Publications

BibTex format

@article{Dvinskikh:2023:10.1038/s41598-023-29419-w,
author = {Dvinskikh, L and Sparks, H and Brito, L and MacLeod, K and Harding, S and Dunsby, C},
doi = {10.1038/s41598-023-29419-w},
journal = {Scientific Reports},
pages = {1--14},
title = {Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture},
url = {http://dx.doi.org/10.1038/s41598-023-29419-w},
volume = {13},
year = {2023}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Improving cardiac function through stem-cell regenerative therapy requires functional and structural integration of the transplanted cells with the host tissue. Visualizing the electromechanical interaction between native and graft cells necessitates 3D imaging with high spatio-temporal resolution and low photo-toxicity. A custom light-sheet fluorescence microscope was used for volumetric imaging of calcium dynamics in co-cultures of adult rat left ventricle cardiomyocytes and human induced pluripotent stem cell-derived cardiomyocytes. Aberration-free remote refocus of the detection plane synchronously to the scanning of the light sheet along the detection axis enabled fast dual-channel 3D imaging at subcellular resolution without mechanical sample disturbance at up to 8 Hz over a ∼300 µm × 40 µm × 50 µm volume. The two cell types were found to undergo electrically stimulated and spontaneous synchronized calcium transients and contraction. Electromechanical coupling improved with co-culture duration, with 50% of adult-CM coupled after 24 h of co-culture, compared to 19% after 4 h (p = 0.0305). Immobilization with para-nitroblebbistatin did not prevent calcium transient synchronization, with 35% and 36% adult-CM coupled in control and treated samples respectively (p = 0.91), indicating that electrical coupling can be maintained independently of mechanotransduction.
AU  - Dvinskikh,L
AU  - Sparks,H
AU  - Brito,L
AU  - MacLeod,K
AU  - Harding,S
AU  - Dunsby,C
DO  - 10.1038/s41598-023-29419-w
EP  - 14
PY  - 2023///
SN  - 2045-2322
SP  - 1
TI  - Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-023-29419-w
UR  - https://www.nature.com/articles/s41598-023-29419-w
UR  - http://hdl.handle.net/10044/1/102905
VL  - 13
ER  -

Download

ProfessorSianHarding

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)