Imperial College London

DrTimothyEbbels

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Reader in Computational Bioinformatics
 
 
 
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Contact

 

+44 (0)20 7594 3160t.ebbels Website

 
 
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Location

 

131Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kaluarachchi:2018:10.1007/s11306-018-1332-1,
author = {Kaluarachchi, M and Boulangé, C and Karaman, I and Lindon, JC and Ebbels, T and Elliott, P and Tracy, R and Olson, NC},
doi = {10.1007/s11306-018-1332-1},
journal = {Metabolomics},
title = {A comparison of human serum and plasma metabolites using untargeted 1H NMR spectroscopy and UPLC-MS},
url = {http://dx.doi.org/10.1007/s11306-018-1332-1},
volume = {14},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Introduction:Differences in the metabolite profiles between serum and plasma are incompletely understood.Objectives:To evaluate metabolic profile differences between serum and plasma and among plasma sample subtypes.Methods:We analyzed serum, platelet rich plasma (PRP), platelet poor plasma (PPP), and platelet free plasma (PFP), collected from 8 non-fasting apparently healthy women, using untargeted standard 1D and CPMG 1H NMR and reverse phase and hydrophilic (HILIC) UPLC-MS. Differences between metabolic profiles were evaluated using validated principal component and orthogonal partial least squares discriminant analysis.ResultsExplorative analysis showed the main source of variation among samples was due to inter-individual differences with no grouping by sample type. After correcting for inter-individual differences, lipoproteins, lipids in VLDL/LDL, lactate, glutamine, and glucose were found to discriminate serum from plasma in NMR analyses. In UPLC-MS analyses, lysophosphatidylethanolamine (lysoPE)(18:0) and lysophosphatidic acid(20:0) were higher in serum, and phosphatidylcholines (PC)(16:1/18:2, 20:3/18:0, O-20:0/22:4), lysoPC(16:0), PE(O-18:2/20:4), sphingomyelin(18:0/22:0), and linoleic acid were lower. In plasma subtype analyses, isoleucine, leucine, valine, phenylalanine, glutamate, and pyruvate were higher among PRP samples compared with PPP and PFP by NMR while lipids in VLDL/LDL, citrate, and glutamine were lower. By UPLC-MS, PE(18:0/18:2) and PC(P-16:0/20:4) were higher in PRP compared with PFP samples.Conclusions:Correction for inter-individual variation was required to detect metabolite differences between serum and plasma. Our results suggest the potential importance of inter-individual effects and sample type on the results from serum and plasma metabolic phenotyping studies.
AU - Kaluarachchi,M
AU - Boulangé,C
AU - Karaman,I
AU - Lindon,JC
AU - Ebbels,T
AU - Elliott,P
AU - Tracy,R
AU - Olson,NC
DO - 10.1007/s11306-018-1332-1
PY - 2018///
SN - 1573-3882
TI - A comparison of human serum and plasma metabolites using untargeted 1H NMR spectroscopy and UPLC-MS
T2 - Metabolomics
UR - http://dx.doi.org/10.1007/s11306-018-1332-1
UR - http://hdl.handle.net/10044/1/56861
VL - 14
ER -