Professor Tony Cass

Halliwell CM, Simon E, Toh CS, Bartlett PN, Cass AEGet al., 2002, A method for the determination of enzyme mass loading on an electrode surface through radioisotope labelling, BIOSENSORS & BIOELECTRONICS, Vol: 17, Pages: 965-972, ISSN: 0956-5663

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• Citations: 6

Chen LQ, Zhang XE, Xie WH, Zhou YF, Zhang ZP, Cass AEGet al., 2002, Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor, BIOSENSORS & BIOELECTRONICS, Vol: 17, Pages: 851-857, ISSN: 0956-5663

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• Citations: 42

Bi L-J, Zhou Y-F, Deng J-Y, Zhang X-E, Zhang C-G, Cass AEGet al., 2002, [High expression and identification of DNA mismatch repair gene mutS in Escherichia coli]., Sheng Wu Gong Cheng Xue Bao, Vol: 18, Pages: 536-540, ISSN: 1000-3061

DNA mismatch repair gene mutS (2.56 kb) was PCR modified and cloned into a secretive prokaryotic expression vector pET32a (+) which carries a N-terminal His.tag + and thioredoxin sequence. MutS protein was expressed with high level after IPTG induction using the strain E. coli AD494(DE3). SDS-PAGE revealed that the expected protein with a molecular weight of 108 kD which is about 35% of the total bacterial proteins is almost soluble. The expected protein was purified directly by immobilized metal (Ni2+) chelation affinity chromatography and the purity is over 90%. MutS protein activity verified using mismatch DNA showed that the expression product can recognize and bind to base-pair mismatch specifically.

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Cooper JM, Cass AEG, 2002, Miniaturized screening technologies for drug discovery, y593kzg on High-Throughput Screening - The Way Ahead, Publisher: PORTLAND PRESS, Pages: 802-806, ISSN: 0300-5127

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• Citations: 3

Xu HF, Zhang XE, Zhang YM, Cass AEGet al., 2002, Recent advances in <i>in vitro</i> molecular directed evolution, PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, Vol: 29, Pages: 518-522, ISSN: 1000-3282

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• Citations: 4

Zhou Y-F, Zhang X-E, Cass AEG, 2002, [Current researches on molecular enzyme engineering]., Sheng Wu Gong Cheng Xue Bao, Vol: 18, Pages: 401-406, ISSN: 1000-3061

The developments of recombinant DNA technology and structural biology make it possible to modify enzyme in molecular level. Scientists show growing interests in the evolution or functional fusion of enzymes. Recent advances and applications of the molecular enzyme engineering are reviewed and discussed in this article.

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Halliwell CM, Simon E, Toh CS, Bartlett PN, Cass AEGet al., 2002, Immobilisation of lactate dehydrogenase on poly(aniline)-poly(acrylate) and poly(aniline)-poly-(vinyl sulphonate) films for use in a lactate biosensor, ANALYTICA CHIMICA ACTA, Vol: 453, Pages: 191-200, ISSN: 0003-2670

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• Citations: 51

Cass T, Dunwell J, Wackett LP, Gilardi G, Kost TA, Condreay P, Projan S, Hugenholtz J, Kleerebezem M, Turner NJ, Speight REet al., 2002, Biotechnology. Paper Alert., Curr Opin Biotechnol, Vol: 13, Pages: 1-8, ISSN: 0958-1669

A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in biotechnology.

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Halliwell CM, Simon E, Toh CS, Cass AEG, Bartlett PNet al., 2002, The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes, BIOELECTROCHEMISTRY, Vol: 55, Pages: 21-23, ISSN: 1567-5394

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• Citations: 29

Simon E, Halliwell CM, Toh CS, Cass AEG, Bartlett PNet al., 2002, Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications, 26th International Symposium on Bioelectrochemistry and Bioenergetics, Publisher: ELSEVIER SCIENCE SA, Pages: 13-15, ISSN: 1567-5394

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• Citations: 50

Tsotsou GE, Cass AEG, Gilardi G, 2002, High throughput assay for cytochrome P450BM3 for screening libraries of substrates and combinatorial mutants, BIOSENSORS & BIOELECTRONICS, Vol: 17, Pages: 119-131, ISSN: 0956-5663

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• Citations: 62

Zhang XE, Zhou YH, Zhang ZP, Xu HF, Shao WH, Cass AEGet al., 2002, Engineering <i>E-coli</i> alkaline phosphatase yields changes of catalytic activity, thermal stability and phosphate inhibition, BIOCATALYSIS AND BIOTRANSFORMATION, Vol: 20, Pages: 381-389, ISSN: 1024-2422

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• Citations: 6

Cass AEG, 2002, Biosensors, Encyclopedia of Molecular Medicine, New York, Publisher: Wiley, Pages: 369-371, ISBN: 9780471374947

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Hudson J, Cass AEG, Prabhananda BS, 2002, Synergy in K<SUP>+</SUP>/H<SUP>+</SUP> exchange across phospholipid vesicle membranes with combinations of valinomycin and chlorophenols, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 4, Pages: 2702-2707, ISSN: 1463-9076

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• Citations: 3

Topoglidis E, Cass AEG, O'Regan B, Durrant JRet al., 2001, Immobilisation and bioelectrochemistry of proteins on nanoporous TiO₂ and ZnO films, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, Vol: 517, Pages: 20-27, ISSN: 0022-0728

