Imperial College London
Dr Silvia Santos

DrSilviaSantos

Faculty of Medicine, Institute of Clinical Sciences

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 8228silvia.santos Website

 
 
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Assistant

 

Miss Kayi Lydia Lawson +44 (0)20 3313 8265

 
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Location

 

2004CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Santos:2012:10.1016/j.cell.2012.05.028,
author = {Santos, SDM and Wollman, R and Meyer, T and Ferrell, JE},
doi = {10.1016/j.cell.2012.05.028},
journal = {Cell},
pages = {1500--1513},
title = {Spatial positive feedback at the onset of mitosis.},
url = {http://dx.doi.org/10.1016/j.cell.2012.05.028},
volume = {149},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mitosis is triggered by the activation of Cdk1-cyclin B1 and its translocation from the cytoplasm to the nucleus. Positive feedback loops regulate the activation of Cdk1-cyclin B1 and help make the process irreversible and all-or-none in character. Here we examine whether an analogous process, spatial positive feedback, regulates Cdk1-cyclin B1 redistribution. We used chemical biology approaches and live-cell microscopy to show that nuclear Cdk1-cyclin B1 promotes the translocation of Cdk1-cyclin B1 to the nucleus. Mechanistic studies suggest that cyclin B1 phosphorylation promotes nuclear translocation and, conversely, nuclear translocation promotes cyclin B1 phosphorylation, accounting for the feedback. Interfering with the abruptness of Cdk1-cyclin B1 translocation affects the timing and synchronicity of subsequent mitotic events, underscoring the functional importance of this feedback. We propose that spatial positive feedback ensures a rapid, complete, robust, and irreversible transition from interphase to mitosis and suggest that bistable spatiotemporal switches may be widespread in biological regulation.
AU  - Santos,SDM
AU  - Wollman,R
AU  - Meyer,T
AU  - Ferrell,JE
DO  - 10.1016/j.cell.2012.05.028
EP  - 1513
PY  - 2012///
SP  - 1500
TI  - Spatial positive feedback at the onset of mitosis.
T2  - Cell
UR  - http://dx.doi.org/10.1016/j.cell.2012.05.028
UR  - https://www.ncbi.nlm.nih.gov/pubmed/22726437
VL  - 149
ER  -
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