Imperial College London
Alternate

Professor Toby Maher

Faculty of Medicine, National Heart & Lung Institute

Professor of Interstitial Lung Disease
 
 
 
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Contact

 

+44 (0)20 7594 2151t.maher

 
 
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Assistant

 

Ms Georgina Moss +44 (0)20 7594 2151

 
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Location

 

364Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Invernizzi:2021:10.1164/rccm.202002-0460OC,
author = {Invernizzi, R and Wu, BG and Barnett, J and Ghai, P and Kingston, S and Hewitt, RJ and Feary, J and Li, Y and Chua, F and Wu, Z and Wells, AU and George, PM and Renzoni, EA and Nicholson, AG and Rice, A and Devaraj, A and Segal, LN and Byrne, AJ and Maher, TM and Lloyd, CM and Molyneaux, PL},
doi = {10.1164/rccm.202002-0460OC},
journal = {Am J Respir Crit Care Med},
pages = {339--347},
title = {The Respiratory Microbiome in Chronic Hypersensitivity Pneumonitis Is Distinct from That of Idiopathic Pulmonary Fibrosis.},
url = {http://dx.doi.org/10.1164/rccm.202002-0460OC},
volume = {203},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rationale: Chronic hypersensitivity pneumonitis (CHP) is a condition that arises after repeated exposure and sensitization to inhaled antigens. The lung microbiome is increasingly implicated in respiratory disease, but, to date, no study has investigated the composition of microbial communities in the lower airways in CHP.Objectives: To characterize and compare the airway microbiome in subjects with CHP, subjects with idiopathic pulmonary fibrosis (IPF), and control subjects.Methods: We prospectively recruited individuals with a CHP diagnosis (n = 110), individuals with an IPF diagnosis (n = 45), and control subjects (n = 28). Subjects underwent BAL and bacterial DNA was isolated, quantified by quantitative PCR and the 16S ribosomal RNA gene was sequenced to characterize the bacterial communities in the lower airways.Measurements and Main Results: Distinct differences in the microbial profiles were evident in the lower airways of subjects with CHP and IPF. At the phylum level, the prevailing microbiota of both subjects with IPF and subjects with CHP included Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. However, in IPF, Firmicutes dominated, whereas the percentage of reads assigned to Proteobacteria in the same group was significantly lower than the percentage found in subjects with CHP. At the genus level, the Staphylococcus burden was increased in CHP, and Actinomyces and Veillonella burdens were increased in IPF. The lower airway bacterial burden in subjects with CHP was higher than that in control subjects but lower than that of those with IPF. In contrast to IPF, there was no association between bacterial burden and survival in CHP.Conclusions: The microbial profile of the lower airways in subjects with CHP is distinct from that of IPF, and, notably, the bacterial burden in individuals with CHP fails to predict survival.
AU  - Invernizzi,R
AU  - Wu,BG
AU  - Barnett,J
AU  - Ghai,P
AU  - Kingston,S
AU  - Hewitt,RJ
AU  - Feary,J
AU  - Li,Y
AU  - Chua,F
AU  - Wu,Z
AU  - Wells,AU
AU  - George,PM
AU  - Renzoni,EA
AU  - Nicholson,AG
AU  - Rice,A
AU  - Devaraj,A
AU  - Segal,LN
AU  - Byrne,AJ
AU  - Maher,TM
AU  - Lloyd,CM
AU  - Molyneaux,PL
DO  - 10.1164/rccm.202002-0460OC
EP  - 347
PY  - 2021///
SP  - 339
TI  - The Respiratory Microbiome in Chronic Hypersensitivity Pneumonitis Is Distinct from That of Idiopathic Pulmonary Fibrosis.
T2  - Am J Respir Crit Care Med
UR  - http://dx.doi.org/10.1164/rccm.202002-0460OC
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32692582
UR  - http://hdl.handle.net/10044/1/81112
VL  - 203
ER  -
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