Publications
413 results found
Kanagawa M, Liu Y, Hanashima S, et al., 2014, Structural Basis for Multiple Sugar Recognition of Jacalin-related Human ZG16p Lectin, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 289, Pages: 16954-16965
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- Citations: 28
Gao C, Liu Y, Zhang H, et al., 2014, Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin <i>O</i>-Glycome Designer Array, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 289, Pages: 16462-16477, ISSN: 0021-9258
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- Citations: 37
Nonaka M, Fukuda MN, Gao C, et al., 2014, Determination of Carbohydrate Structure Recognized by Prostate-specific F77 Monoclonal Antibody through Expression Analysis of Glycosyltransferase Genes, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 289, Pages: 16478-16486
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- Citations: 26
Khan ZM, Liu Y, Neu U, et al., 2014, Crystallographic and Glycan Microarray Analysis of Human Polyomavirus 9 VP1 Identifies <i>N</i>-Glycolyl Neuraminic Acid as a Receptor Candidate, JOURNAL OF VIROLOGY, Vol: 88, Pages: 6100-6111, ISSN: 0022-538X
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- Citations: 30
Falkowska E, Le KM, Ramos A, et al., 2014, Broadly Neutralizing HIV Antibodies Define a Glycan-Dependent Epitope on the Prefusion Conformation of gp41 on Cleaved Envelope Trimers, IMMUNITY, Vol: 40, Pages: 657-668, ISSN: 1074-7613
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- Citations: 280
York WS, Agravat S, Aoki-Kinoshita KF, et al., 2014, MIRAGE: The minimum information required for a glycomics experiment, GLYCOBIOLOGY, Vol: 24, Pages: 402-406, ISSN: 0959-6658
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- Citations: 84
Palma AS, Feizi T, Childs RA, et al., 2014, The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the <i>meta</i>-glycome, CURRENT OPINION IN CHEMICAL BIOLOGY, Vol: 18, Pages: 87-94, ISSN: 1367-5931
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- Citations: 69
Mulloy B, Feizi T, 2014, Tricks of the trade in glycoscience: the preparation and analysis of a blood group A-active mucin glycoprotein., Biochem J, Vol: 2014, Pages: c1-c3
Many aspects of glycosylation are conserved among animals, and it can be advantageous and sometimes critical to identify a readily available and abundant source of carbohydrate material that harbours a hard-to-characterize antigen or ligand of interest. The Biochemical Journal Classic paper by Morgan and King is a well-written account of serviceable methods for the extraction and quantification of a carbohydrate antigen. These methods were highly influential in subsequent studies of the blood group antigens. Some of these tricks of the trade still have a place in modern glycobiology.
Crusat M, Liu J, Palma AS, et al., 2013, Changes in the hemagglutinin of H5N1 viruses during human infection - Influence on receptor binding, VIROLOGY, Vol: 447, Pages: 326-337, ISSN: 0042-6822
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- Citations: 29
Cerqueira C, Liu Y, Kuehling L, et al., 2013, Heparin increases the infectivity of Human Papillomavirus Type 16 independent of cell surface proteoglycans and induces L1 epitope exposure, CELLULAR MICROBIOLOGY, Vol: 15, Pages: 1818-1836, ISSN: 1462-5814
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- Citations: 49
Palma AS, Pinheiro B, Liu Y, et al., 2013, The Structural Basis of the Recognition of Di-glucosylated N-glycans by the ER Lectin Malectin, Annual Conference of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1368-1369, ISSN: 0959-6658
Neu U, Khan ZM, Schuch B, et al., 2013, Structures of B-Lymphotropic Polyomavirus VP1 in Complex with Oligosaccharide Ligands, PLOS PATHOGENS, Vol: 9, ISSN: 1553-7366
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- Citations: 22
Neu U, Allen S-AA, Blaum BS, et al., 2013, A Structure-Guided Mutation in the Major Capsid Protein Retargets BK Polyomavirus, PLOS PATHOGENS, Vol: 9, ISSN: 1553-7366
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- Citations: 64
, 2013, Retraction: Oligosaccharide ligands for NKR-P1 protein activate NK cells and cytotoxicity., Nature, Vol: 500
Kong L, Lee JH, Doores KJ, et al., 2013, Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120, NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 20, Pages: 796-+, ISSN: 1545-9993
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- Citations: 269
Khan ZM, Neu U, Schuch B, et al., 2013, Crystal structures of the major capsid protein VP1 from human polyomavirus 9 and African green monkey polyomavirus provide a foundation for understanding the serological cross-reactivity between these two closely related polyomaviruses, 5th International Conference on Polyomaviruses and Human Diseases - Basic and Clinical Perspectives, Publisher: SPRINGER, Pages: 284-284, ISSN: 1355-0284
Feizi T, 2013, Carbohydrate recognition in the immune system: contributions of neoglycolipid-based microarrays to carbohydrate ligand discovery, ANNALS REPORTS, Vol: 1292, Pages: 33-44, ISSN: 0077-8923
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- Citations: 14
Reiss K, Stencel JE, Liu Y, et al., 2012, The GM2 Glycan Serves as a Functional Coreceptor for Serotype 1 Reovirus, PLOS PATHOGENS, Vol: 8, ISSN: 1553-7366
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- Citations: 72
Mouquet H, Scharf L, Euler Z, et al., 2012, Complex-type <i>N</i>-glycan recognition by potent broadly neutralizing HIV antibodies, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 109, Pages: E3268-E3277, ISSN: 0027-8424
