Imperial College London
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Dr Tiago C. Luis

Faculty of Medicine, Department of Immunology and Inflammation

Sir Henry Dale Fellow
 
 
 
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Contact

 

t.luis Website

 
 
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Location

 

9N4bCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Breitbach:2018:10.1016/j.stem.2018.01.008,
author = {Breitbach, M and Kimura, K and Luis, TC and Fuegemann, CJ and Woll, PS and Hesse, M and Facchini, R and Rieck, S and Jobin, K and Reinhardt, J and Ohneda, O and Wenzel, D and Geisen, C and Kurts, C and Kastenmueller, W and Hoelzel, M and Jacobsen, SEW and Fleischmann, BK},
doi = {10.1016/j.stem.2018.01.008},
journal = {Cell Stem Cell},
pages = {262--+},
title = {In vivo labeling by CD73 marks multipotent stromal cells and highlights endothelial heterogeneity in the bone marrow niche},
url = {http://dx.doi.org/10.1016/j.stem.2018.01.008},
volume = {22},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Despite much work studying ex vivo multipotent stromal cells (MSCs), the identity and characteristics of MSCs in vivo are not well defined. Here, we generated a CD73-EGFP reporter mouse to address these questions and found EGFP+ MSCs in various organs. In vivo, EGFP+ mesenchymal cells were observed in fetal and adult bones at proliferative ossification sites, while in solid organs EGFP+ cells exhibited a perivascular distribution pattern. EGFP+ cells from the bone compartment could be clonally expanded ex vivo from single cells and displayed trilineage differentiation potential. Moreover, in the central bone marrow CD73-EGFP+ specifically labeled sinusoidal endothelial cells, thought to be a critical component of the hematopoietic stem cell niche. Purification and molecular characterization of this CD73-EGFP+ population revealed an endothelial subtype that also displays a mesenchymal signature, highlighting endothelial cell heterogeneity in the marrow. Thus, the CD73-EGFP mouse is a powerful tool for studying MSCs and sinusoidal endothelium.
AU  - Breitbach,M
AU  - Kimura,K
AU  - Luis,TC
AU  - Fuegemann,CJ
AU  - Woll,PS
AU  - Hesse,M
AU  - Facchini,R
AU  - Rieck,S
AU  - Jobin,K
AU  - Reinhardt,J
AU  - Ohneda,O
AU  - Wenzel,D
AU  - Geisen,C
AU  - Kurts,C
AU  - Kastenmueller,W
AU  - Hoelzel,M
AU  - Jacobsen,SEW
AU  - Fleischmann,BK
DO  - 10.1016/j.stem.2018.01.008
EP  - 262
PY  - 2018///
SN  - 1934-5909
SP  - 262
TI  - In vivo labeling by CD73 marks multipotent stromal cells and highlights endothelial heterogeneity in the bone marrow niche
T2  - Cell Stem Cell
UR  - http://dx.doi.org/10.1016/j.stem.2018.01.008
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000423840400016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S1934590918300080?via%3Dihub
UR  - http://hdl.handle.net/10044/1/109145
VL  - 22
ER  -
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