Imperial College London
Alternate

ProfessorStuartCook

Faculty of Medicine, Institute of Clinical Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 3313 1346stuart.cook

 
 
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Location

 

RF 16Sydney StreetRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chothani:2022:10.1016/j.molcel.2022.06.023,
author = {Chothani, SP and Adami, E and Widjaja, AA and Langley, SR and Viswanathan, S and Pua, CJ and Zhihao, NT and Harmston, N and D'Agostino, G and Whiffin, N and Mao, W and Ouyang, JF and Lim, WW and Lim, S and Lee, CQE and Grubman, A and Chen, J and Kovalik, JP and Tryggvason, K and Polo, JM and Ho, L and Cook, SA and Rackham, OJL and Schafer, S},
doi = {10.1016/j.molcel.2022.06.023},
journal = {Molecular Cell},
pages = {2885--2899},
title = {A high-resolution map of human RNA translation},
url = {http://dx.doi.org/10.1016/j.molcel.2022.06.023},
volume = {82},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Translated small open reading frames (smORFs) can have important regulatory roles and encode microproteins, yet their genome-wide identification has been challenging. We determined the ribosome locationsacross six primary human cell types and five tissues and detected 7,767 smORFs with translational profilesmatching those of known proteins. The human genome was found to contain highly cell-type- and tissuespecific smORFs and a subset that encodes highly conserved amino acid sequences. Changes in the translational efficiency of upstream-encoded smORFs (uORFs) and the corresponding main ORFs predominantlyoccur in the same direction. Integration with 456 mass-spectrometry datasets confirms the presence of 603small peptides at the protein level in humans and provides insights into the subcellular localization of thesesmall proteins. This study provides a comprehensive atlas of high-confidence translated smORFs derivedfrom primary human cells and tissues in order to provide a more complete understanding of the translatedhuman genome.
AU  - Chothani,SP
AU  - Adami,E
AU  - Widjaja,AA
AU  - Langley,SR
AU  - Viswanathan,S
AU  - Pua,CJ
AU  - Zhihao,NT
AU  - Harmston,N
AU  - D'Agostino,G
AU  - Whiffin,N
AU  - Mao,W
AU  - Ouyang,JF
AU  - Lim,WW
AU  - Lim,S
AU  - Lee,CQE
AU  - Grubman,A
AU  - Chen,J
AU  - Kovalik,JP
AU  - Tryggvason,K
AU  - Polo,JM
AU  - Ho,L
AU  - Cook,SA
AU  - Rackham,OJL
AU  - Schafer,S
DO  - 10.1016/j.molcel.2022.06.023
EP  - 2899
PY  - 2022///
SN  - 1097-2765
SP  - 2885
TI  - A high-resolution map of human RNA translation
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2022.06.023
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000865740700004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.sciencedirect.com/science/article/pii/S1097276522006062
VL  - 82
ER  -
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