Publications
80 results found
SMITH SF, 1990, A POTENTIAL MECHANISM FOR SOME ANTIINFLAMMATORY EFFECTS OF THE GLUCOCORTICOIDS, RESPIRATORY MEDICINE, Vol: 84, Pages: 435-436, ISSN: 0954-6111
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- Citations: 3
SMITH SF, GOULDING NJ, GODOLPHIN JL, et al., 1990, AN ASSAY FOR THE ASSESSMENT OF LIPOCORTIN-1 LEVELS IN HUMAN LUNG LAVAGE FLUID, JOURNAL OF IMMUNOLOGICAL METHODS, Vol: 131, Pages: 119-125, ISSN: 0022-1759
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- Citations: 21
Smith SF, Tetley TD, Guz A, et al., 1990, Detection of lipocortin 1 in human lung lavage fluid: lipocortin degradation as a possible proteolytic mechanism in the control of inflammatory mediators and inflammation, Environmental Health Perspectives, Vol: 85, Pages: 135-144, ISSN: 1552-9924
TETLEY TD, SMITH SF, WINNING AJ, et al., 1989, THE ACUTE EFFECT OF CIGARETTE-SMOKING ON THE NEUTROPHIL ELASTASE INHIBITORY CAPACITY OF PERIPHERAL LUNG LAVAGE FROM ASYMPTOMATIC VOLUNTEERS, EUROPEAN RESPIRATORY JOURNAL, Vol: 2, Pages: 802-810, ISSN: 0903-1936
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- Citations: 10
Smith SF, 1989, A simple method for western blotting very thin SDS-polyacrylamide gels., Biotechniques, Vol: 7, Pages: 1060-1064, ISSN: 0736-6205
SMITH SF, GUZ A, WINNING AJ, et al., 1988, COMPARISON OF HUMAN-LUNG SURFACE PROTEIN PROFILES FROM THE CENTRAL AND PERIPHERAL AIRWAYS SAMPLED USING 2 REGIONAL LAVAGE TECHNIQUES, EUROPEAN RESPIRATORY JOURNAL, Vol: 1, Pages: 792-800, ISSN: 0903-1936
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- Citations: 17
Smith SF, Guz A, Burton GH, et al., 1988, The elastase: anti-elastase profile in lung lavage from sarcoidosis patients., Sarcoidosis, Vol: 5, Pages: 31-37, ISSN: 0393-1447
One feature of pulmonary sarcoidosis is an increase of lymphocyte numbers in bronchoalveolar lavage fluid (BALF). In some patients the number of polymorphonuclear neutrophils (PMN) also rises. It has been suggested that the prognosis for the latter subjects is likely to be worse than that for the former, but the damaging agent (or agents) released by the PMN have not yet been identified. Therefore, in the current study, the activity of one neutrophilic enzyme, elastase, which is known to damage the pulmonary interstitium, has been assessed in sarcoid BALF and compared to BALF from subjects matched for age, sex and smoking status. As BALF also contains locally-produced and serum-derived inhibitors of elastase, levels of which may change in subjects with sarcoidosis, the serine protease inhibitory capacity and the levels of three anti-elastases have been measured in the samples. Levels of the serum-derived antiproteases, alpha 1 proteinase inhibitor (alpha 1PI) and alpha 2 macroglobulin (alpha 2M) were found to be significantly increased. However, alpha 1PI/albumin and alpha 2M/albumin ratios were unchanged, suggesting that the increased levels were due to an increased permeability of the alveolar-capillary barrier. The total protease inhibitory capacity was elevated and this could be entirely explained by the raised levels of alpha 1PI and alpha 2M. Levels of the locally-produced inhibitor were unaltered. The elastolytic capacity of sarcoid BALF was unchanged. Thus, the elastase: anti-elastase balance was shifted against elastolytic activity.(ABSTRACT TRUNCATED AT 250 WORDS)
SMITH SF, GUZ A, BURTON GH, et al., 1988, COMPARISON OF ELASTOLYTIC ACTIVITY IN LUNG LAVAGE FROM CURRENT, EX-SMOKERS AND NON-SMOKERS, LIFE SCIENCES, Vol: 43, Pages: 459-464, ISSN: 0024-3205
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- Citations: 6
SMITH SF, GUZ A, TETLEY TD, 1987, PROFILE OF ELASTASE ACTIVITY RELEASED BY HUMAN-LUNG LAVAGE CELLS IN CULTURE, CLINICAL SCIENCE, Vol: 72, Pages: P84-P84, ISSN: 0143-5221
Tetley TD, Smith SF, Burton GH, et al., 1987, Effects of cigarette smoking and drugs on respiratory tract proteases and antiproteases., Eur J Respir Dis Suppl, Vol: 153, Pages: 93-102, ISSN: 0106-4347
Increased pulmonary proteolytic (elastolytic) activity is thought to be the primary cause of emphysema and may also play a rôle in the pathology of bronchitis. These diseases are common amongst tobacco smokers. Serum-derived alpha 1-proteinase inhibitor (alpha 1PI) and locally produced protease inhibitors normally protect the pulmonary epithelium from proteolytic attack, but tobacco smoke can inactivate these antiproteases by oxidative and non-oxidative mechanisms. Bronchoalveolar lavage fluid (BALF) samples lung surface components and most studies show that there is elevated elastolytic activity in smokers' BALF. Whether antiproteolytic capacity is reduced in these samples remains debatable. A selective lavage technique is described which independently samples central and peripheral epithelium from the same subject. Analysis demonstrates a protease-antiprotease imbalance which can differ in central and peripheral lavage and which could be significant in the development of obstructive airways disease. Therapeutic approaches include augmenting antiprotease potential using genetically engineered, oxidant-resistant alpha 1PI or synthetic peptide inhibitors.
