Imperial College London
Alternate

ProfessorStuartCook

Faculty of Medicine, Institute of Clinical Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 3313 1346stuart.cook

 
 
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Location

 

RF 16Sydney StreetRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Quaife:2023:10.1007/s12265-022-10288-z,
author = {Quaife, NM and Chothani, S and Schulz, JF and Lindberg, EL and Vanezis, K and Adami, E and O'Fee, K and Greiner, J and Litviuková, M and van, Heesch S and Whiffin, N and Hubner, N and Schafer, S and Rackham, O and Cook, SA and Barton, PJR},
doi = {10.1007/s12265-022-10288-z},
journal = {Journal of Cardiovascular Translational Research},
pages = {77--85},
title = {LINC01013 is a determinant of fibroblast activation and encodes a novel fibroblast-activating micropeptide},
url = {http://dx.doi.org/10.1007/s12265-022-10288-z},
volume = {16},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Myocardial fibrosis confers an almost threefold mortality risk in heart disease. There are no prognostic therapies and novel therapeutic targets are needed. Many thousands of unannotated small open reading frames (smORFs) have been identified across the genome with potential to produce micropeptides (< 100 amino acids). We sought to investigate the role of smORFs in myocardial fibroblast activation.Analysis of human cardiac atrial fibroblasts (HCFs) stimulated with profibrotic TGFβ1 using RNA sequencing (RNA-Seq) and ribosome profiling (Ribo-Seq) identified long intergenic non-coding RNA LINC01013 as TGFβ1 responsive and containing an actively translated smORF. Knockdown of LINC01013 using siRNA reduced expression of profibrotic markers at baseline and blunted their response to TGFβ1. In contrast, overexpression of a codon-optimised smORF invoked a profibrotic response comparable to that seen with TGFβ1 treatment, whilst FLAG-tagged peptide associated with the mitochondria.Together, these data support a novel LINC01013 smORF micropeptide-mediated mechanism of fibroblast activation.
AU  - Quaife,NM
AU  - Chothani,S
AU  - Schulz,JF
AU  - Lindberg,EL
AU  - Vanezis,K
AU  - Adami,E
AU  - O'Fee,K
AU  - Greiner,J
AU  - Litviuková,M
AU  - van,Heesch S
AU  - Whiffin,N
AU  - Hubner,N
AU  - Schafer,S
AU  - Rackham,O
AU  - Cook,SA
AU  - Barton,PJR
DO  - 10.1007/s12265-022-10288-z
EP  - 85
PY  - 2023///
SN  - 1937-5387
SP  - 77
TI  - LINC01013 is a determinant of fibroblast activation and encodes a novel fibroblast-activating micropeptide
T2  - Journal of Cardiovascular Translational Research
UR  - http://dx.doi.org/10.1007/s12265-022-10288-z
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35759180
UR  - http://hdl.handle.net/10044/1/98149
VL  - 16
ER  -
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