liu,NJ
AU  - Chapman,R
AU  - Lin,Y
AU  - Bentham,A
AU  - Tyreman,M
AU  - Philips,N
AU  - Khan,SA
AU  - Stevens,MM
DO  - 10.1039/c6nr03376h
EP  - 11839
PY  - 2016///
SN  - 2040-3372
SP  - 11834
TI  - Phospholipase A2 as a point of care alternative to serum amylase and pancreatic lipase
T2  - Nanoscale
UR  - http://dx.doi.org/10.1039/c6nr03376h
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27228183
UR  - http://hdl.handle.net/10044/1/33677
VL  - 8
ER  -
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