Imperial College London
Alternate

ProfessorThomasBrand

Faculty of Medicine, National Heart & Lung Institute

Chair in Developmental Dynamics
 
 
 
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Contact

 

+44 (0)20 7594 8744t.brand Website CV

 
 
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Assistant

 

Miss Cheryl Costello +44 (0)20 7594 3001

 
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Location

 

433ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tibbo:2022:10.1016/j.yjmcc.2022.01.001,
author = {Tibbo, AJ and Mika, D and Dobi, S and Ling, J and McFall, A and Tejeda, GS and Blair, C and MacLeod, R and MacQuaide, N and Gök, C and Fuller, W and Smith, BO and Smith, GL and Vandecasteele, G and Brand, T and Baillie, GS},
doi = {10.1016/j.yjmcc.2022.01.001},
journal = {Journal of Molecular and Cellular Cardiology},
pages = {86--102},
title = {Phosphodiesterase type 4 anchoring regulates cAMP signaling to Popeye domain-containing proteins.},
url = {http://dx.doi.org/10.1016/j.yjmcc.2022.01.001},
volume = {165},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cyclic AMP is a ubiquitous second messenger used to transduce intracellular signals from a variety of Gs-coupled receptors. Compartmentalisation of protein intermediates within the cAMP signaling pathway underpins receptor-specific responses. The cAMP effector proteins protein-kinase A and EPAC are found in complexes that also contain phosphodiesterases whose presence ensures a coordinated cellular response to receptor activation events. Popeye domain containing (POPDC) proteins are the most recent class of cAMP effectors to be identified and have crucial roles in cardiac pacemaking and conduction. We report the first observation that POPDC proteins exist in complexes with members of the PDE4 family in cardiac myocytes. We show that POPDC1 preferentially binds the PDE4A sub-family via a specificity motif in the PDE4 UCR1 region and that PDE4s bind to the Popeye domain of POPDC1 in a region known to be susceptible to a mutation that causes human disease. Using a cell-permeable disruptor peptide that displaces the POPDC1-PDE4 complex we show that PDE4 activity localized to POPDC1 modulates cycle length of spontaneous Ca2+ transients firing in intact mouse sinoatrial nodes.
AU  - Tibbo,AJ
AU  - Mika,D
AU  - Dobi,S
AU  - Ling,J
AU  - McFall,A
AU  - Tejeda,GS
AU  - Blair,C
AU  - MacLeod,R
AU  - MacQuaide,N
AU  - Gök,C
AU  - Fuller,W
AU  - Smith,BO
AU  - Smith,GL
AU  - Vandecasteele,G
AU  - Brand,T
AU  - Baillie,GS
DO  - 10.1016/j.yjmcc.2022.01.001
EP  - 102
PY  - 2022///
SN  - 0022-2828
SP  - 86
TI  - Phosphodiesterase type 4 anchoring regulates cAMP signaling to Popeye domain-containing proteins.
T2  - Journal of Molecular and Cellular Cardiology
UR  - http://dx.doi.org/10.1016/j.yjmcc.2022.01.001
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34999055
UR  - https://www.sciencedirect.com/science/article/pii/S0022282822000025?via%3Dihub
UR  - http://hdl.handle.net/10044/1/93761
VL  - 165
ER  -
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