SMITH SF, GUZ A, BURTON GH, et al., 1986, ACID-STABLE LOW-MOLECULAR MASS PROTEINASE-INHIBITORS IN HUMAN-LUNG LAVAGE, BIOLOGICAL CHEMISTRY HOPPE-SEYLER, Vol: 367, Pages: 183-189, ISSN: 0177-3593
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- Citations: 11
Smith SF, Guz A, Cooke NT, et al., 1986, Effects of cigarette smoke on antiproteases at the human lung surface., Eur J Respir Dis Suppl, Vol: 146, Pages: 139-143, ISSN: 0106-4347
SMITH SF, GUZ A, COOKE NT, et al., 1986, EFFECTS OF CIGARETTE-SMOKE ON ANTIPROTEASES AT THE HUMAN-LUNG SURFACE, EUROPEAN JOURNAL OF RESPIRATORY DISEASES, Vol: 69, Pages: 139-143, ISSN: 0106-4339
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- Citations: 1
SMITH SF, GUZ A, COOKE NT, et al., 1986, ACUTE EFFECTS OF CIGARETTE-SMOKE ON ANTIPROTEASES AT THE HUMAN-LUNG SURFACE, EUROPEAN JOURNAL OF RESPIRATORY DISEASES, Vol: 69, Pages: A158-A158, ISSN: 0106-4339
SMITH SF, GUZ A, COOKE NT, et al., 1985, COMPARISON OF EXTRACELLULAR ACTIVITY IN LUNG LAVAGE FROM CURRENT, EX-SMOKER AND NON-SMOKER, CLINICAL SCIENCE, Vol: 69, Pages: P34-P34, ISSN: 0143-5221
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- Citations: 1
TETLEY TD, SMITH SF, BURTON GH, et al., 1985, ACUTE EFFECTS OF CIGARETTE-SMOKING ON THE ANTIPROTEASE - PROTEASE BALANCE OF THE HUMAN-LUNG SURFACE, CLINICAL SCIENCE, Vol: 69, Pages: P58-P58, ISSN: 0143-5221
SMITH SF, GUZ A, COOKE NT, et al., 1985, EXTRACELLULAR ELASTOLYTIC ACTIVITY IN HUMAN-LUNG LAVAGE - A COMPARATIVE-STUDY BETWEEN SMOKERS AND NON-SMOKERS, CLINICAL SCIENCE, Vol: 69, Pages: 17-27, ISSN: 0143-5221
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- Citations: 34
STOCKLEY RA, MORRISON HM, SMITH S, et al., 1984, LOW-MOLECULAR MASS BRONCHIAL PROTEINASE-INHIBITOR AND ALPHA-1-PROTEINASE INHIBITOR IN SPUTUM AND BRONCHOALVEOLAR LAVAGE, HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, Vol: 365, Pages: 587-595, ISSN: 0018-4888
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- Citations: 42
SMITH SF, GUZ A, TETLEY TD, 1984, THE EFFECT OF INTERACTIONS WITH LEUKOCYTE ELASTASE ON THE QUANTITATION OF ALPHA-1-PROTEINASE INHIBITOR BY ROCKET IMMUNOELECTROPHORESIS, CLINICAL SCIENCE, Vol: 66, Pages: P24-P25, ISSN: 0143-5221
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- Citations: 1
SMITH SF, GUZ A, TETLEY TD, 1984, CHARACTERIZATION OF ELASTASE ACTIVITY IN BRONCHOALVEOLAR LAVAGE FROM SMOKERS AND NON-SMOKERS, CLINICAL SCIENCE, Vol: 66, Pages: P6-P7, ISSN: 0143-5221
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