Imperial College London
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Professor Tony Cass

Faculty of Natural Sciences, Department of Chemistry

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Le:2017:10.1021/acs.analchem.7b01149,
author = {Le, T and Chang, P and Benton, DJ and McCauley, JW and Iqbal, M and Cass, AEG},
doi = {10.1021/acs.analchem.7b01149},
journal = {Analytical Chemistry},
pages = {6781--6786},
title = {Dual recognition element lateral flow assay (DRELFA) towards multiplex strain-specific influenza virus detection},
url = {http://dx.doi.org/10.1021/acs.analchem.7b01149},
volume = {89},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Different influenza virus strains have caused a number of recent outbreaks killing scores of people and causing significant losses in animal farming. Simple, rapid, sensitive, and specific detection of particular strains, such as a pandemic strain versus a previous seasonal influenza, plays a crucial role in the monitoring, controlling, and management of outbreaks. In this paper we describe a dual recognition element lateral flow assay (DRELFA) which pairs a nucleic acid aptamer with an antibody for use as a point-of-care platform which can detect particular strains of interest. The combination is used to overcome the individual limitations of antibodies’ cross-reactivity and aptamers’ slow binding kinetics. In the detection of influenza viruses, we show that DRELFA can discriminate a particular virus strain against others of the same subtype or common respiratory diseases while still exhibiting fast binding kinetic of the antibody-based lateral flow assay (LFA). The improvement in specificity that DRELFA exhibits is an advantage over the currently available antibody-based LFA systems for influenza viruses, which offer discrimination between influenza virus types and subtypes. Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is very effective in localizing the analyte to the test line (consistently over 90%) and this is crucial for the sensitivity of the device. In addition, color intensities of the test lines showed a good correlation between the DRELFA and the qRT-PCR over a 50-fold concentration range. Finally, lateral flow strips with a streptavidin capture test line and an anti-antibody control line are universally applicable to specific detection of a wide range of different analytes.
AU  - Le,T
AU  - Chang,P
AU  - Benton,DJ
AU  - McCauley,JW
AU  - Iqbal,M
AU  - Cass,AEG
DO  - 10.1021/acs.analchem.7b01149
EP  - 6786
PY  - 2017///
SN  - 1520-6882
SP  - 6781
TI  - Dual recognition element lateral flow assay (DRELFA) towards multiplex strain-specific influenza virus detection
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.7b01149
UR  - http://hdl.handle.net/10044/1/48848
VL  - 89
ER  -
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