Publications
286 results found
Radomska A, Singhal S, Ye H, et al., 2008, Biocompatible ion selective electrode for monitoring metabolic activity during the growth and cultivation of human cells, Biosensors and Bioelectronics, Vol: 24, Pages: 435-441, ISSN: 1873-4235
Cass AEG, 2007, Organometallic compounds in biosensing, Comprehensive Organometallic Chemistry III, Pages: 589-602, ISBN: 9780080450476
Toumazou C, Cass T, 2007, Cell-bionics: tools for real-time sensor processing, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 362, Pages: 1321-1328, ISSN: 0962-8436
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- Citations: 1
Cass AEG, 2007, Building Biolelectronic Interfaces, Electronics Letters, Vol: 43, Pages: 903-905
Lim M, Ye H, Panoskaltsis N, et al., 2007, Intelligent Bioprocessing for Haemotopoietic Cell Cultures Using Monitoring and Design of Experiments, Biotechnology Advances, Vol: 25, Pages: 353-368, ISSN: 0734-9750
Cass A, Cunningham J, Anderson K, et al., 2007, Decision-making about suitability for kidney transplantation: Results of a national survey of Australian nephrologists., Nephrology (Carlton), Vol: 12, Pages: 299-304, ISSN: 1320-5358
AIM: This study aimed to elucidate the factors affecting nephrologists' decision-making on patients' suitability for kidney transplantation. Given the reduced access to transplantation for Indigenous Australians, the role of patient's ethnicity was of particular interest. METHODS: A postal survey of practising nephrologists and trainees was undertaken in Australia. Each participant was provided with a unique set of 15 hypothetical patient descriptions, with demographic, clinical and behavioural factors randomly generated to ensure an overall balance of factors across the cases. The main outcome measure was whether kidney transplantation was recommended. RESULTS: Responding nephrologists and trainees were more likely to recommend transplantation for hypothetical patients who were young, of normal weight and described as compliant. They were less likely to recommend transplantation for smokers, or for people with diabetes or heart disease. No significant differences related to the patients' sex or ethnicity. The geographical location of the respondent was a significant determinant, with differences according to their State/Territory and their metropolitan/non-metropolitan location. CONCLUSION: When all other factors were held constant, nephrologists and trainees appear to base their decision-making regarding suitability for transplant on clinical and behavioural factors, rather than on the basis of ethnicity or sex. In practice, however, clinical and behavioural factors cluster with ethnicity, and this is likely to contribute to the current poor access to transplantation for Indigenous end-stage kidney disease patients. Apparent differences in decision-making according to the respondent's location may reflect variations in practice across the country.
Huang X, Zhang X-E, Zhou Y-F, et al., 2007, Construction of a high sensitive <i>Escherichia coli</i> alkaline phosphatase reporter system for screening affinity peptides, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, Vol: 70, Pages: 435-439, ISSN: 0165-022X
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- Citations: 4
Edel JB, Lahoud P, Cass AEG, et al., 2007, Discrimination between single <i>Escherichia coli</i> cells using time-resolved confocal spectroscopy, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 111, Pages: 1129-1134, ISSN: 1520-6106
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- Citations: 4
Hassard J, Cass T, 2007, High-throughput monitoring of protein folding, Innovations in Pharmaceutical Technology, ISSN: 1471-7204
A novel technology has been developed for the rapid and accurate analysis of the folded state of proteins, enabling protein folding to be monitored on a high-throughput basis for drug discovery and manufacturing processes.
Yue X, Drakakis EM, Hua Y, et al., 2007, An On-line, Multi-Parametric, Multi-Channel Physicochemical Monitoring Platform for Stem Cell Culture Bioprocessing, IEEE International Symposium on Circuits and Systems (ISCAS), Publisher: IEEE, Pages: 1215-1218
Yue X, Drakakis EA, Ye H, et al., 2007, An on-line, multi-parametric, multi-channel physicochemical monitoring platform for stem cell culture bioprocessing, IEEE International Symposium on Circuits and Systems, Publisher: IEEE, Pages: 1215-+, ISSN: 0271-4302
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- Citations: 1
Huang X, Zhang X-E, Zhou Y-F, et al., 2006, Directed evolution of the 5'-untranslated region of the phoA gene in Escherichia coli simultaneously yields a stronger promoter and a stronger Shine-Dalgarno sequence., Biotechnol J, Vol: 1, Pages: 1275-1282
Directed evolution has been widely applied for gene improvement through random mutagenesis of coding sequences. Through error-prone PCR both in the coding sequence and the regulatory sequence of E. coli alkaline phosphatase, the cellular enzyme activity has been efficiently enhanced. Sequence analysis revealed that the resultant mutant 34-B12, which showed a sevenfold increased enzyme activity at the cellular level, contains three mutations in the regulatory sequence and another three mutations in the coding sequence. Activity assays of the enzyme containing the corresponding amino acid substitutions proved that the amino acid mutations contribute only to a small portion to the increased cellular enzyme activity. So the mutations in the 5'-untranslated region were analyzed separately and combinationally. The results suggested that one mutation yielded a stronger promoter and the other two mutations both elevated the E. coli alkaline phosphatase expression at the translational level; moreover, a stronger Shine-Dalgarno sequence was generated.
