Imperial College London
Alternate

ProfessorTenFeizi

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor and Director of the Glycosciences Laboratory
 
 
 
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Contact

 

+44 (0)20 7594 7207t.feizi

 
 
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Location

 

E518Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Silva:2021:10.1016/j.carbpol.2020.117350,
author = {Silva, LM and Correia, VG and Moreira, ASP and Domingues, MRM and Ferreira, RM and Figueiredo, C and Azevedo, NF and Marcos-Pinto, R and Carneiro, F and Magalhães, A and Reis, C and Feizi, T and Ferreira, JA and Coimbra, MA and Palma, AS},
doi = {10.1016/j.carbpol.2020.117350},
journal = {Carbohydrate Polymers},
title = {Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays},
url = {http://dx.doi.org/10.1016/j.carbpol.2020.117350},
volume = {253},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The structural diversity of the lipopolysaccharides (LPSs) from Helicobacter pylori poses a challenge to establish accurate and strain-specific structure-function relationships in interactions with the host. Here, LPS structural domains from five clinical isolates were obtained and compared with the reference strain 26695. This was achieved combining information from structural analysis (GC-MS and ESI-MSn) with binding data after interrogation of a LPS-derived carbohydrate microarray with sequence-specific proteins. All LPSs expressed Lewisx/y and N-acetyllactosamine determinants. Ribans were also detected in LPSs from all clinical isolates, allowing their distinction from the 26695 LPS. There was evidence for 1,3-d-galactans and blood group H-type 2 sequences in two of the clinical isolates, the latter not yet described for H. pylori LPS. Furthermore, carbohydrate microarray analyses showed a strain-associated LPS recognition by the immune lectins DC-SIGN and galectin-3 and revealed distinctive LPS binding patterns by IgG antibodies in the serum from H. pylori-infected patients.
AU  - Silva,LM
AU  - Correia,VG
AU  - Moreira,ASP
AU  - Domingues,MRM
AU  - Ferreira,RM
AU  - Figueiredo,C
AU  - Azevedo,NF
AU  - Marcos-Pinto,R
AU  - Carneiro,F
AU  - Magalhães,A
AU  - Reis,C
AU  - Feizi,T
AU  - Ferreira,JA
AU  - Coimbra,MA
AU  - Palma,AS
DO  - 10.1016/j.carbpol.2020.117350
PY  - 2021///
SN  - 0144-8617
TI  - Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays
T2  - Carbohydrate Polymers
UR  - http://dx.doi.org/10.1016/j.carbpol.2020.117350
UR  - http://hdl.handle.net/10044/1/85130
VL  - 253
ER  -
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