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• Citations: 268

Topoglidis E, Campbell CJ, Cass AEG, Durrant JRet al., 2001, Factors that affect protein adsorption on nanostructured titania films. A novel spectroelectrochemical application to sensing, LANGMUIR, Vol: 17, Pages: 7899-7906, ISSN: 0743-7463

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• Citations: 172

Zhou YF, Zhang XE, Liu H, Zhang ZP, Zhang CG, Cass AEGet al., 2001, Construction of a fusion enzyme system by gene splicing as a new molecular recognition element for a sequence biosensor, BIOCONJUGATE CHEMISTRY, Vol: 12, Pages: 924-931, ISSN: 1043-1802

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• Citations: 17

Cooper J, Disley D, Cass T, 2001, Microsystems special - Lab-on-a-chip and microarrays, CHEMISTRY & INDUSTRY, Pages: 653-655, ISSN: 0009-3068

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• Citations: 7

Halliwell CM, Morgan G, Ou CP, Cass AEGet al., 2001, Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules, ANALYTICAL BIOCHEMISTRY, Vol: 295, Pages: 257-261, ISSN: 0003-2697

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• Citations: 59

Zhou YF, Zhang XE, Liu H, Zhang CG, Cass AEet al., 2001, [Cloning and expression of Aspergillus niger glucose oxidase gene in methylotrophic yeast]., Sheng Wu Gong Cheng Xue Bao, Vol: 17, Pages: 400-405, ISSN: 1000-3061

The DNA fragment encoding A. niger glucose oxidase was amplified by PCR using A. niger genomic DNA as template, and was cloned into vector of pPIC9 for expression in Pichia pastoris. When transformed into methylotrophic yeast Pichia pastoris GS115, The constructed plasmid pPICGOD1 directed the synthesis and secretion of functionally active GOD. After induction in MM medium for 4 days, the GOD activity in the medium reached 30-40 u/mL. SDS-PAGE revealed that recombinant yeast GOD was expressed up to 60%-70% of the total soluble protein, and the secreted GOD could be purified to electrophoretic homogeneity with one purification step using Q Sepharose Fast Flow ion exchange chromatography. The recombinant yeast GOD had very high catalytic activity, showed about 1.6-fold increase of specific activity over the commercial A. niger GOD. Kinetic analysis clearly demonstrated that recombinant yeast GOD showed similar substrate affinity for glucose to A. niger GOD, but the turnover number of the GOD from yeast was determined to be much higher than that of A. niger GOD. In addition, the linear range of glucose electrode made with recombinant yeast GOD was efficiently widened due to the high catalytic activity of yeast GOD.

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Halliwell CM, Cass AEG, 2001, A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces, ANALYTICAL CHEMISTRY, Vol: 73, Pages: 2476-2483, ISSN: 0003-2700

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• Citations: 165

Zhang JK, Cass AEG, 2001, A study of his-tagged alkaline phosphatase immobilization on a nanoporous nickel-titanium dioxide film, ANALYTICAL BIOCHEMISTRY, Vol: 292, Pages: 307-310, ISSN: 0003-2697

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• Citations: 21

Cooper JM, Cass AEG, Yang H, 2001, Microfabricated biochip platforms for cell analyses, Biochip technology, Editors: Kricka, Cheng, Publisher: Harwood Academic, Pages: 309-324, ISBN: 9789057026133

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Zhou YF, Zhang XE, Liu H, Zhang CG, Cass AEet al., 2001, Cloning and expression of aspergillus niger glucose oxidase gene in methylotrophic yeast, Chinese Journal of Biotechnology (Chinese Edition), Vol: 17, Pages: 405-408, ISSN: 1000-3061

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Topoglidis E, Campbell CJ, Cass AEG, Durrant JRet al., 2001, Biosensors based on protein adsorption on nanoporous TiO2 films, Chemical and Biological sensors and analytical methods II, Editors: Butler, Vanysek, Yamazoe, Publisher: International Society of Electrochemistry, Pages: 196-202, ISBN: 9781566773515

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Shao WH, Zhang XE, Liu H, Zhang ZPet al., 2000, Anchor-chain molecular system for orientation control in enzyme immobilization, BIOCONJUGATE CHEMISTRY, Vol: 11, Pages: 822-826, ISSN: 1043-1802

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• Citations: 30

Cass T, 2000, Prism, SCIENCE, Vol: 289, Pages: 1158-1158, ISSN: 0036-8075

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• Citations: 4

Zhang JK, Cass AEG, 2000, Electrochemical analysis of immobilised chemical and genetic biotinylated alkaline phosphatase, ANALYTICA CHIMICA ACTA, Vol: 408, Pages: 241-247, ISSN: 0003-2670

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• Citations: 24

Glidle A, Hadyoon CS, Cass AEG, Cooper JMet al., 2000, Modifications of electrodeposited polymers by ion chelation to produce templates for biomolecule immobilisation, ELECTROCHIMICA ACTA, Vol: 45, Pages: 3823-3831, ISSN: 0013-4686

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• Citations: 12

Cass AEG, Valat C, Steel A, 2000, Immobilized Nucleic Acid Hybridization Reagent and Meth, WO042222A2

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