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- Citations: 407
Marchant J, Cowper B, Liu Y, et al., 2012, Galactose recognition by the apicomplexan parasite Toxoplasma gondii. (vol 287, pg 16720, 2012), JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, Pages: 31455-31455, ISSN: 0021-9258
Orlowski A, Kaiser H-J, Rog T, et al., 2012, Role of membrane cholesterol in hydrophobic matching and the resulting redistribution of proteins and lipids, 22nd IUBMB Congress/37th FEBS Congress, Publisher: WILEY-BLACKWELL, Pages: 123-123, ISSN: 1742-464X
Khan Z, Neu U, Feizi T, et al., 2012, Structures of the major capsid protein (VP1) from closely related Human Polyomavirus-9 (HPyV-9) and African Green Monkey Polyomavirus (AGMPyV) provide insights into host range and tropism, 26th Annual Symposium of the Protein-Society, Publisher: WILEY-BLACKWELL, Pages: 117-118, ISSN: 0961-8368
Klein F, Gaebler C, Mouquet H, et al., 2012, Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein, JOURNAL OF EXPERIMENTAL MEDICINE, Vol: 209, Pages: 1469-1479, ISSN: 0022-1007
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- Citations: 132
Graham LM, Gupta V, Schafer G, et al., 2012, The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, Pages: 25964-25974
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- Citations: 94
Marchant J, Cowper B, Liu Y, et al., 2012, Galactose Recognition by the Apicomplexan Parasite <i>Toxoplasma gondii</i>, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, Pages: 16720-16733
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- Citations: 41
Palma AS, Zhang Y, Childs RA, et al., 2012, Neoglycolipid-Based "Designer" Oligosaccharide Microarrays to Define beta-Glucan Ligands for Dectin-1, Methods Mol.Biol., Vol: 808, Pages: 337-359
In this chapter, we describe the key steps of the "designer" oligosaccharide microarray approach we followed to prove the carbohydrate binding activity and define the oligosaccharide ligands for Dectin-1, an atypical C-type lectin-like signaling receptor of the mammalian innate immune system with a key role in anti-fungal immunity. The term "designer" microarray, which we introduced in the course of the Dectin-1 study refers to a microarray of oligosaccharide probes generated from ligand-bearing glycoconjugates to reveal the oligosaccharide ligands they harbor, so that these can be isolated and characterized. Oligosaccharide probes were generated from two polysaccharides, one that was bound by Dectin-1 and known to be rich in beta1,3-glucose sequence and another that was not bound and was rich in beta1,6-glucose sequence and served as a negative control. The approach involved: classic ELISA-type binding assays to select the polysaccharides; partial depolymerization of the polysaccharides by chemical hydrolysis; fractionation by size of the glucan oligosaccharides obtained and determination of their chain lengths by mass spectrometry; detection of Dectin-1 ligand-positive and ligand-negative oligosaccharides using the neoglycolipid (NGL) technology; methylation analysis of oligosaccharides to derive glucose linkage information, and incorporation of the newly generated glucan oligosaccharide probes into microarrays encompassing diverse mammalian-type and exogenous sequences for microarray analysis of Dectin-1
Liu Y, Childs RA, Palma AS, et al., 2012, Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses, Methods Mol.Biol., Vol: 808, Pages: 117-136
Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein-carbohydrate interactions both in endogenous recognition systems and pathogen-host interactions. We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features of the NGL-based oligosaccharide microarrays, and describes in detail the basic techniques - from the preparation of NGL probes to the generation of microarrays using robotic arraying hardware, as well as a general protocol for probing the microarrays with carbohydrate-binding proteins
Watson AA, Lebedev AA, Hall BA, et al., 2011, Structural flexibility and ligand-binding characteristics of the macrophage dengue virus receptor CLEC5A, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 101-101, ISSN: 0019-2805
Pejchal R, Doores KJ, Walker LM, et al., 2011, A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield, Science
The HIV envelope (Env) protein gp120 is protected from antibody recognition by a dense glycan shield. However, several of the recently identified PGT broadly neutralizing antibodies appear to interact directly with the HIV glycan coat. Crystal structures of Fabs PGT 127 and 128 with Man(9) at 1.65 and 1.29 A resolution, respectively, and glycan binding data delineate a specific high mannose binding site. Fab PGT 128 complexed with a fully glycosylated gp120 outer domain at 3.25 A reveals that the antibody penetrates the glycan shield and recognizes two conserved glycans as well as a short beta-strand segment of the gp120 V3 loop, accounting for its high binding affinity and broad specificify. Furthermore, our data suggest that the high neutralization potency of PGT 127 and 128 IgGs may be mediated by cross-linking Env trimers on the viral surface
Kaiser H-J, Orlowski A, Rog T, et al., 2011, Lateral sorting in model membranes by cholesterol-mediated hydrophobic matching, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 16628-16633, ISSN: 0027-8424
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- Citations: 115
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