Imperial College London
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Dr Tiago Costa

Faculty of Natural Sciences, Department of Life Sciences

Senior Lecturer in Bacterial Pathogenesis
 
 
 
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Contact

 

+44 (0)20 7594 3696t.costa Website

 
 
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Location

 

5.02Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Francis:2016:10.1007/978-1-4939-6649-3_2,
author = {Francis, MS and Amer, AAA and Milton, DL and Costa, TRD},
doi = {10.1007/978-1-4939-6649-3_2},
journal = {Methods Mol Biol},
pages = {11--31},
title = {Site-Directed Mutagenesis and Its Application in Studying the Interactions of T3S Components.},
url = {http://dx.doi.org/10.1007/978-1-4939-6649-3_2},
volume = {1531},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Type III secretion systems are a prolific virulence determinant among Gram-negative bacteria. They are used to paralyze the host cell, which enables bacterial pathogens to establish often fatal infections-unless an effective therapeutic intervention is available. However, as a result of a catastrophic rise in infectious bacteria resistant to conventional antibiotics, these bacteria are again a leading cause of worldwide mortality. Hence, this report describes a pDM4-based site-directed mutagenesis strategy that is assisting in our foremost objective to better understand the fundamental workings of the T3SS, using Yersinia as a model pathogenic bacterium. Examples are given that clearly document how pDM4-mediated site-directed mutagenesis has been used to establish clean point mutations and in-frame deletion mutations that have been instrumental in identifying and understanding the molecular interactions between components of the Yersinia type III secretion system.
AU  - Francis,MS
AU  - Amer,AAA
AU  - Milton,DL
AU  - Costa,TRD
DO  - 10.1007/978-1-4939-6649-3_2
EP  - 31
PY  - 2016///
SP  - 11
TI  - Site-Directed Mutagenesis and Its Application in Studying the Interactions of T3S Components.
T2  - Methods Mol Biol
UR  - http://dx.doi.org/10.1007/978-1-4939-6649-3_2
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27837478
VL  - 1531
ER  -
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