Imperial College London
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Professor Tony Cass

Faculty of Natural Sciences, Department of Chemistry

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rawson:2017:10.1016/j.elecom.2017.07.011,
author = {Rawson, TM and Sharma, S and Georgiou, P and Holmes, A and Cass, A and O'Hare, D},
doi = {10.1016/j.elecom.2017.07.011},
journal = {Electrochemistry Communications},
pages = {1--5},
title = {Towards a minimally invasive device for beta-lactam monitoring in humans},
url = {http://dx.doi.org/10.1016/j.elecom.2017.07.011},
volume = {82},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (± 1SD) = 0.0044 ± 0.0013 M and mean slope function of log(concentration plots) 29 ± 1.80 mV/decade (r2 = 0.933). Response was reproducible after 28 days storage at 4 °C. In artificial ISF, the sensors response was Km (± 1SD) = 0.0077 ± 0.0187 M and a slope function of 34 ± 1.85 mv/decade (r2 = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor.
AU  - Rawson,TM
AU  - Sharma,S
AU  - Georgiou,P
AU  - Holmes,A
AU  - Cass,A
AU  - O'Hare,D
DO  - 10.1016/j.elecom.2017.07.011
EP  - 5
PY  - 2017///
SN  - 1388-2481
SP  - 1
TI  - Towards a minimally invasive device for beta-lactam monitoring in humans
T2  - Electrochemistry Communications
UR  - http://dx.doi.org/10.1016/j.elecom.2017.07.011
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000408359600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/52776
VL  - 82
ER  -
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