Liu FM, Kollensperger PA, Green M, et al., 2006, A note on distance dependence in surface enhanced Raman spectroscopy, CHEMICAL PHYSICS LETTERS, Vol: 430, Pages: 173-176, ISSN: 0009-2614
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- Citations: 19
Topoglidis E, Campbell CJ, Cass AEG, et al., 2006, Nitric oxide biosensors based on the immobilization of hemoglobin on mesoporous titania electrodes, ELECTROANALYSIS, Vol: 18, Pages: 882-887, ISSN: 1040-0397
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- Citations: 46
Bedford C, Cas T, Francois I, et al., 2006, Glycomics: From glycobiology to diagnostics and therapeutics., Pages: 163-172, ISSN: 0214-0934
The Royal Society of Chemistry Biotechnology Group and Chemical Biology Forum held a two-day symposium on December 12-13, 2005, in London. The meeting was designed to give an overview of the exciting new technologies being applied to study complex carbohydrates from their sequence analysis, characterization and function through to the development of novel pharmacological approaches to diagnose and alleviate polysaccharide-mediated diseases. The meeting, which also included a poster session, highlighted the multidisciplinary nature of the research and development and the exciting advances being made in this field.
Taylor PM, Cass AEG, Yacoub MH, 2006, Extracellular Matrix Scaffolds for Tissue Engineering Heart Valves., Progress in Pediatric Cardiology, Vol: 21, Pages: 219-225, ISSN: 1058-9813
Green M, Liu F-M, Cohen L, et al., 2006, SERS platforms for high density DNA arrays, Faraday Discuss., Vol: 132, Pages: 269-280-269-280
Surface Enhanced Raman Scattering (SERS) gives rise to analytical applications with much promise. In our approach three steps are necessary. We require a SERS platform of high enhancement. This has been achieved using the special technique of Island Lithography, combined with Ag deposition by galvanic exchange, yielding an enhancement factor of 10. Probe oligonucleotide molecules are attached to a specific area on the platform, at the optimized surface concentration, using thiolated single stranded (ss) DNA molecules. The optimum surface concentration has been determined and interpreted in the light of the polyelectrolyte behaviour of ssDNA. Finally the change in SERS produced by hybridisation of the probe molecules to a target DNA molecule is measured. Highly discernible changes have been obtained. No change in probe signal is seen when presented with one base mismatched target. From this work it is concluded that the prospects for label-free DNA detection in high-density arrays is now close to achievement.
Yue X, Drakakis EM, Toumazou C, et al., 2006, 8x16 Channel Physiological Monitoring Platform of Stem Cell Culture Systems, IEEE Biomedical Circuits and Systems Conference (BioCAS), Publisher: IEEE, Pages: 106-109
Zhang JK, Cass AEG, 2005, Kinetic study of site directed and randomly immobilized his-tag alkaline phosphatase by flow injection chemiluminescence, JOURNAL OF MOLECULAR RECOGNITION, Vol: 19, Pages: 243-246, ISSN: 0952-3499
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- Citations: 6
Cass T, 2005, Protein engineering for biosensor design, Pages: 195-215, ISSN: 0537-9989
The use of biological materials (molecules, cells or tissues) to impact specificity and sensitivity to sensors is well established and has had some notable successes, for example in blood glucose measurement. However the use of 'natural' biological materials suffers from the singular disadvantage that their properties have evolved to fit their biological function rather their application to sensor technology. One approach that overcomes this disadvantage is the use of biologically inspired synthetic materials. The alternative, which I will illustrate in this presentation, is to re-engineer the biology to overcome the inherent limitations of natural materials. © Imperial College.
