Publications

BibTex format

@article{Amin:2021:10.1038/s41467-021-27178-8,
author = {Amin, H and Ilangovan, A and Costa, TRD},
doi = {10.1038/s41467-021-27178-8},
journal = {Nat Commun},
title = {Architecture of the outer-membrane core complex from a conjugative type IV secretion system.},
url = {http://dx.doi.org/10.1038/s41467-021-27178-8},
volume = {12},
year = {2021}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Conjugation is one of the most important processes that bacteria utilize to spread antibiotic resistance genes among bacterial populations. Interbacterial DNA transfer requires a large double membrane-spanning nanomachine called the type 4 secretion system (T4SS) made up of the inner-membrane complex (IMC), the outer-membrane core complex (OMCC) and the conjugative pilus. The iconic F plasmid-encoded T4SS has been central in understanding conjugation for several decades, however atomic details of its structure are not known. Here, we report the structure of a complete conjugative OMCC encoded by the pED208 plasmid from E. coli, solved by cryo-electron microscopy at 3.3 Å resolution. This 2.1 MDa complex has a unique arrangement with two radial concentric rings, each having a different symmetry eventually contributing to remarkable differences in protein stoichiometry and flexibility in comparison to other OMCCs. Our structure suggests that F-OMCC is a highly dynamic complex, with implications for pilus extension and retraction during conjugation.
AU  - Amin,H
AU  - Ilangovan,A
AU  - Costa,TRD
DO  - 10.1038/s41467-021-27178-8
PY  - 2021///
TI  - Architecture of the outer-membrane core complex from a conjugative type IV secretion system.
T2  - Nat Commun
UR  - http://dx.doi.org/10.1038/s41467-021-27178-8
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34824240
UR  - http://hdl.handle.net/10044/1/92863
VL  - 12
ER  -

Download

Dr Tiago Costa

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)