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@article{S:2018:10.3389/fmolb.2018.00074,
author = {S, Sgro G and Costa, TRD},
doi = {10.3389/fmolb.2018.00074},
journal = {Frontiers in Molecular Biosciences},
title = {Cryo-EM grid preparation of membrane protein samples for single particle analysis},
url = {http://dx.doi.org/10.3389/fmolb.2018.00074},
volume = {5},
year = {2018}
}

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TY  - JOUR
AB  - Recent advances in cryo-electron microscopy (cryo-EM) have made it possible to solve structures of biological macromolecules at near atomic resolution. Development of more stable microscopes, improved direct electron detectors and faster software for image processing has enabled structural solution of not only large macromolecular (megadalton range) complexes but also small (~60 kDa) proteins. As a result of the widespread use of the technique, we have also witnessed new developments of techniques for cryo-EM grid preparation of membrane protein samples. This includes new types of solubilization strategies that better stabilize these protein complexes and the development of new grid supports with proven efficacy in reducing the motion of the molecules during electron beam exposure. Here, we discuss the practicalities and recent challenges of membrane protein sample preparation and vitrification, as well as grid support and foil treatment in the context of the structure determination of protein complexes by single particle cryo-EM.
AU  - S,Sgro G
AU  - Costa,TRD
DO  - 10.3389/fmolb.2018.00074
PY  - 2018///
SN  - 2296-889X
TI  - Cryo-EM grid preparation of membrane protein samples for single particle analysis
T2  - Frontiers in Molecular Biosciences
UR  - http://dx.doi.org/10.3389/fmolb.2018.00074
UR  - http://hdl.handle.net/10044/1/67467
VL  - 5
ER  -

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