Imperial College London
Alternate

Dr Sue Wilson

Faculty of Medicine, Department of Brain Sciences

Honorary Senior Research Fellow
 
 
 
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Contact

 

sue.wilson

 
 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nahar:2016:jat/bkv125,
author = {Nahar, LK and Cordero, R and Nutt, D and Lingford-Hughes, A and Turton, S and Durant, C and Wilson, S and Paterson, S},
doi = {jat/bkv125},
journal = {Journal of Analytical Toxicology},
pages = {117--123},
title = {Validated method for the quantification of baclofen in human plasma using solid-phase extraction and liquid chromatography–tandem mass spectrometry},
url = {http://dx.doi.org/10.1093/jat/bkv125},
volume = {40},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A highly sensitive and fully validated method was developed for the quantification of baclofen in human plasma. After adjusting the pH of the plasma samples using a phosphate buffer solution (pH 4), baclofen was purified using mixed mode (C8/cation exchange) solid-phase extraction (SPE) cartridges. Endogenous water-soluble compounds and lipids were removed from the cartridges before the samples were eluted and concentrated. The samples were analyzed using triple-quadrupoleliquid chromatography–tandem mass spectrometry (LC–MS-MS) with triggered dynamic multiple reaction monitoring mode for simultaneous quantification and confirmation. The assay was linear from 25 to 1,000 ng/mL (r2 > 0.999; n = 6). Intraday (n = 6) and interday (n = 15) imprecisions (% relative standard deviation) were <5%, and the average recovery was 30%. The limit of detection of the method was 5 ng/mL, and the limit of quantification was 25 ng/mL. Plasma samples from healthymale volunteers (n = 9, median age: 22) given two single oral doses of baclofen (10 and 60 mg) on nonconsecutive days were analyzed to demonstrate method applicability.
AU  - Nahar,LK
AU  - Cordero,R
AU  - Nutt,D
AU  - Lingford-Hughes,A
AU  - Turton,S
AU  - Durant,C
AU  - Wilson,S
AU  - Paterson,S
DO  - jat/bkv125
EP  - 123
PY  - 2016///
SN  - 0146-4760
SP  - 117
TI  - Validated method for the quantification of baclofen in human plasma using solid-phase extraction and liquid chromatography–tandem mass spectrometry
T2  - Journal of Analytical Toxicology
UR  - http://dx.doi.org/10.1093/jat/bkv125
UR  - http://hdl.handle.net/10044/1/58494
VL  - 40
ER  -
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