Imperial College London
Portrait Picture

Tom Ellis

Faculty of Engineering, Department of Bioengineering

Professor of Synthetic Genome Engineering
 
 
 
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Contact

 

+44 (0)20 7594 7615t.ellis Website CV

 
 
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Location

 

704Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pothoulakis:2018:10.1371/journal.pone.0194588,
author = {Pothoulakis, G and Ellis, T},
doi = {10.1371/journal.pone.0194588},
journal = {PLoS ONE},
title = {Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression},
url = {http://dx.doi.org/10.1371/journal.pone.0194588},
volume = {13},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Engineered promoters with predefined regulation are a key tool for synthetic biology that enable expression on demand and provide the logic for genetic circuits. To expand the availability of synthetic biology tools for S. cerevisiae yeast, we here used hybrid promoter engineering to construct tightly-controlled, externally-inducible promoters that only express in haploid mother cells that have contributed a daughter cell to the population. This is achieved by combining elements from the native HO promoter and from a TetR-repressible synthetic promoter, with the performance of these promoters characterized by both flow cytometry and microfluidics-based fluorescence microscopy. These new engineered promoters are provided as an enabling tool for future synthetic biology applications that seek to exploit differentiation within a yeast population.
AU  - Pothoulakis,G
AU  - Ellis,T
DO  - 10.1371/journal.pone.0194588
PY  - 2018///
SN  - 1932-6203
TI  - Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0194588
UR  - http://hdl.handle.net/10044/1/58046
VL  - 13
ER  -
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