Eady NAJ, Jesmin NAJ, Servos S, et al., 2005, Probing the function of <i>Mycobacterium tuberculosis</i> catalase-peroxidase by site-directed mutagenesis, DALTON TRANSACTIONS, Pages: 3495-3500, ISSN: 1477-9226
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- Citations: 4
Cooper JM, Cass AEG, 2005, Lab-on-a-chip and microarrays, Chemistry & Industry, Vol: 20, Pages: 653-655, ISSN: 0009-3068
Shi JX, Zhang XE, Xie WH, et al., 2004, Improvement of homogeneity of analytical biodevices by gene manipulation, ANALYTICAL CHEMISTRY, Vol: 76, Pages: 632-638, ISSN: 0003-2700
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- Citations: 18
Cooper JM, Cass AEG, 2004, Biosensors: a practical approach, Publisher: Oxford University Press, ISBN: 9780199638451
Wada A, Mie M, Aizawa M, et al., 2003, Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 125, Pages: 16228-16234, ISSN: 0002-7863
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- Citations: 41
Noble JE, Ganju P, Cass AEG, 2003, Fluorescent peptide probes for high-throughput measurement of protein phosphatases, ANALYTICAL CHEMISTRY, Vol: 75, Pages: 2042-2047, ISSN: 0003-2700
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- Citations: 24
Cass A, Cunningham J, Snelling P, et al., 2003, Renal transplantation for Indigenous Australians: identifying the barriers to equitable access., Ethn Health, Vol: 8, Pages: 111-119, ISSN: 1355-7858
OBJECTIVE: To assess Indigenous Australians' access to renal transplantation, compared with non-Indigenous Australians. To examine whether disparities are due to a lower rate of acceptance onto the waiting list and/or a lower rate of moving from the list to transplantation. DESIGN: National cohort study using data from the Australian and New Zealand Dialysis and Transplant Registry. We included all end-stage renal disease (ESRD) patients under 65 years of age who started treatment in Australia between January 1993 and December 1998. We used survival analysis to examine the time from commencement of renal replacement therapy (RRT) to transplantation. We measured time from commencement of RRT to acceptance onto the waiting list (stage 1), and time from acceptance onto the waiting list to transplantation (stage 2). The main outcome measures were (1) acceptance onto the waiting list and (2) receipt of a transplant, before 31 March 2000. RESULTS: Indigenous patients had a lower transplantation rate (adjusted Indigenous: non-Indigenous rate ratio 0.32, 95% CI 0.25-0.40). They had both a lower rate of acceptance onto the waiting list (adjusted rate ratio 0.50, 95% CI 0.44-0.57) and a lower rate of moving from the list to transplantation (adjusted rate ratio 0.50, 95% CI 0.38-0.65). The disparities were not explained by differences in age, sex, co-morbidities or cause of renal disease. CONCLUSIONS: Indigenous Australians face barriers to acceptance onto the waiting list and to moving from the list to transplantation. Further research to identify the causes could facilitate strategies to improve equity in transplantation.
Xu HF, Zhang XE, Zhang ZP, et al., 2003, The in vitro directed evolution of E. coli alkaline phosphatase, Progress in Biochemistry and Biophysics, Vol: 30, Pages: 89-94, ISSN: 1000-3282
The evolution of phoA gene fragment distant from the Asp101-Ser102-Ala103 encoding region to increase the catalytic activity of EAP with a single mutant D101S as parent was directed. Through two cycles of error prone PCR, coupled with a sensitive screening method, an evolved variant 4-186 was obtained. Its catalytic activity was 3-fold higher than that of D101S parent and 35-fold more active than wild-type EAP. The kinetic analysis indicated that the evolved enzyme exhibits a higher substrate binding ability and a higher catalytic efficiency than the D101S parent enzyme. DNA sequence revealed that 4-186 contains two amino acid substitutions, K167R and S374C, both of which locate neither the substrate-binding sites nor the metal-binding sites of EAP.
Xu HF, Zhang XE, Zhang ZP, et al., 2003, Directed evolution of <i>E-coli</i> alkaline phosphatase towards higher catalytic activity, BIOCATALYSIS AND BIOTRANSFORMATION, Vol: 21, Pages: 41-47, ISSN: 1024-2422
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- Citations: 11
Simon E, Halliwell CM, Toh CS, et al., 2002, Oxidation of NADH produced by a lactate dehydrogenase immobilised on poly(aniline)-poly(anion) composite films, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, Vol: 538, Pages: 253-259, ISSN: 0022-0728
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- Citations: 22
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