Imperial College London
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Dr Tiago Costa

Faculty of Natural Sciences, Department of Life Sciences

Senior Lecturer in Bacterial Pathogenesis
 
 
 
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Contact

 

+44 (0)20 7594 3696t.costa Website

 
 
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Location

 

5.02Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zheng:2021:10.1073/pnas.2022826118,
author = {Zheng, W and Peña, A and Ilangovan, A and Clark, JN-B and Frankel, G and Egelman, EH and Costa, TRD},
doi = {10.1073/pnas.2022826118},
journal = {Proceedings of the National Academy of Sciences of USA},
title = {Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament},
url = {http://dx.doi.org/10.1073/pnas.2022826118},
volume = {118},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC) utilize a macromolecular type III secretion system (T3SS) to inject effector proteins into eukaryotic cells. This apparatus spans the inner and outer bacterial membranes and includes a helical needle protruding into the extracellular space. Thus far observed only in EPEC and EHEC and not found in other pathogenic Gram-negative bacteria that have a T3SS is an additional helical filament made by the EspA protein that forms a long extension to the needle, mediating both attachment to eukaryotic cells and transport of effector proteins through the intestinal mucus layer. Here, we present the structure of the EspA filament from EPEC at 3.4 Å resolution. The structure reveals that the EspA filament is a right-handed 1-start helical assembly with a conserved lumen architecture with respect to the needle to ensure the seamless transport of unfolded cargos en route to the target cell. This functional conservation is despite the fact that there is little apparent overall conservation at the level of sequence or structure with the needle. We also unveil the molecular details of the immunodominant EspA epitope that can now be exploited for the rational design of epitope display systems.
AU  - Zheng,W
AU  - Peña,A
AU  - Ilangovan,A
AU  - Clark,JN-B
AU  - Frankel,G
AU  - Egelman,EH
AU  - Costa,TRD
DO  - 10.1073/pnas.2022826118
PY  - 2021///
SN  - 0027-8424
TI  - Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament
T2  - Proceedings of the National Academy of Sciences of USA
UR  - http://dx.doi.org/10.1073/pnas.2022826118
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33397726
UR  - https://www.pnas.org/content/118/2/e2022826118
UR  - http://hdl.handle.net/10044/1/103331
VL  - 118
ER  -
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